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Validated All-in-One™ qPCR Primer for CHRNA4(NM_000744.6) Search again
Product ID:
HQP001637
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BFNC, EBN, EBN1, NACHR, NACHRA4, NACRA4
Gene Description:
cholinergic receptor nicotinic alpha 4 subunit
Target Gene Accession:
NM_000744.6(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor.
Gene References into function
- Changes in nicotinic acetylcholine receptor subunits expression in brain of patients with Down syndrome and Alzheimer's disease.
- How mutations in the nAChRs can cause autosomal dominant nocturnal frontal lobe epilepsy
- Mutations of the gene encoding CHRNA4 may be linked to nocturnal frontal lobe epilepsy.
- A significant association was found for a 5' intron 2 single nucleotide polymorphism and severe inattention problems
- Correlative analysis between oxidative stress and deficit of nAChR alpha4 subunit indicates that increased lipid peroxidation correlates well with the decreased level of alpha4 subunit in Alzheimer's disease brains.
- CHRNA4 subunit is expressed in the soma of the majority of pyramidal cells, with the most alpha 4 immunoreactivity observed in CA2-4 and entorhinal cortex and relatively less in CA1 and subicular pyramidal cell soma.
- we examined the phenotypes in two families, from the same ethnic and geographic backgrounds, with ADNFLE as a result of mutations in these two different subunits of CHRN.
- significant decrease of the alpha4 and the alpha7 nicotinic acetylcholine receptor subunit in cortices of Parkinson patients which turns out to be similar to recent findings in Alzheimer patients.
- the alpha4 subunit of human alpha4beta2 nicotinic receptors is phosphorylated in situ by PKA and PKC.
- This study demonstrated an association between the CHRNA4 gene and FCs. Individuals with the T allele had a higher incidence of FCs. These data suggest that the CHRNA4 gene or a closely linked gene might be one of the susceptibility factors for FCs.
- Evidence for S284L mutation of the CHRNA4 in a white family with autosomal dominant nocturnal frontal lobe epilepsy.
- Reduced gene expression of the alpha4beta2 nicotinic receptor in the cerebral cortex is a major feature of the neurochemical pathology of autism, whilst post-transcriptional abnormalities of both this and the alpha7 subtype are apparent in the cerebellum
- A common haplotype of the nicotine acetylcholine receptor alpha 4 subunit gene is associated with vulnerability to nicotine addiction in men
- Authors' findings provide convincing evidence for the involvement of CHRNA4 in nicotine addiction.
- An association has been detected between the CfoI restriction fragment length polymorphism of the CHRNA4 receptor and alcoholism in Korean patients.
- nicotinic modulation of thalamo-cortical circuitry as a key component in the control of conscious awareness in dementia with Lewy bodies
- M1 domains on both alpha4 and beta2 play an important role for efficient expression of extracellular domain alpha4beta2 nAChRs that are high fidelity structural models of full-length alpha4beta2 nAChR
- nicotine-induced up-regulation of alpha4beta2 acetylcholine receptors resulted primarily from an increase in assembly from large pools of unassembled subunits, but up-regulation also resulted from a 5-fold increase in the lifetime in the surface membrane
- In Alzheimer's disease, CHRNA4 polymorphisms and the extent of dementia seem to affect the levels of DNA oxidative damage as well as to activate factors that participate in the DNA degradation and its repair.
- analysis of single channel conductance within the large cytoplasmic loop of 5-hydroxytryptamine type 3 and alpha4beta2 nicotinic acetylcholine receptors
- Our present study provided the first line of direct evidence suggesting that the CHRNA4 gene combined with CHRNB2 receptor gene may be linked to schizophrenia.
- These findings demonstrate that human nAChR beta2 or beta4 subunits can combine with alpha4 subunits to generate two forms of alpha4*-nAChR with distinctive physiological and pharmacological features.
- The interaction was stronger in the middle-aged than in the older participants. There was a trend for individuals with combined APOE-epsilon4/CHRNA4 TT genotypes to show both lower white matter volume and slower overall RT on the attention task.
- Analysis of six SNPs in the CHRNA4 gene provided modest support for an association with past 6 month use of alcohol in Caucasians (three SNPs with P < 0.08), but no evidence for an association with tobacco and CHRNA4 was detected.
- This study demonstrates that the CHRNA4 gene may be one of the susceptibility factors for idiopathic generalized epilepsy.
- We found no significant difference in RNFL thickness at the optic disc in the different genotype carriers of the APOE and CHRNA4 genes, and thereby no evidence for increased loss of ganglion cells in the retina as an effect of these genes
- Based on our results and others, CHRNA4 may be involved in ADHD; however, its role in ADHD symptomatology remains to be clarified.
- The auditory N1 component amplitude was higher for T allele homozygotes than for C allele carriers in CHRNA4. The visual P1 component revealed the same pattern of significant polymorphic modulation.
- first evidence that attentional network function may be modulated by genetic variations within CHRNA4 exon 5.
- Results of analyses ranging from basic biological approaches to clinical outcome data provide consistent evidence that 2 single-nucleotide polymorphisms in CHRNA4 are functional at a biological level and are associated with nicotine dependence phenotypes
- did not demonstrate a significant association between methylphenidate treatment response and the polymorphisms DRD4 VNTR, CHRNA4 (rs1044396 and rs6090384) and the long (L(A) and L(G)) and short (S) forms of the serotonin transporter promoter region
- Up-regulation of [(3)H]epibatidine binding in HEK293 cells stably expressing alpha4beta2-nAChR is significantly enhanced by co-application of tumor necrosis factor alpha.
- discussion of the role of variability in the genes that encode the two major brain-expressed alpha subunits, alpha4 and alpha7, in modulating behavior, physiology and disease risk in both humans and mice [review]
- subjects with the alpha4-Ser248Phe mutation mutation were shorter & had a greater body mass index than healthy volunteers & unaffected members of the pedigree; findings suggest a role of the nACh receptor in human growth regulation
- results do not demonstrate a significant genetic difference in 7 markers from the CHRNA4 and CHRNB2 genes between schizophrenia patients who smoke and those who do not
- CHRNA4 and CHRNB2 do not play a major role in Japanese schizophrenia.