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Validated All-in-One™ qPCR Primer for CXCL13(NM_006419.2) Search again
Product ID:
HQP000672
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
Gene Description:
C-X-C motif chemokine ligand 13
Target Gene Accession:
NM_006419.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq].
Gene References into function
- The effect of surrogate antigen on B-cell chemotaxis response to BCA-1 is biphasic: After an initial phase of suppression, chemotaxis toward BCA-1 is strongly up-regulated.
- expression by malignant B-lymphocytes and vascular endothelium in primary central nervous system lymphoma
- overlapping role with interleukin 7 recedptor alpha and CCR7 ligands in lymph node development
- CXCR5 and CXCL13 play an essential role in maintaining B- and T-cells in lymphocytic infiltrates and ectopic follicles in thyroid tissue from patients affected by autoimmunity.
- most CXCL13-expressing cells in rheumatoid arthritis and ulcerative colitis are of monocyte/macrophage lineage.
- Potenetial role of CXCL13 and its specific receptor CXCR5 in recruitment of B cells in renal allograft rejection.
- persistent production of CXCL13 and IgG within infected tissue, two characteristics of ectopic germinal centers, are definitive features of Lyme neuroborreliosis
- The data support expression of CXCL13 and CCL21 in regulating the progressive lymphoid organization and maintenance of periductal foci in Sjogrens syndrome.
- Data suggest that CXCL12 and CXCL13 may directly modulate cellular proliferation and collagen type I in osteoarthritis patients.
- elevated levels of CXCL13 could cause impaired or altered trafficking of B cells during HIV infection and could contribute to the previously reported loss of CXCR5
- a high CXCL13 production by epithelial cells could be responsible for germinal center formation in myasthenia gravis thymus
- CXCL13 and CCL19 cooperatively induce significant resistance to TNF-alpha-mediated apoptosis in acute and chronic B cell leukemia CD23+CD5+ B cells, but not in the cells from cord blood.
- CXCL13 promotes recruitment of B and T lymphocytes within the islets of Langerhans of transgenic mice.
- prostate-associated lymphoid aggregates, frequently below the epithelia, arranged in B cell follicles expressed CXCL13, and parafollicular T cell areas showed CCL21 expression.
- CXCL13 and CCL21 are expressed in ectopic lymphoid follicles in cutaneous lymphoproliferative disorders.
- Angioimmunoblastic T-cell lymphoma T cells localize in the skin allowing accurate diagnosis in skin specimens using CXCL13 immunostaining on paraffin-embedded tissues.
- CXCR5 plays a role in Chronic lymphocytic leukemia (CLL) cell positioning and cognate interactions between CLL and CXCL13-secreting CD68+ accessory cells in lymphoid tissues.
- CCL19 plus CXCL13 regulate interaction between B-ALL CD23+CD5+ B cells and CD8+ T cells by inducing activation of PEG10, in turn, resulting in IL-10 overexpression and impairment of tumor-specific cytotoxicity in syngeneic CD8+ T cells.
- knee synovial fluid levels of CXCL 13 were higher in Rheumatoid arthritis and Behcets disease patients compared to Osteoarthritis patients
- These findings indicate a previously unreported role for the BAFF/BAFF-R pair in mature B-cell chemotaxis.
- AITL is a morphologically heterogeneous T-cell lymphoma commonly expressing CXCL13 and CD10; high level of CD10 and CXCL13 did not affect survival.
- in salivary gland MALT lymphoma the lymphoid chemokines CXCL13 and CCL21 are directly implicated in the organization of ectopic reactive lymphoid tissue
- Expression of CXCL13 was found in cells of a dendritic-like morphology.
- up-regulation of CXCL13 gene expression is a feature of HCV-infected patients. Higher levels of this chemokine in liver as well as skin of patients with active mixed cryoglobulinemia vasculitis suggest a possible interrelation.
- report an effective method to establish human allo-reactive Th17 cell clones and demonstrate that human Th17, but not Th1 or Th2, cells express B cell chemoattractant CXCL13, by using DNA chips
- CCL23, M-CSF, TNFRSF9, TNF-alpha, and CXCL13 are predictive of rheumatoid arthritis disease activity and may be useful in the definition of disease subphenotypes and in the measurement of response to therapy in clinical studies.
- experiments performed to investigate the survival, adhesion, and metalloproteases secretion indicate that CXCL13 combined with CCL19 and CCL21 mainly affects the chemotaxis of Sezary syndrome cells
- Altered expression of the chemokine receptor-ligand pair, CXCR5/CXCL13, may participate in the establishment of B-cell dysfunctions during HIV-1 infection.
- CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients
- study demonstrates that CXCL13 is produced by dysplastic and neoplastic follicular dendritic cells and can be instrumental in recruiting intratumoural CXCR5+ lymphocytes
- The similar pattern of expression of CXCL13 and PD-1 in angioimmunoblastic T-cell lymphoma provides further evidence that AITL is a neoplasm derived from germinal-center T-helper cells.