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Validated All-in-One™ qPCR Primer for CHRM1(NM_000738.2) Search again
Product ID:
HQP001526
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
HM1, M1, M1R
Gene Description:
cholinergic receptor muscarinic 1
Target Gene Accession:
NM_000738.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation.
Gene References into function
- Fifteen single nucleotide polymorphisms (SNPs) of CHRM1 have been discovered, 9 of which are located in the coding region of the receptor; of these, 8 represent synonymous SNPs, indicating that CHRM1 is highly conserved in humans.
- Expression of this receptor is decreased in the dorsolateral prefrontal cortex of subjects with schizophrenia.
- 28% decrease in post-mortem levels of steady-state cortical muscarinic 1 receptor cDNA in schizophrenia patient samples relative to controls
- role of M1 and M2 muscarinic receptors expressed by human L cells in the control of GLP-1 secretion.
- In conclusion, autoantibodies to CHRM1 were detected in a large number of CFS patients and were related to CFS symptoms.
- M1- and M3- but not M2- or M4-AchR signals activate HIF-1 by both stabilization and synthesis of HIF-1alpha and by inducing the transcriptional activity of HIF-1alpha.
- All three of the Kir2 channels are similarly inhibited by m1 muscarinic receptor stimulation through calcium-dependent activation of the small GTPase Rho.
- Increased phosphorylation of M1 and M2 mAChRs underlies sequestration of these receptors after transient hypoxia. Distinct pathways involving CK1alpha and GRK2 mediated sequestration of M1 and M2 mAChRs after transient hypoxic-induced oxidative stress.
- Data demonstrate that M(1), M(2), and M(3) muscarinic acetylcholine receptors (mAChR)can form homo- and heterodimers in living cells, and suggest that heterodimerization plays a role in the mechanism of mAChR long term regulation.
- Data show that intracellular zinc uptake in SK-SH-SY5Y cells is controlled by M1-mAChR mediated signalling pathways and that zinc may act as a cofactor for transcriptional regulation of zinc finger genes such as PNUTS.
- MT7 toxin interacts with hM1 receptor at a specific allosteric site, which may partially overlap those identified previously for "classic" or "atypical" allosteric agents.
- Association of the nicotinic acetylcholine receptor beta1 subunit (CHRNB1) and M1 muscarinic acetylcholine receptor (CHRM1) with vulnerability for nicotine dependence.
- mAChR subtypes (m1 to m5) presents in human scleral fibroblasts at both mRNA and protein levels.
- This study provides the structural insight for improved understanding of M1 receptor and its binding sites.
- Gene Coding the human cholinergic receptor muscarinic-1 is an important susceptibility locus for asthma at chromosome 11q13.
- results demonstrate the ability of beta-arrestins to recruit diacylglycerol kinases to ligand-activated M1 muscarinic receptors
- M1r-IR in cholinergic and nitrergic neurons and nerve fibres indicate that M1rs have a role in both cholinergic and nitrergic transmission.
- The observations suggest that Cysteine pairs Cys259/Cys260 and Cys262/Cys263 in the 3rd intracellular loop play an important role in the agonist-induced internalization of muscarinic M1 receptors.
- M1 muscarinic and alpha7 nicotinic receptors have roles in inhibition of pemphigus acantholysis
- Certain Sjogren's syndrome individuals may have antibodies against M1R, M2R and M3R.
- The observed effect of tau protein on neurons (in neuroblastomas and primary cultures of hippocampal and cortical neurons) is through M1 and M3 muscarinic receptors.
- In progressive supranuclear palsy: there were no changes in M1 muscarinic receptor density or M1 coupling
- The M(1) receptor is the predominant mAChR subtype coupling to the Galpha(q/11) G protein in the cerebral cortex.
- Results demonstrate that the actions of pilocarpine and carbachol in salivary cells are mediated through two distinct signaling mechanisms via M3/M1 receptors.
- Data suggest that 77-LH-28-1 is a potent and selective agonist at recombinant human M1 mAChRs.
- analysis of a molecular that simultaneously utilizes both an allosteric and the orthosteric site on the M(2) mAChR