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Validated All-in-One™ qPCR Primer for CD86(NM_175862.4) Search again
Product ID:
HQP116639
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B7-2, B7.2, B70, CD28LG2, LAB72
Gene Description:
CD86 molecule
Target Gene Accession:
NM_175862.4(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms.
Gene References into function
- Structure in complex with CTLA-4; may represent a distinct signalling mechanism available to dimeric cell-surface receptors.
- Thus, this study is the first demonstration of a distinct signaling event induced by CD80 and CD86 molecules in B cell lymphoma.
- In AML, CD86 is a marker of monocytic/dendritic lineage
- The B7-CD28/CTLA-4 costimulatory pathway has a dominant role in regulating T-cell activation. Antagonists enable graft survival and suppress autoimmunity.
- a soluble form of CD86 encoded by an alternatively spliced transcript is present at elevated levels in blood in some leukaemia patients
- CD80 and CD86 molecules can substitute for each other in the initial activation of resting CD4(+) T cells and in the maintenance of their proliferative response
- expression, refolding, purification, characterization, and crystallization of the receptor-binding domain of human B7-2 is described; glycosylation is not important for proper folding of the receptor-binding domain of B7-2 nor for its binding to CTLA-4
- Leishmania major infection of macrophages cocultured with neutrophils results in a neutrophil-macrophage interaction via CD86 leading to IFN-gamma secretion and restriction of Leishmania growth.
- Impaired up-regulation of CD70 and CD86 in naive B cells from patients with CVID suggests an intrinsic signalling or expression defect at the level of naive B cells in type I CVID.
- polymorphisms have no association with type I diabetes among Finnish subjects
- Intense expression is an unfavorable prognostic indicator for differentiated thyroid carcinoma of children and adolescents
- After B7-1 and B7-2 induction, proximal tubular epithelial cells costimulate CD28 on T lymphocytes resulting in cytokine production.
- Data show that interaction between iC3b-opsonized apoptotic cells and immature dendritic cells down-regulated the expression of CD86 and up-regulated expression of CC chemokine receptor 7.
- The identical effects of B7-1 and B7-2 on regulation of human IL-2 gene transcription factors NF-kappa B and AP-1.
- c-MIR induced specific down-regulation of B7-2 surface expression through ubiquitination, rapid endocytosis, and lysosomal degradation
- B7-2 dimer observed in the B7-2/CTLA-4 complex displays a very hydrophilic dimer interface which provides a mechanism for preventing the formation of B7-1/B7-2 heterodimers
- A key mechanism in the pathogenesis of MS is the increased expression of CD86 and CD40L and the increased production of IL12 during disease progression.
- expression profiles and relative contribution in the porcine-human xenogeneic response
- results demonstrate that herpes simplex virus-2 infection effects the expression of B7 isoforms (B7-1 and B7-2) on monocytes in two ways with opposing outcomes
- Immature dendritic cells engulfed apoptotic and necrotic neutrophils, resulting in up-regulation of CD83 and class II major histocompatibility complex molecules, but down-regulation of CD40, CD80, and CD86
- results confirm that both the CD80 and CD86 molecules play an important role in the maintenance and amplification of the inflammatory process
- Decreased expression of CD86 antigen is associated with melanoma
- CD80 and CD86 activate T cells in IgA nephropathy, CD80/CD86 expressions correlated with renal function at the time of renal biopsy, and monocyte/macrophages and tubular epithelial cells act as antigen presenting cells
- CD86 and CD80 have opposite roles in the functioning of human regulatory T cells via CTLA-4 and CD28, an observation that has significant implications for manipulation of immune responses and tolerance in vivo.
- PIII also promoted a significant increase in the percentage of cells expressing CD86
- Engagement of B7-1/B7-2 molecules on human indoleamine 2,3-dioxygenase (IDO)-positive dendritic cells delivers a signal that is obligately required for the triggering of IDO activity during antigen presentation.
- CTLA-4 gene polymorphism may not play a role in the development of rheumatoid arthritis in Chinese.
- CD86 may play an important role in asthma pathogenesis.
- utilized as cellular attachment receptor by Adenovirus serotype 3 and thus may achieve both goals of cellular entry and evasion of the immune system
- insertion of CD80 and CD86 antigens into HIV-1 virions increases virus infectivity by facilitating the attachment and entry process due to interactions with their two natural ligands, CD28 and CTLA-4
- kK5 proteins of Kaposi's sarcoma associated herepsvirus does not affect class I expression but does downregulate human B7.2 molecules in a TAP/tapasin-independent manner
- CD86 was found to be concentrated within the cytoplasmic vesicles of macrophages and dendritic cells.
- Upregulation in the colonic mucosa of patients with ulcerative colitis.
- NF-kappaB signal plays a key role in LIGHT-mediated upregulation of CD86 expression.
- There was no relationship between the B7 gene polymorphisms studied and disease susceptibility or BAL fluid cell profiles in Japanese sarcoidosis patients.
- B7.1/B7.2 binding ultimately determines the formation of dimer-dependent CTLA-4 lattices that may be necessary for triggering B7-dependent T cell inactivation.
- Results indicate the expression of functional B7.2 molecule may facilitate progression of acute myeloid leukemia.
- CD86 AA genotype at +1057 position could be involved in liver transplant acceptance, given that its presence is related to a decrease of acute rejection frequency and to a graft survival increase.
- in human T4 cells in the absence of TCR ligation, CD28/CD86 interaction induced lipid raft polarization, activation of Vav1, increase in intracellular calcium, and nuclear translocation of NFKBp65, but not T cell proliferation or cytokine production
- While purified CD123+ plasmacytoid dendritic cells from adults up-regulated co-stimulatory molecules CD80 and CD86 with IL-3 alone those from neonates required the addition of CpG-DNA to reach adult levels.
- Expression of CD86 on keratinocytes from normal cervical epithelium but not on HPV-16 positive lesions could be a mechanism for evading host immune surveillance.
- The CD86 gene, and specifically the Ile179Val polymorphism, may be a novel aetiological factor in the development of asthma and related allergic disorders.
- Expression of CD86 and CD80 costimulatory molecules appears to be a marker of better clinical outcome and survival in patients with nasopharyngeal carcinoma.
- CD40, CD80 and CD86 are upregulated in cultured monocyte-derived dendritic cells of patients with coronary artery disease
- while CD86 does not stimulate an initial response as strongly as CD80, there is greater sustained activity that is seen even in the absence of continued costimulation
- T cells that had become non-responsive to anti-CD3 could be reactivated to proliferate when costimulated with 4-1BBL, either alone or combined with CD80/CD86.
- CD86 was constitutively expressed on circulating monocytes in healthy individuals and levels were decreased in septic subjects on Day 1
- Results describe a two-pronged mechanism by which Nef removes CD80 and CD86 from the cell surface.
- Dendritic cells obtained from Crohn's disease patients with mutations in the NOD2 gene display an activated phenotype characterized by high CD86 expression.
- possible functional relationship between the enhanced incidence of precursor plasmacytoid dendritic cells, their comparatively high relative expression of the coinhibitory molecule PD-L1
- Generation of a novel alternate transcript of the B7-2 costimulatory molecule (B7-2C) by the splicing of exon 4 appears to represent a mechanism for the fine tuning of full-length protein B7-2A-mediated costimulatory signals.
- CD86 was differentially expressed in both macrophages and foam cells from subjects with atherosclerosis.
- The expression levels of B7.2 were low on day 1 in S7, S10 and H37Ra Mycobacterium tuberulosis infected monocyte derived macrophages
- Monocytes of chronic graft-versus-host disease patients had greater CD86 mean fluorescence intensity in marrow.
- A novel role is found for CD86 expression on the microvasculature, whereby ligation of CTLA-4 on CD4-positive T cells by CD86 on islet endothelial cells is key to the adhesion of recently activated T cells.