Product Information
GeneCopoeia provides high-quality enzyme reagents for the detection of novel coronavirus and other pathogens, including QuanTaq™ Taq-Ab DNA Polymerase, SW2050™ Reverse Transcriptase, and RNaseLock™ RNase Inhibitor. These products are ideally suited for both one-step and two-step probe-based qPCR assays.
RNaseLock™ RNase Inhibitor
- Source:RNase (Ribonuclease) Inhibitor is the porcine gene RNase inhibitor expressed in recombinant strain of E. coli.
- Performance: It effectively inhibits the activity of RNase A, RNase B, and RNase C in eukaryotic organisms, but does not inhibit the activity of RNase H, S1 nuclease, T7, SP6, and T3 RNA polymerases, M-MLV and AMV reverse transcriptases, or DNA polymerases.
- Advantage: It is capable of inhibiting RNase activity at temperatures up to 50°C.
- Application: It is widely used in experiments requiring the prevention of potential RNase contamination, such as cDNA synthesis, in vitro transcription, and translation.
Experimental example
Effectively inhibits the activity of RNase A
Figure 1.RNase Inhibitor of 40 U can effectively inhibit the activity of RNase A up to 80 ng.
In a 20 µl reaction system, add 15 ng of total RNA frommouseliver tissue and 40 U of RNase Inhibitor, along with different concentrations of RNase A. Incubate at 37℃ and observe the protective effect of the RNase Inhibitor on the RNA. 1:Total RNA from mouse liver tissue. 2:Total RNA and 15 ng RNase A. 3~7:Total RNA and 40 U RNase Inhibitor with different concentrations of RNase A
Broad thermal stability of RNase Inhibitor
Figure 2.RNase Inhibitor can protect RNA from degradation by RNase A at up to 50 ℃.
In a 20 µl reaction system, add 15 ng of total RNA frommouseliver tissue, along with 40 U of RNase Inhibitor and 15 ng of RNase A. Incubate at different temperatures to observe the protective effect of RNase Inhibitor on RNA. 1:Total RNA from mouse liver tissue. 2:Total RNA and 15 ng RNase A. 3~6:Total RNA and 15 ng RNase A with 40 U RNase Inhibitor at different temperatures
