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Validated All-in-One™ qPCR Primer for PPARGC1A(NM_013261.4) Search again
Product ID:
HQP001016
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
LEM6, PGC-1(alpha), PGC-1alpha, PGC-1v, PGC1, PGC1A, PPARGC1
Gene Description:
PPARG coactivator 1 alpha
Target Gene Accession:
NM_013261.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism.
Gene References into function
- PRC is a functional relative of PGC-1 that operates through NRF-1 and possibly other activators in response to proliferative signals
- Mutation analysis. A widespread Gly482Ser polymorphism of PGC-1 is associated with a 1.34 genotype relative risk of Type II diabetes mellitus
- Peroxisome proliferator-activated receptor (PPAR) gamma coactivator-1 recruitment regulates PPAR subtype specificity.
- Egr-1 is necessary and sufficient to activate human PPARgamma1 gene expression in VSMC
- no association with type II diabetes and G482S polymorphism was detected in moderate and morbidly obese subjects
- results of this study suggested that the PGC-1 gene might be implicated in the pathogenesis of Type II diabetes
- identification of a specific molecular repressor of the gene
- PGC-1 alpha serves as a co-activator for the liver X receptor (LXR) alpha, known to contribute to the regulation of cellular cholesterol homoeostasis
- role of LXXLL domain for ligand-dependent and ligand-independent interaction and coactivation of peroxisome proliferator-activated receptor gamma
- PGC-1 can induce the fatty acid oxidation enzyme MCAD (medium-chain acyl-coenzyme A dehydrogenase) in an ERRalpha-dependent manner
- Exercise induces dramatic transient increase in PGC-1alpha transcription and mRNA content in human skeletal muscle. PGC-1alpha may coordinate activation of metabolic genes in human muscle in response to exercise.
- Data describe the expression of peroxisome proliferator-activated receptor gamma (PPAR gamma) co-activator 1, PPAR gamma, insulin receptor substrate-1, glucose transporter isoform-4, and mitochondrial uncoupling protein-1 in adipose tissue.
- PPARGC1 can participate in blood pressure control, and sequence substitutions at its gene locus confer an increased risk of hypertension to a substantial proportion of men.
- results indicate that signaling via protein kinase B to forkhead transcription factor FKHR can account for the effect of insulin to regulate peroxisome proliferator-activated receptor-gamma coactivator-1 promoter activity via the insulin response sequenc
- data indicate that the Gly482Ser missense polymorphism in PGC-1 has metabolic consequences on lipid metabolism that could influence insulin secretion
- Decreased PGC1 expression may be responsible for decreased expression of NRF-dependent genes, leading to the metabolic disturbances characteristic of insulin resistance and DM.
- Down-regulation of peroxisome-proliferator activated receptor-gamma coactivator 1 is associated with local recurrence and metastasis in breast cancer
- in human skeletal muscle, PGC-1alpha mRNA increased more after exercise with restricted blood flow than in the nonrestricted condition.
- PGC-1alpha has a fundamental role in activating CYP7A1 and bile acid biosynthesis
- Polymorphism associated with insulin resistance affects insulin snssitivity by interacting with PC-1 polymorphism
- Increases in PGC-1 and PPAR-alpha levels may play an important role in changes in muscle mitochondria content, oxidative phenotype, and sensitivity to insulin induced by endurance training.
- Examines whether common polymorphism (Gly482Ser) within the gene modifies the association between physical activity energy expenditure and VO2max
- our data suggest that the transcriptional activity of PPARgamma may not only be decreased by mutation but also by downregulation of the coactivator PGC-1 of PPARgamma.
- an interdependent ERRalpha/PGC-1alpha-based transcriptional pathway targets the ESRRA23 element to dictate ERRalpha expression level; regulatory polymorphism studied may provide differential responses to ERRalpha/PGC-1alpha-mediated metabolic cues
- Borderline significant differences of four-loci haplotype distributions in downstream haplotype block. Haplotype associated with strongest insulin response to glucose conferred lowest risk of type 2 diabetes.
- Gly482Ser variant in PGC-1 is not associated with diabetes-related traits or skeletal muscle fiber type composition.
- both PPARgamma and its coactivator PGC-1 play important roles in the development and progression of breast cancer
- ligand-activated PXR interferes with HNF-4 signaling by targeting the common coactivator PGC-1, which underlies physiologically relevant inhibitory cross-talk between drug metabolism and cholesterol/glucose metabolism
- PGC-1alpha functions as a potent coactivator for FXR and further implicates its role in the regulation of genes that are involved in bile acid and lipid metabolism.
- study of the crystal structure of ERRalpha ligand binding domain in complex with peroxisome proliferator-activated receptor coactivator-1alpha
- Gly482Ser polymorphism of the PGC-1 gene should be considered as a risk factor for the development of type 2 diabetes in Caucasians.
- 482Ser allele of the PGC-1alpha gene is associated with the conversion from impaired glucose tolerance to type 2 diabetes in the STOP-NIDDM trial.
- sex-specific association between Gly482Ser polymorphism and arterial hypertension in type 2 diabetic men.
- summary, our data indicate a role for the Gly482Ser genotype in determining aerobic fitness.
- Ser allele of PGC-1alpha Gly482Ser confers a significantly reduced risk of hypertension in whites.
- Data demonstrate that the multi-hormone response element of the estrogen-related receptor-alpha (ERRalpha) gene is a target for ERRgamma transactivation, which is enhanced by peroxisome proliferator-activated receptor-gamma coactivator-1alpha.
- two variations in the PGC-1alpha gene might not contribute to the risk of hypertension and type 2 diabetes in the Chinese population studied here
- Enhanced insulin-stimulated glycogen synthesis in human skeletal muscle cell culture coincides with increased PGC1 mRNA expression following treatment with various antidiabetic agents.
- PGC-1 alpha serves as a coactivator for the vitamin D receptor.
- PPARGC1A is likely to contribute to the risk of diabetes in offspring of patients with type 2 diabetes.
- PPARGC1A promoter polymorphisms are associated with age at diagnosis of type 2 diabetes and early-onset type 2 diabetes in the Korean population.
- PGC-1alpha coactivator activity is potentiated by arginine methylation by protein arginine methyltransferase 1 (PRMT1), another nuclear receptor coactivator.
- Coactivator peroxisome proliferator-activated receptor-gamma coactivator-1alpha is capable of stimulating the HNF-4alpha-dependent transactivation of apoAV promoter.
- The study suggested that the PGC-1alpha gene might be implicated in the pathogenesis of T2DM. But the studied SNPs in PGC-1alpha gene may not be major susceptibility ones of T2DM mellitus in Han people of Shanghai.
- The peroxisome proliferator-activated receptor-gamma coactivator-1alpha enhanced the GR/dexamethasone activation of the hNTCP promoter.
- fatty acids differentially regulated expression of the genes encoding the PGC-1A isoform
- A single nucleotide in an ERRalpha binding site can determine specific configuration to the receptor and productive interaction with the coactivator PGC-1alpha.
- PGC-1alpha gene expression is down-regulated by Akt- mediated phosphorylation and nuclear exclusion of FoxO1 in insulin-stimulated skeletal muscle
- Data suggest that the Gly482 Ser variant of the peroxisome proliferator-activated receptor gamma coactivator-1alpha gene may be an independent genetic risk factor for young-onset hypertension.
- Expression in differentiated human skeletal muscle cells is induced by unsaturated non-esterifed fatty acids.
- Significant association found between the Gly482Ser missense mutation of the PGC-1alpha gene and reduced insulin sensitivity in obese subjects indicates a primary role for PGC-1alpha in genetic susceptibility to insulin resistance.
- Meta-analysis: a modest role for the Gly482Ser PPARGC1A variant in type 2 diabetes risk
- The risk of obesity associated with 482Ser is evident only in physically inactive elderly male subjects
- The results obtained using truncated PGC-1alpha proteins suggested that two regions are necessary for PGC-1alpha to interact with the DNA-binding complex of RXRalpha/FXR.
- The polymorphisms of the peroxisome proliferator-activated receptor-gamma (Pro12Ala) and its coactivator-1 (Gly482Ser) genes were investigated among 201 Chinese Han women with polycystic ovary syndrome (PCOS).
- These results suggest that PGC-1alpha variants with Gly/Gly at 482nd amino acid may impair the Tfam transcription, a regulatory function of mitochondrial biogenesis, resulting in dysfunctional mtDNA replication.
- PPARGC1A, PPARGC1B, and EP300 may have roles for familial breast cancer susceptibility
- Defects in PGC-1alpha expression and regulation may contribute to the pathophysiology of type 2 diabetes in humans.
- The PGC-1alpha protein is directly acetylated by GCN5 resulting in a transcriptionally inactive protein that relocalizes from promoter regions to nuclear foci.
- Single nucleotide polymorphisms in PPARD modify the conversion from glucose intolerance to type 2 diabetes particularly in combination with polymorphisms in PGC-1A.
- CYP2A6 is induced via PXR and PGC-1alpha through the DR4-like element at the distal response region.
- These results establish the importance of coactivators PGC1alpha and SRC1 for the hepatic expression of human P450s and uncover a new HNF4alpha-dependent regulatory mechanism to constitutively control the CYP1A1/2 cluster.
- A key coactivator for the proper function of HNF4alpha in human liver and for an integrative control of multiple hepatic genes involved in metabolism and homeostasis.
- Nitric oxide can regulate the mitochondrial reactive oxygen species detoxification system both positively and negatively through PGC-1alpha.
- Acute elevation of plasma non-esterified fatty acd levels downregulates PPARGC1A, PPARGC1B and PPARA expression.
- Risk factors for distinct carotid phenotypes may vary and suggest, but do not prove, that PGC-1alpha may contribute to the regulation of atherogenic pathways.
- the induction of apoA-IV expression in fasting and diabetes likely involves PGC-1 alpha-mediated coactivation of HNF-4 alpha in addition to glucocorticoid-dependent actions
- Data show that mutant huntingtin causes disruption of mitochondrial function by inhibiting expression of PGC-1alpha.
- Recent studies have elucidated the function of the PGC-1 coactivators in different tissues and have highlighted the implications of PGC-1 dysregulation in diseases such as diabetes, obesity, cardiomyopathy, or neurodegeneration.
- PGC-1alpha is involved in the RNA processing of TRalpha transcripts.
- Its regulatory function in lipid metabolism makes it an inviting target for pharmacological intervention in the treatment of obesity and Type 2 diabetes. (Review)
- These findings suggest that PGC-1alpha elevates the binding of GR to Palin and thereby enhances the GR-mediated inhibition of human insulin transcription.
- This is the first study implicating that the Gly482Ser and Thr612Met polymorphisms in PGC-1alpha and Pro12Ala polymorphism in PPARgamma2 do not affect the functional integrity of these proteins.
- Non-esterified fatty acid clearance is blunted following a glucose load in carriers of the PPARCG1A Ser482 allele, and is associated with obesity.
- Thr394Thr SNP in the PPARGC1 gene was associated with peak BMD in the femoral neck in Chinese women.
- deletion of the activation domains (AD) did not eliminate ability of PGC-1alpha to interact with thyroid hormone receptor. data indicate that AD1 & AD2 motifs mediate induction of many PGC-1- responsive genes.
- PPAR gamma mRNA was significantly higher in normal colon tissue compared with tumor tissue.
- The activated proinflammatory state of monocytes and MDM in low HDL-C subjects constitutes a novel parameter of risk associated with HDL deficiency, related to altered expression of metallothionein genes and the reciprocal regulation of PPARgamma.
- the minor G allele of PPARD was associated with less increase in individual anaerobic threshold during lifestyle intervention
- We therefore suggest that this PPARGC1A allele may play a role in the large difference in type II diabetes prevalence between Polynesians and neighboring populations.
- PGC-1alpha was involved in the apoptotic signal transduction pathways and downregulation of PGC-1alpha may be a key point in promoting epithelial ovarian cancer growth and progression.
- Expression of peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha), known to be upregulated in the liver by fasting, was found to abolish the cAMP-dependent downregulation of glucagon receptor mRNA expression in vitro.
- Mitochondrial genotype observed in association with Type 2 Diabetes Mellitus in combination with PGC1A variants showed an increased prevalence in controls with mt10398G and 16189T along with G/G genotype background at the two polymorphic loci of PGC1A.
- Our results indicate an essential role for ERRalpha as a key regulator of oxidative metabolism.
- No association between this gene and type 2 diabetes or body mass index in a Hispanic and non-Hispanc population in Colorado.
- results do not support previous data on Caucasians showing an association between the Gly482Ser variant in PPARGC1A and VO2max but suggest the potential role of another polymorphism (A2962G) to explain individual VO2max differences in Chinese men.
- Review. PGC-1a is a master regulator of myocardial energy metabolism in diverse physiological and pathophysiological conditions.
- No association was found between PGC-1alpha polymorphism and hypertension with or without LVH; data indicate that variants of the PGC-1alpha gene are correlated with increased risk for HCM.
- Novel pathways regulated by PGC-1 alpha reveal that PPARalpha controls gene expression in human white adipocytes.
- The 482G/A variant could decrease binding force between PGC-1alpha and MEF2C and increase the risk of type 2 diabetes in Chinese Han population by PGC-1alpha -MEF2C-GLUT-4 pathway.
- comparison of risk genotype combinations of: UCP2-866GG, mtDNA 10398A and PGC1alpha p.Thr394Thr or p.Gly482Ser for NIDDM
- Weight loss upregulates PGC1A, which in turn stimulates mitofusin-2 expression, which contributes to the improvement of insulin sensitivity.
- Improvement and restoration of mitochondrial function and oxidative capacity through activation of PGC-1 alpha could provide new treatments for metabolic diseases, as suggested by the evidence in this review.
- G482S and +2962A/G polymorphisms of PGC-1alpha gene were significantly associated with only severe hypertension in 1642 apparently healthy Chinese residents.
- Polymorphisms of PGC-1alpha gene were only significantly associated with severe hypertension defined by occasional clinic blood pressure measurements.
- The results suggested that the 482Ser variant of PGC-1alpha conferred the susceptibility to type 2 diabetes in the southern Chinese population.
- PPARGC1A variations influence development of type 2 diabetes and cardiovascular diseases via DNA damage.
- Increased histone acetylation might be responsible for PGC-1alpha-mediated transactivation of a minimal E-cadherin promoter.
- After downregulation of PPARGC1A expression in human islets by small interfering RNA, insulin secretion was reduced by 41%.
- Data show that PPARGC1A is a susceptibility gene for type 2 diabetes in Chinese Han population in Hubei.
- PGC-1alpha is an important co-activator for LRH-1 and that SHP targets the interaction between LRH-1 and PGC-1alpha to inhibit CYP7A1 expression.
- providing a plausible explanation for distinct PGC-1alpha binding to ERRalpha monomers and homodimers
- Meta-analysis suggests that the PPARGC1A Ser482 allele may be associated with higher blood pressure, but this is only apparent in younger adults.
- the strong interaction of PGC-1alpha and PPARgamma is mediated through both hydrophobic and specific polar interactions.
- PPARGC1A up-regulates APLN protein in human adipocytes.
- May be important in the human glucose-6-phosphatase catalytic subunit gene promoters activation.
- PPARGC1A polymorphism and lower expression of PPARGC1A mRNA in the liver are an important genetic contribution to etiology of nonalcoholic fatty liver disease.
- The observed gender-specific association between PGC-1alpha and the plasma adiponectin level may reflect linkage disequilibrium of Gly482Ser polymorphism with other causative variations.
- Examine rapid exercise-induced changes in PGC-1alpha mRNA and protein in human skeletal muscle.
- Results describe the mapping of the nucleosome positions of the estrogen-related receptor alpha gene promoter and examine the changes of histone acetylation in response to peroxisome proliferator-activated receptor-gamma coactivator-1alpha expression.
- DJ-1 stimulates the activity of a master regulator of mitochondrial biogenesis and stress response, peroxisome proliferator-activated receptor-gamma co-activator 1alpha (PGC-1alpha), in the transcription of the MnSOD
- No differences in mRNA levels of genes which mediate the transcriptional of mitochondrial biogenesis (PPARGC1A and NRF1) or in mitochondrial mass between diabetic and control myotubes.
- Data show that GW501516 and activation of PPARbeta/delta stimulate human lung carcinoma cell proliferation, and that GW501516 stimulates PGC-1alpha, which activates the PI-3 kinase/Akt mitogenic pathway.
- PPAR-alpha and PGC-1A polymorphisms are associated with alcohol consumption in the Mediterranean population
- The present review examines the evidence in support of the key role for PGC-1alpha regulation of substrate metabolism and mitochondrial biogenesis in skeletal muscle--REVIEW
- A novel AMPK-mediated regulatory pathway that regulates PGC-1alpha gene expression.
- the presence of the Ala allele at the PPARG/Pro12Ala polymorphism and the Ser allele at the PPARGC1A/Gly482Ser polymorphism may be a predisposing factor for sarcoidosis.
- These results suggest that PGC-1alpha regulates multiple pathways in neurons and that HDACi's may be good candidates to target PGC-1alpha and GLUT4 in Huntington's Disease and other neurological disorders.
- PGC-1alpha-mediated inhibition of vascular smooth muscle cells proliferation
- PGC-1alpha is coupled to HIF-1alpha-dependent gene expression by increasing mitochondrial oxygen consumption in skeletal muscle cells