All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by GeneCopoeia are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.

Protein Subcellular Localization & Validated Organelle Markers

The study of protein subcellular localization is necessary to elucidate protein function. To facilitate protein localization studies inside cells, organelles and tissues, GeneCopoeia offers multiple sets of expression-ready ORF cDNA constructs. These constructs consist of full-length mouse and human cDNA ORFs fused with various tags including eGFP, mCherry, SNAP-Tag™ and HaloTag®.

Fully-sequenced, expression-ready GeneCopoeia ORF cDNA tagged plasmids enable many studies including:

Subcellular protein localization in live and fixed cells
Monitoring of multiple proteins trafficking in one assay
Protein detection, tracking and characterization
Organelle morphology without antibodies
Co-localization (see figure 1)

Co-localization of two proteins in the same cell using OmicsLink™ ORF expression clones


1	2	3	4	5

Figure 1. TOMM20 (translocase of outer mitochondrial membrane 20 homolog) is located in the mitochondria. LEF1 (Lymphoid enhancer-binding factor 1) is located in the nucleus. Using standard cell and transfection techniques, cells were co-transfected with two plasmids; EX-G0283-M26 (encoding the TOMM20 gene fused with SNAP-Tag™ ) and EX-W0594-M50 (encoding the LEF1 gene fused with HaloTag®). Twenty-four hours after transfection, the cells were labeled with HaloTag® TMR ligand and SNAP Cell BG505 ligand according to the manufacturer’s instructions. The nuclei were stained with DAPI and the subcellular structures of the cells were imaged using fluorescent microscopy: (1) cells labeled with Halo-TMA ligand (red); (2) cells labeled with SNAP Cell BG505 ligand (green); (3) overlay of 1 and 2; (4) cells stained with DAPI; and (5) overlay of 1, 2 and 4.

Validated organelle markers and predicted subcellular localization genes

Click on each organelle name for gene list or Browse all validated organelle markers and predicted subcellular localization genes.

Human full-length ORF expression clones for studying protein localization

Vector	Promoter	Host Cell	Selection Marker	Tag	Protease Site
pReceiver-M97	EF1a	Mammalian	Neomycin	C-eGFP(monomeric)	N/A
pReceiver-M37	CAG	Mammalian	Neomycin	C-eGFP	N/A
pReceiver-M29	CMV	Mammalian	Neomycin	N-eGFP	N/A
pReceiver-M98	CMV	Mammalian	Neomycin	C-eGFP(monomeric)	N/A
pReceiver-M03	CMV	Mammalian	Neomycin	C-eGFP	N/A
pReceiver-M15	CMV	Mammalian	Neomycin	N-eYFP	N/A
pReceiver-M16	CMV	Mammalian	Neomycin	C-eYFP	N/A
pReceiver-M49	CMV	Mammalian	Neomycin	N-HaloTag	Tev protease
pReceiver-M50	CMV	Mammalian	Neomycin	C-HaloTag	Tev protease
pReceiver-M55	CMV	Mammalian	Neomycin	N-mCherry	N/A
pReceiver-M56	CMV	Mammalian	Neomycin	C-mCherry	N/A