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Validated All-in-One™ qPCR Primer for CD40LG(NM_000074.2) Search again
Product ID:
HQP022962
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Description:
CD40 ligand
Target Gene Accession:
NM_000074.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq].
Gene References into function
- Review. In some cases of CLL the malignant cells express both CD40 and CD154. Implications for autoimmunity and therapy are discussed.
- The capacity of natural killer cells to induce B cell activation is regulated by the interaction of CD40 with its ligand CD154.
- the CD40L gene 3'-flanking region acts as a T cell-specific classical transcriptional enhancer by a NF-kappaB p50-dependent mechanism.
- CD40/CD40L interactions are important for the activation of macrophages as effector cells that mediate inflammation and tissue damage in T cell-mediated inflammatory processes.
- CD40 ligand contacts between fibroblasts and cells secreting IL-4 may promote the profibrotic effects of IL-4 by affecting signal transduction and reducing the anti-fibrotic effects of IFN-gamma.
- CD40L-deficient T cells in carriers of X-linked hyper-IgM syndrome are minimally impaired in comparison with CD40L-expressing T cells in several parameters of T cell priming.
- sCD40L levels in blood, quantities released from platelets ex vivo, and quantities released from SFFLRN stimulated platelets ex vivo were compared for preeclamptic and normal pregnancies.
- CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes.
- CD40 and CD40L are important in autoimmunity and other immune processes. At least 5 signal transduction pathways are involved.
- review of the role of CD154 and the type-1 cytokine response
- CD40L appears to be an alphaIIbbeta3 integrin ligand, a platelet agonist, and necessary for stability of arterial thrombi.
- Latent sensitivity to Fas-mediated apoptosis after CD40 ligation may explain activity of CD154 gene therapy in chronic lymphocytic leukemia
- Role of surface IgM and IgD on survival/apoptosis of B-cell chronic lymphocytic leukemia cells.
- Association of CD40 ligand expression on HTLV-I-infected T cells and maturation of dendritic cells.
- dinucleotide microsatellite in multiple sclerosis
- Elevated soluble CD40 ligand is related to the endothelial adhesion molecules in patients with acute coronary syndrome.
- Burkitt lymphoma cell population depletion activates autonomous CD154-dependent survival.
- role in inducing tissue factor expression in endothelial cells
- Levels of soluble CD40 ligand (CD154) in serum are increased in human immunodeficiency virus type 1-infected patients and correlate with CD4(+) T-cell counts
- Clustering of CD40 ligand is required to form a functional contact with CD40
- cd154 (cd40 ligand)is expressed during treatment with calcineurin inhibitors after organ transplantation.
- Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent
- CD40 ligand (CD154) does not contribute to lymphocyte-mediated inhibition of virulent Mycobacterium tuberculosis within human monocytes
- association of a common variant in CD40L with a malaria resistance phenotype
- effect of platelet-derived CD40L on the tissue factor activity of human CD40-positive melanoma cells and monocytes
- In human lung myofibroblasts,CD40 ligand is induced by lipopolysaccharide, thrombin and TNF-alpha.
- CD154 expression in renal cell carcinoma
- Treatment of human gingival fibroblasts with human leukocyte elastase down-regulated CD40 binding to CD40 ligand. HLE treatment of HGF decreases IL-8 and macrophage chemoattractant protein-1 production by HGF when stimulated by CD40L.
- Impact of both donor and recipient strains on cardiac allograft survival after blockade of the cd40 costimulatory pathway
- upregulation of CD40L on platelets triggers CD40L-dependent matrix degradation by vascular endothelial cells
- CD40L induces proliferation, self-renewal, rescue from apoptosis, and production of cytokines by CD40-expressing AML blasts.
- CD40L has a role in activating antigen-presenting cells (APCs) and thereby initiating the human immune response in HIV infection
- findings suggests that there are two groups of immune thrombocytopenic purpura (ITP) patients, one with elevated and one with normal of sCD40L; pathogenesis of ITP may in some patients include alterations of the CD40/CD40L pathway
- CD154 function is determined by binding to PTB and PTB-T and is involved in autoimmune disease
- CD40L expression on CD4+ T-cells is significantly higher in patients with Kawasaki disease (KD) than in the febrile control group, which implies that CD40L over-expression might play a role in the immunopathogenesis of KD.
- CD40L can induce maturation of monocyte-derived dendritic cells and elicit sustained antiviral cytotoxic T lymphocyte responses, either alone or cooperatively with TNF-alpha or RANKL.
- Proapoptotic genes BAX and CD40L are predictors of survival in transitional cell carcinoma of the bladder.
- Serum levels of sCD40L significantly higher in MCTD than in healthy individuals. May play role in pathogenesis of MCTD.
- A key mechanism in the pathogenesis of MS is the increased expression of CD86 and CD40L and the increased production of IL12 during disease progression.
- high shear stress induces CD40L translocation to the platelet surface, which is mediated by the von Willebrand factor (VWF)-GP Ibalpha interaction and enhanced in the presence of a low concentration of epinephrine
- Data suggest that CD40L-expressing cells may act in combination with thymic stromal lymphopoietin to amplify and sustain pro-allergic responses.
- CD40L expression by BCG-activated CD4(+) T cells is regulated via the PKC pathway and by NF-kappaB DNA binding activity
- Individuals with type 1 or 2 diabetes have a proinflammatory state as indicated by elevated levels of plasma sCD40L. Troglitazone treatment of type 2 diabetic patients diminishes sCD40L levels
- Patients with acute cerebral ischemia show upregulation of the CD40 system, which might contribute to the known proinflammatory, proatherogenic, and prothrombotic milieu found in these patients
- Local infiltration of skin with cells expressing human CD40L on their cell surface mediates the induction of vascular endothelial growth factor and fibroblast growth factor in vivo and results in a marked angiogenesis reaction.
- Activated platelets trigger dendritic cell maturation independent of cyclooxygenase-derived arachidonic acid metabolites by mechanisms involving CD40LO, which is also involved in monocyte chemotactic mediator release from platelets and dendritic cells.
- Results indicate that patients with active lupus nephritis exhibit B cell abnormalities that are consistent with intensive germinal center activity, are driven via CD154-CD40 interactions, and may be involved in the production of autoantibodies.
- Increased sCD40L is associated with late restenosis after PTCA. This may provide a link between restenosis and aspirin-insensitive platelet activation. These results provide a rationale for studies with new antiplatelet treatments in PTCA patients
- CD154 regulates TNF-alpha-dependent antiprotozoal activity in monocyte-derived macrophages when interferon-gamma signaling is deficient.
- Patients with diabetes show increased coexpression of CD40 system, especially CD40L, which may create a proinflammatory and prothrombotic milieu for aggravating the development of atherosclerosis.
- Human lung fibroblasts express CD40L in vitro and in situ; the level of CD40L expression by lung fibroblasts is increased in fibrotic compared with normal tissue.
- beta2-AR agonists strongly inhibited the expression of ICAM-1 and CD40.
- CD40 ligand has a role in platelet activation in lung cancer
- CD40L-positive T cells induce platelet activation through a contact-mediated, CD40-dependent pathway resulting in RANTES release. Soluble CD40L induces the same events via p38, but not extracellular signal-regulated kinase, phosphorylation.
- Thrombin-induced platelet P-selectin expression was enhanced, and soluble P-selectin and sCD40L concentrations were increased in patients with microangiopathy.
- By way of CD154-induced CD154 expression, human endothelial cells thus seem capable of influencing the progression of proinflammatory reactions, including atherogenesis through activation of extravasating monocytes.
- The CD4+ T lymphocyte-dependent antibody response to pneumococcal capsular polysaccharides requires CD40-CD40 ligand interaction.
- CD28i, by functioning as a signaling adaptor, transduces CD40L signaling as well as CD28 signaling in human T cells.
- reduction in anti-IgM-induced growth inhibition due to altered N-glycosylation may enhance CD40-CD40L-mediated cell survival through TRAF2 which interacts with both IgM and CD40 in HBL-2 cells
- Serum levels in type 1 diabetes are not different from normal values.
- In patients with unstable coronary artery disease, elevation of serum soluble CD40L levels indicated an independent increased risk of major adverse cardiovascular events
- In an experimental murine model, transgenic mice expressing CD40 ligand ectopically on B cells spontaneously develop severe transmural intestinal inflammation in both colon and ileum at 8-15 wk of age.
- REVIEW: CD40-CD154 interaction can upregulate costimulatory molecules, activate antigen-presenting cells, influence T-cell priming and T-cell-mediated effector functions as well as participate in the pathogenic processing of chronic inflammatory diseases
- platelet-associated CD154 expression is increased in immune thrombocytopenic purpura and is able to drive the activation of autoreactive B lymphocytes in this disease
- Platelet CD40L expression occurs via arachidonic acid-mediated gp91phox activation.
- effect of platelet collaagen receptor glycoprotein IV (GPVI}-mediated release of CD40L on activation of endothelial cells.
- CD40L may have a role in the pathogenesis of PAH, possibly operating through an interaction between platelets and endothelial cells involving chemokine-related mechanisms.
- impaired IL-12 and tumor necrosis factor-alpha production in Sezary syndrome (SzS) is associated with defective CD4+ T lymphocyte CD40L induction and indicate that CD40L may have therapeutic potential in SzS
- CD40L-positive platelets induce CD40L-endothelial expression by human intestinal microvascular endothelial cells. CD40L-platelet-dependent CD40L-endothelial upregulation may be a new pathway in the amplification of endothelium-dependent inflammation.
- autocrine VEGF as an important mediator of the antiapoptotic effect of CD40 ligation, and thus provide new insights into CLL-cell rescue by CD154 in lymphoreticular tissues.
- TSLP is a major regulatory cytokine for CD40 ligand-induced IL-12 production by DCs, and TSLP-activated DCs could promote the persistence of Th2 inflammation even in the presence of IL-12-inducing signals
- the majority of thyroid cancers express CD-40 and CD-40 ligand
- In CD4+ T cells infected with wild-type virus, Nef is the viral factor that interferes with the immune mechanisms that regulate expression of CD154.
- role of recipient CD40 and CD154 in the rejection process of concordant and discordant islet xenotransplantation
- Upregulation of soluble CD40L as a consequence of persistent hyperglycaemia in diabetic patients may contribute to accelerated atherosclerosis development in diabetes.
- C3 secretion induced by CD40L may represent a mechanism of amplification of tubulointerstitial damage associated with lymphocyte infiltration.
- CD40L-transduced chronic lymphocytic leukemia cells are able to induce an antigen-specific T-cell response
- Ileal pouch mucosa leukocytes presented a significantly higher expression of CD40 and CD40L after proctocolectomy for ulcerative colitis and this alteration correlated with pouchitis
- Patients with unstable angina show an enhanced coagulation activation and an upregulation of CD40L on platelets.
- CD40L might contribute to the initiation and progression of atherosclerosis by increasing activity of prostacyclin synthase.
- Platelets from patients with systemic lupus erythematosus can activate mesangial cells through CD40/CD154 interactions
- Data show that reciprocal translocation caused disruption of CD40LG, resulting in defective CD40L expression with a skewed X-inactivation pattern in T cells leading to the HIGM1 phenotype.
- changes in circulating tissue factor procoagulant activity (PCA) and other procoagulation proteins in healthy volunteers exposed to 24 h of selective hyperinsulinemia, selective hyperglycemia, or combined hyperinsulinemia and hyperglycemia
- Overexpression of Egr-1, but not Egr-3, is capable of augmenting transcription of an intact CD154 promoter.
- The expression of CD69 and CD154 molecules depend partially on the prolactine.
- Activates the Jun amino-terminal kinase pathway through transient phosphorylation of Burkitt lymphoma cells in vitro.
- higher inflammatory status of coronary lesions as well as involvement of the CD40-CD154 signaling cascade in chronic renal failure patients, especially in cases of calcified atherosclerotic lesions
- Allograft rejection can be instigated by activated platelets through CD154.
- CD40L expressed on adjacent non tumoral cells induces multidrug resistance to cytotoxic agents and ceramides in both breast carcinoma and non Hodgkin's lymphoma cell lines, albeit through a caspase independent and dependent pathway respectively
- Patients with the metabolic syndrome have enhanced values of plasma CD40L.
- CD154 can sensitize leukemia cells to apoptosis via the c-Abl-dependent activation of p73 and mitigate the resistance of p53-deficient CLL cells to anticancer drug therapy.
- In both controls and patients with stable intermittent claudication support evidence for the role of CD40L in atherogenesis and acute peripheral arterial disease.
- Aspirin resistance is connected with higher sCD40L level at rest and exercise provoked aspirin resistance is connected with the sCD40L concentration increase.
- prolonged kidney graft survival when tranfected into rats.
- Additive effects between soluble CD40L and Rickettsia africae may contribute to endothelial inflammation and hypercoagulation in patients with African tick bite fever.
- Islet beta-cells responded to CD40L interaction by secreting IL-6, IL-8, MCP-1, and MIP-1beta. CD40-CD40L interaction activates extracellular signal-regulated kinase 1/2 and nuclear factor-kappaB pathways in insulinoma NIT-1 cells.
- Membrane and soluble forms are differentially regulated depending upon the activation stimulus, regulated cleavage may likely contribute to disease mechanisms.
- enhanced CD154 expression CD4+ and CD8+ lymphocytes of HIV-exposed noninfected infants, who have been exposed to antiretroviral drugs in fetal and early life.
- Significant differences were found between systemic lupus erythematosus patients and controls (p=0.02).
- A novel missense mutation, Leu225Ser, was recognized in connection with X-linked hyper-IgM syndrome.
- Compared with healthy controls, CD4(+) T cells from HIV-1(+) patients had impaired induction of CD154 when T cell activation was mediated by CD40(+) APCs. In contrast, T cell activation in the absence of these cells resulted in normal CD154 expression
- the amount of NF-ATc2 bound to the promoters of CD154 (CD40L) and IL-2 genes in SLE; although high NF-ATc2 levels translated into higher CD154 transcription in SLE, IL-2 transcription was decreased
- substitution of a D-prolyl residue for the glycyl within the Lys-Gly-Tyr-Tyr CD40-binding motif leads to a complete loss of cooperativity in the interaction of the CD40L mimetic with its cognate receptor.
- These results show that CD40L can influence RANKL signaling through T cell priming, and thus they demonstrate a regulatory role for CD40L in bone mineralization that is absent in patients with X-linked hyper-IgM syndrome.
- observations identify interaction of CD40L and Mac-1 as an alternative pathway for CD40L-mediated inflammation; this novel mechanism expands understanding of inflammatory signaling during atherogenesis
- Incorporation of host-encoded CD40L in HIV-1 is likely to play a role in the B-cell abnormalities that are seen in HIV-infected individuals.
- platelet-derived CD154 may be a key 'cytokine' responsible for adverse reactions associated with platelet transfusions
- The whole blood gene expression quantities of costimulatory molecules CD154 and ICOS reasonably robustly differentiated rejection patients from control patients.
- CD40 ligand has a role in peripheral arterial disease
- CD40-40L signaling has a role in vascular inflammation
- These results suggest that engagement of beta1 integrins on systemic lupus erythematosus T cells could induce FAK-mediated signaling and subsequent CD40L expression and proliferation.
- Decreased frequency of MHC class II-restricted CD4(+) regulatory T cells in CD40L-deficient patients suggests that these T cells may mediate B cell tolerance
- Vascular smooth muscle cells-like endothelial cells appear to contribute to the maintenance of an inflammatory response in the atherosclerotic vessel wall upon CD40-CD154 co-stimulation.
- This study shows that xenogeneic interaction between hCD40L and pCD40 can activate porcine endothelial cells through NF-kappaB signaling.
- Dyslipidemic patients show increased plasma and decreased platelet-membrane CD40L expression compared with dyslipidemic patients with low insulin resistance.
- A possible role of CD40-CD40L interactions between monocytes and T4 helper cells in the pathogenesis of bone marrow failure in myelodysplastic syndromes was shown.
- CD154 microsatellite contributes to the regulation of mRNA and protein expression.
- the magnitude of T-cell expression, such as increased expression of HLA-DR and CD40L, contributes to myocardial dysfunction in dilated cardiomyopathy
- CD40 ligand expression on stimulated T-helper lymphocytes of Common variable immunodeficiency patients is similar to normal controls
- Soluble CD40L level is a predictor of vascular events in patients with nonvalvular atrial fibrillation.
- Lack of association between soluble CD40L and risk in a large cohort of patients with acute coronary syndrome in OPUS TIMI-16.
- Demethylation of CD40LG and perhaps other genes on the inactive X may contribute to the striking female predilection of this disease
- Of 9 CVID patients...we identified... 1 patient with decreased CD40L expression who had a mutated (Thr254Met) extracellular domain of CD40L (782T>C in exon 5).
- abnormal indices of angiogenesis in coronary artery disease may be associated with increased CD40-CD40L interactions in patients with pathologic collateralisation
- hu5C8 MoAb has a strong mitogenic activity when immobilized, likely due to higher crosslinking capacity as compared to the soluble antibody
- Data show that MCP-1 has a synergistic effect on COX-2 and CCR2 protein expression in CD40L-stimulated HUVECs and stimulates VEGF production in these cells.
- These findings establish an immune-regulatory role for activin-A in dendritic cells, highlighting the potential of antagonizing activin-A signaling in vivo to enhance vaccine immunogenicity.
- platelet-associated CD154 can be biochemically modulated.
- Plasma soluble CD40 ligand and stimulated MIP-1alpha production were both reduced (p < or = 0.05) by systemic beta-adrenergic stimulation.
- the CD40/CD154 interaction has a role in the pathogenesis of autoimmune process leading to inflammatory infiltration in Graves' ophthalmopathy
- Neither cell-surface nor soluble CD154 levels are associated with coronary artery disease in systemic lupus erythematosus
- In heart failure patients, platelet CD40L is upregulated by TNFalpha via a cyclooxygenase-1-independent, arachidonic acid-mediated oxidative stress mechanism.
- Patients with chronic heart failure (CHF) showed increased expression of CD40L system, which may create a pathogenic role in the development and progression of CHF.
- The enhanced sCD40L and cellular CD40L expression in the metabolic syndrome suggests that CD40L is of pathophysiological relevance.
- Elevated sCD40L promotes platelet-leukocyte activation and recruitment and neointima formation after arterial injury.
- MCP-1 and CD40L had a synergistic effect on COX-2 expression and subsequent VEGF production in gastric cancer.
- no support for specific CD40L SNPs in the susceptibility to Kawasaki disease in Taiwanese children
- CD40 expression in tumors is associated with a poor prognosis and that the juxtacrine interaction of CD40-CD154 among cancer cells facilitates the development of malignant potential in nonsmall cell lung cancer.
- reversion of Fas resistance is mediated through CD40/CD40L ligation rather than IFN-gamma stimulation by inhibiting synthesis of c-FLIP
- Theses results suggest that CD40 is present in colon cancer, and recombinant soluble human CD40 ligand may be of clinical use to inhibit human colon cancer growth.
- Atrial fibrillation patients had significantly higher sCD40L levels compared to healthy control subjects with no difference in platelet surface CD40L and pCD40L levels.
- Results describe the clinical implications of serum-soluble CD154 (sCD154) levels and the expression of CD40 on monocytes in patients with fulminant hepatic failure.
- CD40 ligand has a role in inducing endothelial dysfunction in human and porcine coronary artery endothelial cells
- CD40-CD40 ligand interaction on Burkitt lymphoma cells was attributable to the suppression of NF-kappaB binding activity
- maturation of human DC with OK432 induces direct tumor cell killing by DC by interaction of tumor cell CD40 with dendritic cell CD40L
- study revealed statistically significant higher baseline concentrations of sCD40L--but not sP-selectin--in the group of asthmatics with exercise-induced bronchoconstriction than in those without
- Persistently increased sCD40L levels from the onset of diabetes might help to identify those normotensive and normoalbuminuric young patients at increased risk of developing incipient nephropathy later in life.
- In hypercholesterolemic Type 2 diabetic patients, sCD40L, a factor playing a pivotal role in the pathogenesis of atherosclerosis and associated with more rupture-prone lesions, is reduced by short-term treatment with rosuvastatin.
- Obstructive sleep apnea is associated with upregulation of circulating sCD40L levels and platelet-monocyte aggregation.
- TRAF2 overexpression in differentiated cells decreases the c-Jun N-terminal kinase-mediated synthesis and the secretion of proinflammatory cytokines, such as interleukin-8 and monocyte chemoattractant protein 1 (MCP-1) in response to CD40 ligand
- study demonstrated that Abeta(1-40), levels of sCD40 and sCD40L are increased in Alzheimer's disease and declining MMSE scores correlated with increasing sCD40L, which in turn, correlated positively with Abeta(1-42)
- sCD40 natural isoforms encompassing either three or four CRDs might exert different antagonistic effects from known CD154 antibodies by recognition of only membranous CD154.
- abnormally increased in patients with obstructive sleep apnea syndrome
- Elevated circulating levels of soluble CD-40 ligand in haemodialysis patients with symptomatic coronary heart disease.
- The present study investigated the molecular defects underlying disease in 4 patients with Type 1, X-linked Hyper-IgM syndrome 4 distinct CD40L mutations, 2 of them which have not been previously described.