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Validated All-in-One™ qPCR Primer for CCR4(NM_005508.4) Search again
Product ID:
HQP002209
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5
Gene Description:
C-C motif chemokine receptor 4
Target Gene Accession:
NM_005508.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq].
Gene References into function
- The human CCR4 gene is localized at Chr 3p24.
- CCR4 is expressed with high frequency in adult T-cell leukemia and human T-cell leukemia virus type 1-transformed T cells and in ATL skin lesions.
- CCR4 is mainly expressed by a high cytokine (interleukin-4/interleukin-2)-producing (CD4) subset of natural killer t-cells.
- Selective CCL5/RANTES-induced mast cell migration through interactions with chemokine receptors
- The skin-homing TH compartment is itself divided into distinct subpopulations, the smaller of which expresses both CCR4 and CCR10, and the larger of which expresses only CCR4.
- CCR4+ cells were present predominantly in the lesional skin of atopic dermatitis patients, but not in the non-lesional skin
- In the blood of cutaneous T cell lymphoma patients with peripheral blood involvement we found significantly increased percentages of T cells displaying the skin-homing phenotype (CLA+CCR4+) compared with healthy individuals.
- Increased expression of CCR4, which is proposed to guide CD25(+) Ts cells to DC, is an intrinsic feature of CD25(+) Ts cells.
- airway allergen-specific T(H)2 cells are CCR4(+), but in the atopic child CCR4 does not distinguish between recall antigen and allergen specificity
- although there is PI(3,4,5)P(3) accumulation downstream of CCR4, phosphoinositide 3-kinase activity is a dispensable signal for CCR4-stimulated chemotaxis of Th2 cells and the CEM T cell line.
- CXCR3 and CCR4 were heterogeneously expressed in peripheral T-cell lymphomas.
- CC chemokine receptor 4 has a role in adult T-Cell leukemia/lymphoma
- review of possible relationship of CCR4 role in T cell migration and skin infiltration in ATL, and of selective expression of CCR4 by Th2 and regulatory T cells and possible origin of ATL in Th2 or regulatory T cells.
- CCR4 and TARC/CCL17 play role in pathophysiology of cutaneous lupus erythematosus(CLE). Cytotoxic CD8+ T cells expressing CCR4 appear to be involved in scarring subtypes of CLE.
- CCR4 and CCR10 may play an important role in ATLL invasion into the skin
- Bexarotene reduces CCR4-positive lymphocytes.
- aberrantly expressed Fra-2 in association with JunD may play a major role in CCR4 expression and oncogenesis in adult T-cell leukemia.
- CCR4 and CCR10 are expressed on epidermal keratinocytes and that both are functional in terms of skin cytokine production and/or migration to their ligand CCL17 and CCL27, respectively.
- ratio of CCR4+/CXCR3+ cells was 4.45 in chemotherapy and 0.72 in immunotherapy
- Regulatory T cells recruited through CCL22/CCR4 are selectively activated in lymphoid infiltrates surrounding primary breast tumors and lead to an adverse clinical outcome.