All human and mice ORF clones and related Lentivirus, AAV particles and recombinant proteins provided by GeneCopoeia are fully sequenced using Sanger sequencing by capillary electrophoresis and Amino Acid Sequences are guaranteed to be matched with NCBI database.

GeneCopoeia miRNA Comprehensive Solutions — complete your understanding of miRNA with our wide range of functional analysis and detection tools: Transfection of target cells with miRNAs and generation of lentiviral particles

For studying the role of miRNA or trying to validate a putative target, GeneCopoeia has the right solutions. GeneCopoeia miRNA tools will help you design effective experiments to answer your questions.

What is the regulatory function of a specific miRNA? Do quantitative changes in its expression directly or indirectly lead to cancer or other diseases? If so, how much change?
How can I quickly detect and measure miRNA expression levels?
Which miRNA have an effect on my gene of interest?
Does a particular miRNA have the same influence on the same target gene in a different cell population?
Are there multiple targets for my miRNA? How can I validate a specific miRNA against a predicted target?

GeneCopoeia provides solutions for every step of miRNA functional analysis and detection

Transfection of target cells with miRNAs and generation of lentiviral particles

EndoFectin™ Transfection Reagents

A variety of optimized transfection reagents have been developed by GeneCopoeia resulting in efficient and easy transfection. Choose one to meet your needs:

For transfection of non-viral precursor and inhibitor miRNA plasmid constructs into most classical cell lines, EndoFectin PLUS is the efficient and cost effective choice.
For transfection of lentiviral vector-based precursor and inhibitor miRNA clones use EndoFectin Lenti reagent for both efficient transfection and high titers for particles.

Lenti-Pac™ Lentiviral Packaging kits

Lentiviral packaging kits have been optimized for use with GeneCopoeia lentiviral constructs to achieve high titers and robust expression levels.

Both HIV-and FIV-based lentiviral packaging kits are available and include EndoFectin Lenti and TiterBoost reagents for 10-fold higher titers.
The components of the kit together with miRNA plasmids can be co-transfected into GeneCopoeia 293Ta cells to produce lentiviral particles for further transduction into non-dividing or difficult-to-transfect target cells.

Lentifect™ 293Ta cells

For easy transfection and high level expression of viral proteins, GeneCopoeia offers 293Ta cells for co-transfecting miRNA constructs with packaging plasmids.

Questions? Contact us here or email directly at inquiry@genecopoeia.com.