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Validated All-in-One™ qPCR Primer for CEBPB(NM_005194.3) Search again
Product ID:
HQP000623
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C/EBP-beta, IL6DBP, NF-IL6, TCF5
Gene Description:
CCAAT enhancer binding protein beta
Target Gene Accession:
NM_005194.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq].
Gene References into function
- The activities of several functional C/EBPbeta responsive elements in the promoter region and in the intron of CCR5 suggest regulatory roles for C/EBPbeta in CCR5 gene expression and in the pathogenesis of HIV disease.
- These findings provide new insight into the regulation of cyclooxygenase-2 promoter by an interplay between two opposite beta isoforms and p300 co-activator.
- PGE(2) induces HIV-1 LTR activity in t cells through a complex interplay between C/EBPbeta and CREB
- CCAAT/enhancer binding protein-beta is a mediator ofkeratinocyte survival and skin tumorigenesis involving oncogenic Ras signaling.
- C/EBP beta binding to the C/EBP site, which is in part mediated via activation of RSK, can primarily explain the TPA responsiveness of the IGF-I gene promoter
- cooperation with cyclic AMP-induced forkhead transcription factor in differentiating human endometrial stromal cells
- Conserved amino acids regulationregulate phosphoenolpyruvate carboxykinase (PEPCK) gene expression.
- Conditional expression of C/EBP alpha induced the C/EBP family members C/EBP beta and C/EBP epsilon and subsequent granulocyte differentiation.
- Decreased activity and enhanced nuclear export of CCAAT-enhancer-binding protein beta during inhibition of adipogenesis by ceramide.
- Organization of the promoter regions involved in transcriptional activation of the human NF-IL6 gene
- both phosphorylation and dephosphorylation of C/EBPbeta in response to growth hormone coordinately modulate its ability to activate transcription by modulating its DNA binding activity and its transactivation capacity
- Calreticulin interacts with C/EBPalpha and C/EBPbeta mRNAs and represses translation of C/EBP proteins.
- Regulates HIV-1 transcription in activated Jurkat T cells
- IL-1 induces CRP expression through 2 overlapping response elements, the binding sites for CCAAT-box/enhancer-binding protein-beta (C/EBP-beta) and p50-nuclear factor-kappaB (p50-NFkappaB).
- 5'-region from -252 to -175, containing a consensus site for CCAAT/enhancer binding proteins alpha,beta (C/EBPalpha,beta), was essential for SAA1 induction in HASMCs.
- IRF-1-CEBPbeta complex activate the promoter of IL-18 binding protein.
- the level of basal as well as cAMP-stimulated IL-10 transcription depends on the expression of C/EBP alpha and beta and their binding to three motifs in the promoter/enhancer region
- A C/EBP beta element in the FasL promoter, identified by a functionally significant single-nucleotide polymorphism, affects FasL expression in vitro and ex vivo and is associated with systemic lupus erythematosus in African American patients.
- MEK2 and p38 in IFN-gamma-mediated signal transduction and induction of C/EBP beta expression and activity associated with interleukin-6 (IL-6) secretion in colon epithelial cells.
- Positively functioning NF-IL6 (C/EBPbeta)is not constitutively multiubiquinated by the proteasome. Deletion of leucine zipper domain in NF-IL6 caused the loss of its homodimerization activity & its degradation by the ubiquitin-proteasome system.
- C/EBP-beta may have a role in promoting tumor invasiveness
- HMGI-Y physically interacts with Sp1 and C/EBP beta and facilitates the binding of both factors to the insulin receptor promoter
- There are high levels of FGF-BP expression in invasive human breast cancer, relative to normal breast and in situ carcinoma, and in MDA-MB-468 human breast cancer cells.
- Papillomavirus E2 protein binds to C/EBP factors and may contribute to enhancing keratinocyte differentiation, which is suppressed by the viral oncoproteins E6 and E7 in HPV-induced lesions
- Pretreatment of monocytic cells with low TNF doses inhibited TNF-induced (restimulation with a high dose) IL-8 promoter-dependent transcription as well as IL-8 production, which was prevented by overexpression of C/EBPbeta, but not p65 or Oct-1.
- transcriptional activation of the interleukin-8 promoter by bradykinin involves the prostanoid-independent activation of nuclear factor-kappaB, and prostanoid-dependent activation of activating protein-1 and nuclear factor-interleukin-6
- Expression levels of C/EBPbeta isoforms in breast tumors were correlated with clinicopathological tumor parameters, expression of estrogen and progesterone receptors (ER, PR), Ki67 immunostaining, and expression of 7 cell-cycle regulatory proteins.
- These results suggest that CSF-induced and HIV-1-mediated regulation of Hck and C/EBPbeta represent the heterogeneous susceptibility of tissue macrophages to HIV-1 infection.
- study provides strong evidence that early growth response 1 regulates low density lipoprotein receptor transcription via a novel mechanism of protein-protein interaction with CCAAT enhancer-binding protein beta
- We conclude that C/EBPalpha and C/EBPbeta contribute to the deregulated expression of Bcl-2 in t(14;18) lymphoma cells
- isoform LAP* is the primary isoform of C/EBP beta that regulates, through a redox switch, the LPS-induced expression of the IL-6 gene.
- Overexpression of C/EBP beta was sufficient to increase basal expression of a Dlx3 reporter gene in a dose-dependent manner.
- Transfection of NF-IL63'UTR nduced tumor suppression in a human hepatoma cell line.
- Calcitrio may signal cell differentiation of HL 60 cells which in turn regulates expresson of CEBPB
- Ras-induced structural alteration of C/EBPbeta determines differential gene activation through selective interaction with distinct Mediator complexes
- EBPbeta could be an important gene in acute promyelocytic leukemia pathogenesis
- mechanisms of MDR1 activation by C/EBPbeta include C/EBPbeta binding of the chromatin of the MDR1 gene and interactions of C/EBPbeta with the Y box and Y box-associated proteins.
- Data show that epidermal growth factor receptor signaling results in phosphorylation of CUG-BP1, and leads to increased binding of CUG-BP1 to CCAAT/enhancer binding protein beta (C/EBP beta) mRNA and elevated expression of the C/EBPbeta LIP isoform.
- CCAAT/enhancer-binding protein beta gene expression is activated by endoplasmic reticulum stress through an unfolded protein response element downstream of the protein coding sequence
- The specific expression pattern of C/EBP-beta in gestational trophoblastic disease indicate that C/EBP-beta could potentially be an additional marker of such lesions.
- ATF3-FL and C/EBPbeta act as transcriptional suppressors for the ASNS gene to counterbalance the transcription rate activated by ATF4 following amino acid deprivation
- NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages
- Direct interaction between the IL6 promoter and the C/EBP beta protein in myxoid lipoarcoma.
- C/EBP beta is identified as a new partner for HIV-1 Tat in stimulating monocyte chemoattractant protein 1 (MCP-1) transcription in astrocytes.
- Defective thermoregulation owing to the lack of C/EBPbeta is associated with the reduced capacity to supply fatty acids as fuels to sustain brown fat thermogenesis.
- C/EBPbeta is involved in the down-regulating of trefoil factor 1 (TFF1) gene expression.
- Activation by C/EBP alpha and beta did not depend on their binding to the C/EBP site, since they still activated IGFBP-5 promoter.
- A new distal enhancer site is identified in the CYP3A4 gene where C/EBPbeta-LAP binds and activates transcription, whereas the truncated form, C/EBPbeta-LIP (liver inhibitory protein), antagonizes LAP activity and causes gene repression.
- This factor plays important roles in the regulation of thyroid specific genes and processes, and its functions are altered in human thyroid carcinoma.
- Transactivators C/EPB-beta and GATE-binding factor 1 (GBF1) physically interact to induce interferon-gamma-regulated transcription; an intact GTPS motif in C/EBP beta regulatory domain 2 is required for an interaction with GBF1.
- Together this shows that glucocorticoids increase DNA-binding activity of C/EBPbeta via post-translational mechanism(s) involving phosphorylation.
- findings show that expression of C/EBPbeta, which contributes to the regulation of amino-acid-responsive genes, is itself controlled by amino acid availability through transcription
- negative cross-talk between p53 and C/EBPbeta is likely to impact expression of their respective target genes
- C/EBPbeta is involved in enhancing transcription from the P670 promoter of HPV16 during keratinocyte differentiation
- GBF1 is recruited to the endogenous IRF-9 promoter, and interacts with C/EBP-beta, IL-1, and IL-6
- C/EBPbeta plays an essential constitutive role in the expression of COX-2, but may not be directly involved in its regulation in association with human labour.
- Identification of the CEBPB gene as a target of translational regulation in myeloid precursor cells transformed by the BCR/ABL oncogene.
- demonstrate a novel role for C/EBP-beta in interleukin-1beta-induced connective tissue disease and define a new nuclear target for the Extracellular Signal-Regulated MAP Kinases pathway in matrix metallopeptidase 1 gene activation
- Several DNA:transcription factor complexes were formed with the conserved regulatory regions identified in multi-species sequence alignment.
- Data imply that the comparison of C/EBP beta expression could be a prognostic marker for patients with glioma.
- identifies new function of C/EBPbeta in LDLR transcription as an oncostatin M-induced transactivator by itself in addition to its role as an EGR-1 co-activator
- ATRA triggers an increase in the translation of C/EBPbeta-LIP that antagonizes C/EBP transactivators by interacting with their binding sites in the albumin promoter
- constitutive expression of C/EBPbeta in ALK-positive anaplastic large cell lymphoma and its relationship to NPM-ALK
- C/EBP beta is an essential signaling component for inhibition of NF-kappa B-mediated transcription in TNF-tolerant monocytic cells.
- COX-2 is downregulated by PC-SPES via inhibition of NF-kappaB and C/EBPbeta in non-small cell lung cancer cells
- C/EBPbeta plays a key role in the coordination of TGFbeta cytostatic gene responses, and its malfunction may trigger evasion of these responses in breast cancer
- CCAAT/enhancer binding proteins alpha and beta have roles in growth and differentiation of hepatoblastomas
- Our data suggest that Tat-C/EBPbeta association is mediated through cdk9, and that phosphorylated C/EBPbeta may influence AIDS progression by increasing expression of HIV-1 genes.
- CCAAT/enhancer binding protein-beta is a mediator of PPAR delta-dependent 14-3-3 epsilon gene regulation in human endothelial cells.
- c-Rel((1-300)) binding to C/EBPbeta increases the affinity of C/EBPbeta for the C/EBP binding site at -53 on the CRP promoter
- diacylglycerol acyltransferase 2 expression is regulated by CAAT/enhancer-binding protein beta (C/EBPbeta) and C/EBPalpha during adipogenesis
- A switch between C/enhancer-binding protein (CEBP) zeta and CEBP beta operates at the CEBP site on the C-reactive protein (CRP)promoter to regulate CRP transcription.
- Important constitutive gene regulatory functions for C/EBPbeta in differentiated macrophages but not in human blood monocytes.
- data demonstrated that chronic inflammation appeared to play roles in induction of CCAAT/enhancer-binding protein beta expression in prostate epithelium which was associated with increased Cyclooxygenase-2 expression and androgen receptor downregulation
- C/EBPbeta is essential for the transcription of the 17beta-HSD8 gene in the liver
- KGF increased integrin alpha(5) expression by phosphorylating C/EBP-beta
- CCAAT/enhancer-binding protein beta may play a major role in interleukin-6-mediated inhibition of peroxisome proliferator activated receptor alpha gene expression.
- Results identify in Italians a suggestive linkage of early-onset type 2 diabetes (T2D) to chromosome 12q15, in the region of the CHOP gene, but no link to C/EBPbeta.
- IL-17 is a potent inducer of CRP expression via p38 MAPK and ERK1/2-dependent NF-kappaB and C/EBPbeta activation.
- C/EBP-beta participates in insulin-responsive expression of the F7 gene.
- The evidence is presented indicating that PDX-1 triggers hepatic dedifferentiation by repressing the key hepatic transcription factor CCAAT/enhancer-binding protein beta.
- PPARgamma agonists have diverging and overlapping mechanisms blocking transactivation of transcription factors NF-kappaB and C/EBPbeta.
- IL-17 induces MMP-1 in human cardiac fibroblasts directly via p38 MAPK- and ERK-dependent AP-1, NF-kappaB, and C/EBP-beta activation.
- C/EBPbeta was identified as the transcription factor regulating Tas1r3 promoter activity in HuCCT1 cells.
- The effect of cell density and inflammatory conditions on the expression, compartmentalization, activation, and the anti-proliferative function of the GR in primary human lung fibroblast cultures, was studied.
- C/EBPbeta, which binds to multiple cis-regulatory elements in promoter I.3/II, is a key factor in the transcriptional complex controlling aromatase expression in uterine leiomyoma cells.
- Pyk2 is induced and involved in monocyte differentiation and C/EBPbeta is a critical regulator of the Pyk2 expression.
- Lysophosphatidic acid-induced transactivation of epidermal growth factor receptor regulates cyclo-oxygenase-2 expression and prostaglandin E(2) release via C/EBPbeta in human bronchial epithelial cells.
- ERK-regulated site in Med1 protein is also essential for up-regulating interferon-induced transcription although not critical for binding to C/EBP-beta
- C/EBP beta overexpression significantly upregulated promoter activities of IL-8, COX-2, and anti-apoptotic Bfl-1 genes in prostate cancer cells.
- /EBPbeta as a direct substrate of G9a-mediated post-translational modification that alters the functional properties of C/EBPbeta during gene regulation.
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) showed decreased expression of HNF3 beta/FoxA2 and TTF-1 mRNA in papillary thyroid carcinoma cell lines, when compared with normal thyroid cells
- C/EBPbeta was found to have an important role in epithelial cell ICAM-1 regulation, while the adjacent NF-kappaB sequence binds the RelA/p65 and NF-kappaB1/p50 members of the NF-kappaB family to induce ICAM-1 expression in response to H. influenzae.
- NRAMP1 proximal region binds CCAAT enhancer binding proteins alpha or beta and is crucial for transcription
- These data indicate that NF-IL6 is a natural inhibitor of APOBEC3G that facilitates HIV-1 replication.
- the interplay between NF-kappaB and C/EBPbeta has an impact on the ability of C/EBPbeta to stimulate TGF-beta1 transcription
- Tumor necrosis factor-alpha upregulates 11beta-hydroxysteroid dehydrogenase type 1 expression by CCAAT/enhancer binding protein-beta in HepG2 cells.
- Regulates cytokine production in mature neutrophils; nuclear C/EBPbeta is rapidly phosphorylated upon cell stimulation, suggesting that it can activate cytokine promoters.