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Validated All-in-One™ qPCR Primer for GNA13(NM_006572.5) Search again
Product ID:
HQP000820
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
G13, HG1N
Gene Description:
G protein subunit alpha 13
Target Gene Accession:
NM_006572.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin
- Galpha13 is not associated with lipid rafts
- Galpha13 can induce ppET-1 gene expression through a JNK-mediated pathway.
- co-stimulation of G(12/13) and G(i) pathways is sufficient to activate GPIIb/IIIa in human platelets in a mechanism that involves intracellular calcium
- These results suggest that protein kinase A blocks Rho activation by phosphorylation of Galpha(13) Thr(203).
- Selective activation of Galpha(12) and Galpha(13) by thrombin and LPA, respectively, is determined by the N-terminal short sequences of alpha subunits.
- Several features of a typical alpha/RGS interaction are preserved in the alpha(13)/p115RhoGEF interaction.
- Galpha13-induced VASP phosphorylation that involves activation of RhoA and MEKK1, phosphorylation and degradation of IkappaB, release of PKA catalytic subunit from the complex with IkappaB and NF-kappaB, and subsequent phosphorylation of VASP
- analysis of the molecular mechanism of how the human TXA2 receptor interacts with G alpha 13 to activate intracellular signaling
- Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration.
- Results identify the G12 family proteins Galpha12 and 13 as important regulators of prostate cancer invasion and suggest that these proteins may be targeted to limit invasion- and metastasis-induced prostate cancer patient mortality.
- Galpha13-Rho signaling axis is required for SDF-1-induced migration through CXCR4
- Distinct regions of Galpha13 participate in its regulatory interactions with RGS homology domain-containing RhoGEFs.
- analysis of a novel cross-talk exerted from the LPA/Galpha(13)/p115RhoGEF/RhoA pathway to the beta(2)-adrenergic receptor/Galpha(s)/adenylyl cyclase pathway
- G alpha(12/13) regulate basal p53 levels via mdm4, which constitutes a cell signaling pathway distinct from p53 phosphorylations elicited by genotoxic stress.
- role in regulating ASK1 expression levels
- provides first examination of Galpha12 and Galpha13 in the human heart, demonstrating selective activation of human atrial Galpha12 and Galpha13 by endothelin and angiotensin receptors, respectively
- A pronounced and rapid translocation of p115-RhoGEF from the cytosol to the plasma membrane was observed upon activation of several G(12/13)-coupled receptors in a cell type-independent fashion.
- AC7 is a specific downstream effector of the G(12/13) pathway
- RGS22 may also play a role in GNA13 translocation from the cytoplasm to the nucleus during spermiogenesis
- Protein kinase C-related kinase and ROCK are required for thrombin-induced endothelial cell permeability downstream from Galpha12/13 and Galpha11/q
- G(alpha)(13)-dependent downstream effects on RhoA activation and invasion tightly depend on cell type-specific GAP activities and that G(alpha)(13)-p190RhoGAP signaling might represent a potential target for intervention in melanoma metastasis.