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Validated All-in-One™ qPCR Primer for CDH3(NM_001793.5) Search again
Product ID:
HQP000038
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CDHP, HJMD, PCAD
Gene Description:
cadherin 3
Target Gene Accession:
NM_001793.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq].
Gene References into function
- high levels of 80 kDa sP-cadherin in human milk.
- results point to the close relationship between the de novo expression of P-cadherin and the disruption of the cadherin/catenins complex with the squamoid phenotype of mucoepidermoid carcinoma of the thyroid (MECT)
- may play a central role in the development of endometriosis by mediating endometrial-peritoneal cell interactions in a homophilic manner
- Regulated expression of P-cadherin/beta-catenin complexes in ovarian tumor cells may represent a key step in disease progression
- Inappropriate PCAD expression in mouse mammary epithelium is compatible with normal mammary gland function.
- An absent P-cadherin expression could constitute a hallmark of aggressive biological behaviour in oral squamous cell carcinoma.
- In summary, we have ascertained a third gene associated with ectrodactyly and have demonstrated a hitherto unrecognised role of CDH3 in shaping the human hand
- A short truncated 50 kDa form of the N-terminal part of P-cadherin expresses in melanoma cell lines compared to melanocytes and keratinocytes.
- P-cadherin may have a role in progression of oral squamous cell carcinoma
- P-cadherin expression was strongly associated with tumor aggressiveness, being a good indicator of clinical outcome, and the aberrant expression of P-cadherin in breast cancer might be regulated by gene promoter hypomethylation.
- Novel heterozygous CDH3 mutations in hypotrichosis with juvenile macular dystrophy in two nonconsaguineous families.
- P-cadherin may be regarded as an additional immunohistochemical marker of basal-like breast carcinomas.
- P-cadherin is a newly defined transcriptional target gene of p63, with a crucial role in hair follicle morphogenesis as well as the apical ectodermal ridge during limb bud outgrowth in humans.
- Tunisian IBCs are characterized by frequent basal and ERBB2 phenotypes. Surprisingly, luminal inflammatory breast cancer also express the basal marker P-cadherin.
- This preliminary study provides evidence that the CK19/EpCAM2 and/or CK19/P-cadherin ratio(s) may be a simple and accurate prognostic indicator of clinical outcome in early-stage adenocarcinoma of the lung.
- P-cadherin expression in adenomas suggest that this molecule might have role in adenoma formation though not necessarily be involved in neoplastic progression.
- Results suggest that generation of truncated P-cadherin during the progression of oral squamous carcinoma attenuates tissue integrity, facilitates cellular separation, and leads to the acquisition of a more migratory phenotype.
- High levels of the soluble fragment of P-cadherin in Cancer Nipple aspirate fluid suggest its possible release via proteolytic processing
- Induced P-cadherin expression, especially in mucosal damage, leads to an increased rate of crypt fission, a common feature of clonal expansion in gastrointestinal dysplasia.
- Up-regulation of CDH3 is associated with pancreatic, gastric, and colorectal cancers.
- The identified mutation IVS10-1 G-->T is very likely to result in skipping of exon 11, leading either to synthesis of truncated protein or to an absence of CDH3 expression, possibly due to mRNA decay.