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Validated All-in-One™ qPCR Primer for IGF2BP1(NM_006546.3) Search again
Product ID:
HQP000777
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1
Gene Description:
insulin like growth factor 2 mRNA binding protein 1
Target Gene Accession:
NM_006546.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. [provided by RefSeq].
Gene References into function
- IMP1 localization is associated with motility and the major functions of IMP1 are carried out by the phylogenetically conserved KH domains
- Aberrant CRD-BP expression may interfere with c-myc regulation. Copy number gains at 8q24 (c-myc), were seen in 48.3% of tumors, gains at 17q21 (CRD-BP) in 18.3%,& CRD-BP was seen in 58.5%, implying mechanisms of activation other than gene amplification.
- IMP1 translocates to the nucleus and contains nuclear export signals within the RNA-binding KH2 and KH4 domains.
- IMP-1 may have a role in progression of ovarian cancer
- Intestinal epithelial cells continue to express IMP1 postnatally, and Imp1(-/-) mice exhibited impaired development of the intestine, with small and misshapen villi and twisted colon crypts.
- highest frequency of CRD-BP positive tumors was observed in meningiomas
- intimate association of fragile X mental retardation protein and IMP1 suggests a link between mRNA transport and translational repression in mammalian cells
- A cooperative mechanism for the binding of IMP-1 to RNA.
- CRD-BP has a dominant role in proliferation of human K562 cells by an IGF-II-dependent mechanism independent of its ability to serve as a c-myc mRNA masking protein
- normal role for CRD-BP/IMP1 in pluripotent stem cells with high renewal capacity
- We have further characterized the interaction between PABP and IMP1 with the 3' end of the adenine-rich autoregulatory sequence
- IMP-1 expression is likely to play important roles in lung cancer development and progression
- Study isolated the IMP1-containing RNP granules and found that they represent a unique ribonucleoprotein entity distinct from neuronal hStaufen and/or fragile X mental retardation protein granules, processing bodies, and stress granules.
- VICKZ exhibits differential expression in lymphoma subtypes and thus may be a marker of potential value in the diagnosis and study of hematopoietic neoplasia.
- IMP1 is an oncogenic factor that is involved in promoting elevated proliferation by stabilizing the c-myc mRNA in ovarian carcinoma cells.
- IMP-1 associates with Gag protein of HIV-1 and its overexpression affects virus assembly.
- Knockdown of CRD-BP inhibits NF-kappaB activity, induces apoptosis, and suppresses proliferation and tumorigenic properties of melanoma cells.
- These experiments suggest that in breast cancer cells, the expression of ZBP1 and the expression of beta-catenin are coordinately regulated.