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RNA Interference (RNAi) solution

GeneCopoeia RNAi solution include tools for your gene knockdown, gene regulation, and target validation studies. Tools for transient and stable transfection, and transduction (infection) into a broad range of cell types including the difficult-to-transfect, neuronal, primary as well as conventional dividing cells are available.

shRNA – gene knockdown Lentiviral and non-viral vector-based clones (plasmids) for genome-wide human, mouse and rat	Precursor miRNA – miRNA overexpression Lentiviral and non-viral vector-based clones (plasmids) for all known human, mouse and rat miRNA
miRNA inhibitors – miRNA knockdown Lentiviral and non-viral vector-based clones (plasmids); and synthetic oligonucleotides for all known human, mouse and rat miRNA	miRNA 3’ UTR targets – target gene validation Luciferase vector-based clones (plasmids) for genome-wide human, mouse and rat genes
miRNA qRT-PCR – miRNA detection and quantification Validated miRNA specific primers and SYBR® Green based detection kits

Figure 1: Vector-based shRNA or microRNA RNAi mechanism

RNA Interference (RNAi) solution

shRNA – gene knockdown

Precursor miRNA – miRNA overexpression

miRNA inhibitors – miRNA knockdown

miRNA 3’ UTR targets – target gene validation

miRNA qRT-PCR – miRNA detection and quantification