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Validated All-in-One™ qPCR Primer for ADH1B(NM_000668.5) Search again
Product ID:
HQP002331
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ADH2, HEL-S-117
Gene Description:
alcohol dehydrogenase 1B (class I), beta polypeptide
Target Gene Accession:
NM_000668.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq].
Gene References into function
- Sructural aspects that influence dimer-tetramer formation
- There was a gene-environment interaction between the ADH2 polymorphism, alcohol consumption, and breast cancer risk. Breast cancer risk associated with alcohol consumption vary with the ADH2 polymorphism, probably due to differences in EtOH metabolism.
- The ADH2*2 allele protects against alcohol dependence severity in Jewish samples.
- Mutations that contribute to the risk of alcoholism affect European, Chinese, and Japanese populations differently.
- Polymorphisms of alcohol-metabolizing enzymes: analyses of mutations on the CYP2E1, ADH2, ADH3 and ALDH2 genes in a Mexican-American population living in the Los Angeles area.
- There is no significant interaction between alcohol consumption and ADH2 genotype.
- Polymorphism is not associated with laryngeal cancer risk in Caucasians.
- The alcohol-BP relationship was significantly stronger in men with ADH21/21 than in men with ADH21/22 or 22/22 in this Japanese rural population.
- The ADH1B gene may interact with the dopamine D2 receptor (DRD2) gene in the development of anxiety-depressive alcohol dependence in the Taiwan Han Chinese population.
- Risk of cerebral infarction is increased by the ADH2*1 allele.
- Effect modification suggested of association between alcohol consumption and breast cancer risk by ADH1B genotype
- Polymorphisms of the alcohol metabolism-related ADH2 gene are significantly different in Korean patients with alcoholism and Korean control subjects without alcoholism.
- polymorphisms in ADH1C and ADH1B may have roles in the risk of alcoholism
- Heterozygous alcohol dehydrogenase 2 genotype (ADH2*1/2*2) exhibited an association with the scant pancreatitis subgroup (P = 0.02).
- No effects of the ADH1B*47His alleles on in vivo ethanol metabolism were observed. This implies that there is a major determinant of variation for in vivo alcohol metabolism in the ADH region that is not accounted for by this polymorphism.
- interaction between ALDH2 and ADH2 polymorphisms may have a role in colorectal cancer in Japan
- ADH1B*2 allele has been associated with lowr rates of alcohol dependence.
- Data are consistent with the hypothesis that the maternal ADH1B*3 allele provides some protection to the fetus from prenatal alcohol exposure.
- findings suggest that the alcohol dehydrogenase IB and IC genes are regulated by epigenetic mechanisms in human hepatoma cells
- Results suggest that ADH1B polymorphisms play a pivotal role on esophageal cancer and that the effect of these polymorphisms was modified by the amount of alcohol consumed.
- Genetic polymorphisms of ADH1B is associated with oral and pharyngeal squamous cell carcinoma
- Results suggest, in this sample of Trinidadians, that the ADH1B(*)3 allele is associated with protection from the development of alcoholism and enhanced risk for elevated serum ALT levels in those individuals who do become alcohol dependent.
- Polymorphisms in ADH1B is associated with breast cancer
- The metabolism of all-trans- and 9-cis-retinol/ retinaldehyde has been investigated with focus on the activities of human, mouse and rat alcohol dehydrogenase 2 (ADH2), an intriguing enzyme with apparently different functions in human and rodents.
- the eNOS gene T-786C mutation and the fast form of ADH2 Arg47His polymorphism may have an additive interaction on the risk of premature coronary artery disease in Chinese population
- Low levels of alcohol are associated with a modest increase in breast cancer risk that is not altered by known functional allelic variants of the ADH1B gene.
- Higher levels of ethanol persisted in the blood for longer periods in ADH1B*1/*1 carriers (n = 25) than in ADH1B*2 allele carriers after adjustment for the amount and time of the preceding alcohol consumption and body weight
- 47Arg polymorphism in the ADH2 may act to suppress osteophyte formation unaffected by disc degeneration
- increased risk of fetal alcohol syndrome for the maternal and possibly fetal ADH1B/ADH1B genotype
- Alcohol dehydrogenase 2 genotype and allelic variants are not associated with the risk for essential tremor.
- The ADH1B*47His polymorphism reaches high frequencies only in western and eastern Asia
- This study suggests that the ALDH2*A allele status may correlate with variations in alcohol consumption patterns among Jews, independent of the effect of ADH1B genotype.
- men and women with ADH1B slow vs fast alcohol degradation drank more alcohol and had a higher risk of everyday drinking, heavy drinking, excessive drinking and of alcoholism.
- ADH2 and ALDH2 genotypes are associated with esophageal cancer risk. ADH2 1 allele and ALDH2 2 allele carriers have a much higher risk of developing esophageal cancer, especially among alcohol drinkers.
- the interplay of the ADH1B and ALDH2 genotypes, in conjunction with a behaved drinking habit and a practiced drinking pattern, along with continued tobacco consumption, plays an important pathogenic role in modulating esophageal SCC risk
- erythema following alcohol exposure is alcohol type specific, has a pharmacogenetic basis related to ADHIB haplotype
- In Japanese men with alcohol dehydrogenase 2*1/1 genotype there was a higher fasting plasma glucose level compared to those without the genotype.
- The low activity ALDH2*2 allele was significantly associated with increased risk for oesophageal cancer amongst the Black subjects
- describes demographic, alcohol use, country of origin, family history of alcohol dependence, and ALDH2 and ADH1b variables to elucidate response to alcohol amont Chinese and Korean-American college students
- Alcohol consumption and polymorphisms in the CYP2E1, ADH1B and ALDH2 genes are important risk factors for esophageal squamous cell carcinoma in Chinese males living in Gansu Province, China.
- Variants of this alcohol-metabolizing enzyme appeared in the relation to alcoholic chronic pancreatitis
- A high frequency of the ADH1B*47His allele is strongly correlated with certain eastern ethnic groups.
- Single Nucleotide Polymorphism in ADH1B gene is associated with upper aerodigestive cancer.
- Genetic polymorphism of the gene is substantially involving in individual variation of susceptibility to alcohol dependence.
- ALDH2 and ADH1B polymorphisms were associated with changes in blood catecholamine levels and cardiovascular measures after alcohol ingestion.
- Polymorphisms of the ADH2 and ALDH2 genes are significantly associated with colorectal cancer risk in Chinese males
- The fast metabolizing ADH1b 47his allele seemed to be associated with nondrinking in a Caucasian population
- ADH1B-Arg48His polymorphism affects both alcohol-related flushing in Europeans and alcohol intake.
- ADH1b47his allele frequency is different for European regions.
- Current data lend support to a role of polymorphisms ADH1B and ALDH2 combined with alcohol consumption in cancer