Product Information
Poly(A) Polymerase (PAP) catalyzes the incorporation of adenine residues into the 3’-termini of various kinds of polyribonucleotides. PAP can utilize various kinds of single-stranded RNAs as substrates but not DNA. Double-stranded RNA and excessively short oligonucleotides are not recommended as substrates. ADP and dATP cannot be used as substrates. The incorporation of CTP and UTP is less than 5% of that of ATP, and the enzyme cannot incorporate GTP into the 3’-termini of polyribonucleotides.
Feature
The versatile regulatory capacity in RNA metabolism ensures precise gene expression by stabilizing mRNA, modulating translation, and dynamically responding to environmental changes.
Applications
- For 3′-terminilabeling of RNA;
- Poly(A) tailing of RNA for cloning or affinity purification;
- Enhancestranslation efficiency of RNA transfected into eukaryotic cells.
