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Validated All-in-One™ qPCR Primer for PLIN2(NM_001122.3) Search again
Product ID:
HQP002234
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ADFP, ADRP
Gene Description:
perilipin 2
Target Gene Accession:
NM_001122.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation [provided by RefSeq].
Gene References into function
- induction by hypoxia
- targeting of the lipid droplet-binding adipocyte differentiation-related protein.
- Expression of adipophilin is enhanced during trophoblast differentiation and is up-regulated by ligand-activated PPARgamma/retinoid X receptor. May contribute to fatty acid uptake by placenta.
- Stimulation of adipophilin expression in macrophages by modified LDL promotes triglyceride & cholesterol storage and reduces cholesterol efflux. Adipophilin might contribute in vivo to lipid accumulation in the intima of the arterial wall.
- GDP-bound ARF1 induces dissociation of ADRP from the Lipid droplet surface;.
- TIP47 and adipophilin are components of many, but not all, the lipid droplets in THP-1 cells
- ADRP and TG regulate each other, and the ubiquitin-proteasome system is involved in degradation of ADRP during regression of lipid-storing cells.
- ADFP mRNA and protein are increased by long-chain polyunsaturated fatty acids in a human placental choriocarcinoma cell line (BeWo) and in primary human trophoblasts. The ADFP protein might facilitate transplacental transfer of maternal fatty acids
- Data demonstrate that adipose differentiation-related protein (ADRP) mRNA and protein are regulated by fatty acids in a human placental choriocarcinoma cell line (BeWo) and in primary human trophoblasts.
- We demonstrate that ADFP is transcriptionally regulated by peroxisome proliferator-activated receptor alpha (PPARalpha) in human hepatoma cells through a highly conserved direct repeat-1(DR-1) element.
- The results suggested that the putative hydrophobic cleft is critical for the unique characteristics of TIP47.
- Adipophilin expression in THP-1 macrophages altered the cellular content of different lipids and enhanced the size of lipid droplets
- adipose differentiation-related protein does not appear to have a role in lung surfactant production
- ADFP expression may have a role in clear cell renal carcinoma differentiation
- the metallothionein genes, adipophilin (ADFP), CD36, adipocyte fatty acid binding protein (FABP4), ATP binding cassette protein A1 (ABCA1), and liver X receptor (LXR[alpha]) all emerged as strongly positively correlated with PPAR[gamma] expression.
- Because adipophilin blocks cholesterol efflux from lipid-laden cells, they may die and develop a necrotic lipid core, thereby destabilizing the symptomatic carotid plaque.
- agLDL-LRP1 engagement induces adipocyte differentiation-related protein overexpression in both monocyte-derived macrophages and human vascular smooth muscle cells
- Perilipin content decreased and adipophilin increased with lipoprotein lipid loading regardless of intracellular neutral lipid composition
- Mutations in known genes account for 58% of autosomal dominant retinitis pigmentosa (adRP).
- Adipophilin and TIP47 are expressed in lipid droplets of vitamin A-storing hepatic stellate cells and additionally in lipid droplets of steatotic hepatocytes.
- Increased expression by OxLDL in PMA-differentiated THP-1 macrophages and in plaque vs. nonplaque lesion areas in human carotid endarterectomy specimens (it(correlated with CD36 expression).
- The present results suggest that lipid droplets (LD) and LD-associated proteins have protective effects against apoptosis induced by fatty acid-bound albumin by sequestering free fatty acids.
- Mycobacterium leprae regulates ADRP/perilipin expression to facilitate the accumulation of lipids within infected macrophages for intracellular survival.