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Validated All-in-One™ qPCR Primer for NES(NM_006617.1) Search again
Product ID:
HQP000899
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
Nbla00170
Gene Description:
nestin
Target Gene Accession:
NM_006617.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM].
Gene References into function
- Nestin-containing neurons occurred in hippocampus, septum, diagonal band, amygdala and basal nucleus of Meynert. Nestin-containing astrocytes occurred in the subependymal zone and dentate gyrus.
- The tissue distribution of nestin has been studied in the muscularis propria of normal gastrointestinal autopsy material.
- Beta-cell differentiation during human development does not rely on nestin-positive precursors
- Nestin-immunoreactive cells colocalized with vimentin throughout the retina. Colocalized with mitotic Ki67 immunoreactive cells.
- Demonstration of strong expression of nestin in Purkinje cells in pathologically advanced Creutzfeldt-Jakob disease correlates with the process that Purkinje cells undergo to promote survival.
- detection of nestin-positive keratinocytes in the basal layer of cultured epidermis
- nestin may be one mediator of N-myc-associated tumor aggressiveness of human neuroblastoma
- In transgenic mice, nestin transgene expression in the olfactory bulb is limited to periglomerular neurons and is absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations.
- Nestin expression in tumor endothelium is enhanced by the first intron.
- nestin has a role in multi-potentiality and regenerative potential [review]
- nestin expression is recognized under all conditions of vascular development
- Nestin is constantly expressed in situ in the cortex of normal human adrenal glands; a positive nestin immunoreaction could be useful in differential diagnosis of clear cell tumors in adrenal glands.
- Data evaluate and compare the bcl2, bax, and nestin patterns in the frontal cortices of Alzheimer and multiple-infarct dementia patients, and in normal aging.
- These results show that the pattern of nestin expression is related to the differentiation of epidermal stem cells.
- Moderate to strong expression of nestin was observed in all tumors.
- Nestin expression in adenomas cannot be viewed as a biologically relevant marker of cell proliferation and as a prognostic indicator in pituitary neoplasms.
- Purkinje cells were immunoreactive with nestin regardless of the severity of degenerative cerebellar cortex.
- cultured pancreatic islets contain nestin and Isl-1 positive mesenchymal stem cells with multipotential developmental capacity
- In a series of 40 surgical specimens, including gliomas, vascular malformations, abscesses and angiomas, nestin was poorly expressed in mature astrocytes and more expressed in developing reactive astrocytes, mainly in the cytoplasm.
- The putative pancreatic stem cells expressed pdx-1 and nestin.
- nestin is a survival determinant whose action is based upon a novel mode of Cdk5 regulation, affecting the targeting, activity, and turnover of the Cdk5/p35 signaling complex
- Nestin is expressed in the human kidney from the first steps of glomerulogenesis within podocytes, mesangial, and endothelial cells.
- The expression of nestin in the podocytes of normal and diseased kidneys of adolesscent and adult patients is reported.
- nestin has a role in neovasculaturization in colorectal cancer
- Nestin is more sensitive for malignant peripheral nerve sheath tumor (MPNST) than other neural markers, and immunostains for nestin in combination with other markers could be useful in the diagnosis of MPNST.
- Transgenic mice in which the nestin neural enhancer drives expression of a green fluorescent protein reporter show that the regulation in Schwann cells is transcriptional.
- The nestin(-) vimentin(+) fibroblasts may represent a novel type of multipotent adult stem cells in human dermis.
- Nestin is expressed in podocytes of mature human glomerular tissues in quiescent states.
- The extent of nestin-positive neuronal cells correlated with the appearance of tyrosine hydroxylase positive neurons.
- Results specify a function for Nestin in cell motility and identify a novel pathway for prostate cancer metastasis.
- enhanced expression of nestin in the injured myocardium might reflect spontaneous regenerative processes supposedly based on the differentiation of resident cardiac stem cells into diverse cardiac cell types.
- Nestin is expressed in proliferating and metabolically active cells, such as endothelium, independent of developmental and neoplastic processes.
- Nestin plays a crucial role in remodeling the cytoskeleton of cells in the human postinfarcted myocardium.
- this study provided the first evidence that nestin was used as a new differentiation marker of endothelial progenitor cells
- Dental follicle cells expressed the stem cell marker nestin in both serum-free and serum-containing cell culture media.
- Cyclosporin A induced renal injury recruits nestin-expressing cells to injured areas.
- Nestin expression in pancreatic cancer cells may contribute to nerve and stromal invasion in this malignancy