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EndoFectin™ RNAi Transfection Reagent

EndoFectin™ RNAi transfection reagent EndoFectin™ RNAi transfection reagent

Price:	$307.00 $613.00
Catalog#:	EF021 EF022
XX:	1 mL 3 mL
Size:	1 mL 3 mL
Qty:
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EndoFectin™ RNAi

EndoFectin™ RNAi transfection reagent is a transfection reagent based on the principle of lipofectamine® transfection. It is specially designed to deliver siRNA and miRNA to mammalian cells. It can form a complex with nucleic acid and allow the complex to enter the cell. EndoFectin™ RNAi transfection reagent is widely applicable to common cell lines, such as HEK-293, HEK293T, Hela, NIH/3T3 and A549, etc. This reagent efficiently delivers nucleic acids into cells even in the presence of serum.

Advantages

Better transfection efficiency.
Low cytotoxicity.
For transfection of a variety of cell lines, easy to operate.
Does not require removal of serum or culture medium, or removal of medium after transfection.

Comparison of EndoFectin™ RNAi and Lipofectamine® 2000 in the siRNA transfection efficiency

Figure 1. EndoFectin™ RNAi and Lipofectamine®2000 transfection reagent were used to transfect 5 pmol and 10 pmol FAM-labeled β-actin-siRNA into HEK293T (A), Hela (B), and A549 (C). After 6 hours, the FAM green fluorescence intensity was observed through a fluorescence microscope.

Comparison of EndoFectin™ RNAi and Lipofectamine®3000 on eGFP gene in the knockdown effects

Figure 2. Use 1 μL EndoFectin™ RNAi and Lipofectamine®3000 transfection reagent respectively to transfect 5 pmol eGFP-siRNA into the Hela-eGFP stable cell line (cat#. SL040) in a 24-well plate. After 48 hours, changes in eGFP fluorescence intensity were observed through a fluorescence microscope.

Comparison of EndoFectin™ RNAi and Lipofectamine® RNAiMAX on eGFP gene in the knockdown effects

Figure 3. Use 1 μL EndoFectin™ RNAi and Lipofectamine® RNAiMAX transfection reagent respectively to transfect 5 pmol eGFP-siRNA into the HEK293T-eGFP stable cell line (cat#. SL039) in a 24-well plate. After 72 hours, changes in eGFP fluorescence intensity were observed through a fluorescence microscope.