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Validated All-in-One™ qPCR Primer for HUWE1(NM_031407.6) Search again
Product ID:
HQP000096
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MRXST, MULE, URE-B1, UREB1
Gene Description:
HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1
Target Gene Accession:
NM_031407.6(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Ubiquitin ligase activity of ARF-BP1 (HUWE1) is inhibited by Arf.
- study modifies the current view of ARF-mediated p53 activation and reveals that ARF-BP1 is a critical mediator of both the p53-independent and p53-dependent tumor suppressor functions of ARF
- Mule (HUWE1) poly-ubiquitinates anti-apoptotic gene Mcl-1.
- Mule is both required and sufficient for the polyubiquitination of Mcl-1; Mule is a unique BH3-containing E3 ubiquitin ligase apical to Bcl-2 family proteins during DNA damage-induced apoptosis
- LASU1 is an E3 ligase that ubiquitinates Mcl-1 in vitro and is required for its proteasome-dependent degradation, the BH3 domain of LASU1 interactes with Mcl-1.
- HectH9-mediated ubiquitination of Myc is required for transactivation of multiple target genes.
- These findings demonstrate an important and conserved role for Huwe1 in regulating Cdc6 abundance after DNA damage.
- An increased gene dosage of HSD17B10, HUWE1, or both contribute to the etiology of X-Linked Mental Retardation and suggest that point mutations in HUWE1 are associated with this disease too.
- Huwe1 links destruction of N-Myc to the quiescent state that complements differentiation in the neural tissue
- Miz1 and Myc affect the activity of the Atr checkpoint through their effect on TopBP1 chromatin association and stability.