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Validated All-in-One™ qPCR Primer for CKS1B(NM_001826.2) Search again
Product ID:
HQP001884
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CKS1, PNAS-16, PNAS-18, ckshs1
Gene Description:
CDC28 protein kinase regulatory subunit 1B
Target Gene Accession:
NM_001826.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
CKS1B protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
Gene References into function
- Weak cooperativity in the core causes a switch in folding mechanism between two proteins of the cks family.
- induction of Skp2 and Cks1 degradation in G1 represents a principal mechanism by which APC/C(Cdh1) prevents the unscheduled degradation of SCF(Skp2-Cks1) substrates and maintains the G1 state
- Cks1 overexpression may play an important role for oral squamous cell carcinoma development through Skp2-mediated p27 degradation
- analysis of the protein dynamics of Cks1
- ubiquitin ligase subunit Cks1 is involved in p27Kip1 down-regulation and may have an important role in the development of aggressive tumor behavior in breast cancer.
- over-expression of CKS1B, mainly due to gene amplification, imparts a poor prognosis in MM, possibly as a result of enhanced degradation of p27Kip1.
- Cks1 expression level regulates melanoma cell growth, most likely through effects on cell proliferation.
- Results show that complex formation between Cks1 and Skp2 causes conformational changes in both proteins in regions distant from the respective binding sites.
- CKS1B influences myeloma cell growth and survival through SKP2- and p27(Kip1)-dependent and -independent mechanisms
- Generally, the observation that the potential oncogene Cks1 is downregulated by the tumor suppressor p53 corresponds well with the idea that p53 employs multiple ways in order to halt the cell cycle.
- Myc targets CKS1 to provoke the suppression of KIP1.
- Data show that Cdc20 and Cks1 direct the spindle checkpoint-independent destruction of cyclin A2.
- Cks1 expression was only correlated with tumor size in renal cell carcinoma.
- These data suggest that Cks1 is an oncogene in the 1q21 amplicon and plays an important role for breast cancer development.