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Validated All-in-One™ qPCR Primer for IKZF1(NM_006060.3) Search again
Product ID:
HQP000397
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CVID13, Hs.54452, IK1, IKAROS, LYF1, LyF-1, PPP1R92, PRO0758, ZNFN1A1
Gene Description:
IKAROS family zinc finger 1
Target Gene Accession:
NM_006060.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- A novel Ikaros isoform "Ikx" is the predominant Ikaros protein present in normal cord blood and bone marrow cells, but not in leukemia cell lines.
- role in unconventional potentiation of gene expression
- Vasoactive intestinal peptide receptor-1 (VPAC-1) is a novel gene target of the hemolymphopoietic transcription factor Ikaros
- REVIEW:Role of ikaros gene expression and post-transcriptional mechanisms in leukemic processes.
- The expression of Ikaros in a panel of T-cell luekemia/lymphoma cell lines showed that irrespective of their developmental stages, the predominant isoforms epressed were Ik-1,2, and 3.
- In contrast to children, adult acute myeloid leukemia cells do not express nonfunctional Ikaros isoforms.
- Ikaros proteins function in myeloid as well as lymphoid differentiation, and specific Ikaros isoforms may play a role in regulating lineage commitment decisions.
- Ikaros has a role in progesterone activation of fatty acid amide hydrolase in human T lymphocytes
- Constitutional deletion of the ZNFN1A1 gene in a Greig cephalopolysyndactyly patient may have resulted in an increased risk of acute lymphoblastic leukemia.
- Wild-type Ikaros (Ik1) inhibits growth hormone mRNA and protein expression but stimulates prolactin mRNA and protein levels.
- Ik is involved in the regulation of STAT4 in human T cells.
- BCR-ABL1 induces aberrant splicing of IKAROS, which interferes with lineage identity and differentiation of pre-B lymphoblastic leukemia cells.
- Ikaros is involved in migration and invasion of extravillous trophoblasts in early placentation.
- Over-expression of a dominant interfering Ikaros isoform exclusively in B cells has profound effects on mature B cell function.
- Retardation gels showed binding activity for Ikaros, NFkappaB and AP4 transcription factors and mutations in their binding sites abolish Aiolos promoter activity.
- AChE-S mRNA induced selective bone marrow upregulation of Ikaros while suppressing FOG, another transcriptional partner of CtBP.
- the NuRD complex makes major contributions to the functions of both Ikaros and Helios and the activities of these proteins may be regulated in part by changes in phosphorylation
- showed Aiolos overexpression in primary lymphoma tissue
- findings suggest that genetic lesions resulting in the loss of Ikaros function are an important event in the development of BCR-ABL1 acute lymphoblastic leukaemia
- Ikaros regulates the cholesterol uptake metabolic pathway
- Genomic sequence and computational analyses of exon splice junction regions of IKZF1 in Ph+ ALL patients predicted several mutations that may alter alternative splicing.
- both aberrant splicing and genomic deletion leading to different non-DNA-binding Ikaros cDNA transcripts are common features of Philadelphia chromosome-positive acute lymphoblastic leukemia.
- Ikaros is active in erythropoiesis, especially fetal-to-adult globin switching. Dominant negative isoforms in precursor cells modify erythroid differentiation. Ikaros may control myeloid/erythroid commitment. Review.
- Genetic alteration of IKZF1 is associated with a very poor outcome in B-cell-progenitor ALL.
- Variable Ikaros isoform expression is associated with precursor-B acute lymphoblastic leukemia.