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Validated All-in-One™ qPCR Primer for IGF2BP3(NM_006547.2) Search again
Product ID:
HQP000778
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CT98, IMP-3, IMP3, KOC, KOC1, VICKZ3
Gene Description:
insulin like growth factor 2 mRNA binding protein 3
Target Gene Accession:
NM_006547.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq].
Gene References into function
- The growth regulating gene IMP-3 has been characterized as a candidate for Silver-Russell syndrome.
- KOC is a sensitive and specific marker for carcinomas and high-grade dysplastic lesions of the pancreatic ductal epithelium
- IMP-3 knock down by RNA interference significantly decreases levels of intracellular and secreted insulin-like growth factor-II (IGF-II) without affecting IGF-II leader-3, leader-4, c-myc, or beta-actin mRNA levels and H19 RNA levels.
- We conclude that in patients with Barrett's esophagus, GOAGE for IGF-II is found frequently in the metaplastic esophageal epithelium as well as in normal gastric and duodenal epithelia.
- Adenocarcinoma in situ of the uterine cervix demonstrate significant expression of IMP3.
- VICKZ exhibits differential expression in lymphoma subtypes and thus may be a marker of potential value in the diagnosis and study of hematopoietic neoplasia.
- study shows KOC is highly expressed in high-grade lung neuroendocrine carcinomas but not low- & intermediate-grade carcinoid tumors, indicating KOC may play an important role in regulation of biologic behavior of high-grade lung neuroendocrine carcinomas
- Immunohistochemical detection of KOC expression may be a useful diagnostic tool for distinguishing between small cell carcinoma and carcinoid tumor.
- Expression of IMP3 and p53 may be helpful biomarkers in the distinction of endometrial serous carcinoma from endometrioid adenocarcinoma
- IMP-3 appears to be involved in the progression of malignant melanoma
- IMP3 may be a useful diagnostic marker in the assessment of endometrial cancers and their precursor lesions.
- IMP3 over expression was associated with metastatic progression and death for patients with clear cell renal cell carcinoma
- Rab27A acts as a novel mediator of invasion and metastasis promotion in human breast cancer cells through regulating the secretion of IGF-II.
- IMP3 is an independent prognostic marker that can identify a group of patients with a high potential to develop progression.
- IMP3 is a promising new marker with high specificity and sensitivity for pancreatic adenocarcinoma.
- The concurrent use of KOC and S100A4 protein improves the diagnostic sensitivity of biliary brushings cytology.
- IMP3 plays an important role in tumor invasion and metastasis and is a strong prognostic factor for patients with hepatocellular carcinoma.
- We propose K homology domain-containing protein overexpressed in cancer as a potential target molecule for the treatment of high-grade neuroendocrine carcinomas.
- IMP3 expression was correlated with tumor metastasis in osteosarcoma.