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Validated All-in-One™ qPCR Primer for ARL6IP5(NM_006407.3) Search again
Powered by BiozBy default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Expression of this gene is affected by vitamin A. The encoded protein of this gene may be associated with the cytoskeleton. A similar protein in rats may play a role in the regulation of cell differentiation. The rat protein binds and inhibits the cell membrane glutamate transporter EAAC1. The expression of the rat gene is upregulated by retinoic acid, which results in a specific reduction in EAAC1-mediated glutamate transport. [provided by RefSeq].
Gene References into function
- highly conserved protein and genomic organization amongst vertebrates
- protein expression is upregulated by methyl-beta-cyclodextrin and not by retinoic acid
- Rsults suggest that JWA can be regulated by oxidative stress and is actively involved in the signal pathways of oxidative stress in the cells.
- Data show that the JWA -76G-->C variant genotype may play an important role in transcription regulation of JWA gene and in the susceptibility to bladder cancer.
- JWA may function as a lineage-restricted gene during differentiation along the monocyte/macrophage-like or granulocytic pathway
- all-trans retinoic acid up-regulates JWA expression by stimulating the transcriptional activity of JWA gene promoter
- This paper deals primarily with PRAF2, but comparisons with PRAF3 are also provided.
- JWA participates in the signal pathways of H2O2 induced oxidative stress in K562 cells
- The effects of All Trans Retinoic Acid in regulating cellular proliferation and apoptosis may be mediated in part by JWA expression.
- JWA regulated-tumor cellular migration might involve MAPK cascades activation and F-actin cytoskeleton rearrangement mechanisms.
- Three novel functional genetic poly- morphisms of JWA gene, -76C, 454A, and 723G, appear to contribute to the etiology of bladder cancer
- Single nucleotide polymorphisms of JWA were associated with enhanced risk of gastric cancer and esophageal squamous cell carcinoma in a Chinese population.
- The potentially functional genetic polymorphism 454CA of the JWA gene appears to contribute to the risk of multiple kinds of leukemia in a south Chinese population.
- These results show GTRAP3-18 to negatively and dominantly regulate cellular GSH content via interaction with EAAC1 at the plasma membrane.
- Expression of GTRAP3-18 delays the ER exit of EAAC1, as well as other members of the excitatory amino acid transporter family.