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Validated All-in-One™ qPCR Primer for CD80(NM_005191.3) Search again
Product ID:
HQP022722
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
Gene Description:
CD80 molecule
Target Gene Accession:
NM_005191.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.[supplied by OMIM].
Gene References into function
- Thus, this study is the first demonstration of a distinct signaling event induced by CD80 and CD86 molecules in B cell lymphoma.
- specific small molecule inhibitors of human B7.1 were identified and characterized. These compounds inhibit the binding of B7.1 to both CD28 and CTLA4.
- The B7-CD28/CTLA-4 costimulatory pathway has a dominant role in regulating T-cell activation. Antagonists enable graft survival and suppress autoimmunity.
- Blockade of B7/CD28 costimulation in mixed lymphocyte reaction cultures results in the generation of alternatively activated macrophages, which suppress T-cell responses,and perhaps play a critical role in the induction of transplantation tolerance.
- colocalization of ICAM-1 and B7.1 molecules was demonstrated in Hashomoto's thyroiditis whereas no B7.1 expression was observed in Graves' disease
- CD80 and CD86 molecules can substitute for each other in the initial activation of resting CD4(+) T cells and in the maintenance of their proliferative response
- Leishmania major infection of macrophages cocultured with neutrophils results in a neutrophil-macrophage interaction via CD80 leading to IFN-gamma secretion and restriction of Leishmania growth.
- expansion of CD5- B cells in multiple sclerosis correlates with CD80 (B7-1) expression
- CD40 and CD80 molecules were observed to play a specific role in the induction of cytotoxic function but not in IFN-gamma production of IL-2-activated NK effectors.
- with ctla4 pathway, is essential for generating regulatory cells after intratracheal delivery of alloantigen in mice
- CD80 and CD86 differ in their interactions with CTLA-4 and that CD80 appears to be the preferential inhibitory ligand for CTLA-4 working via a population of CD4(+) CD25(+) CTLA-4(+) regulatory T cells.
- After B7-1 and B7-2 induction, proximal tubular epithelial cells costimulate CD28 on T lymphocytes resulting in cytokine production.
- data suggest that CD80 acquisition by human T cells might play a role in the immunoregulation of T cell responses
- The identical effects of B7-1 and B7-2 on regulation of human IL-2 gene transcription factors NF-kappa B and AP-1.
- On the biological relevance of MHC class II and B7 expression by tumour cells in melanoma metastases.
- combined use of a vector driving the expression of OX40L with three other costimulatory molecules (B7-1, ICAM-1, and LFA-3) both enhances initial activation and then further potentiates sustained activation of nai;ve and effector T cells.
- ICOS-B7H costimulatory pathway may be involved in the negative regulation of cell-mediated immune responses.
- expression profiles and relative contribution in the porcine-human xenogeneic response
- effect of PGE2 on the expression of ICAM-1 and B7 in the human mixed leukocyte reaction (MLR) in the presence or absence of IL-18
- results show that BCL6 prevents CD40-induced expression of CD80 by binding its promoter region in vivo and suppressing its transcriptional activation by NF-kappaB
- results demonstrate that herpes simplex virus-2 infection effects the expression of B7 isoforms (B7-1 and B7-2) on monocytes in two ways with opposing outcomes
- Immature dendritic cells engulfed apoptotic and necrotic neutrophils, resulting in up-regulation of CD83 and class II major histocompatibility complex molecules, but down-regulation of CD40, CD80, and CD86
- The CD80 immunoglobulin constant-like (C)-domain has a negative/regulatory effect on the immune response to a plasmid DNA vaccine; loss of the CD80 C-domain prevents modulation of the immune activation signal.
- results confirm that both the CD80 and CD86 molecules play an important role in the maintenance and amplification of the inflammatory process
- CD80 and CD86 activate T cells in IgA nephropathy, CD80/CD86 expressions correlated with renal function at the time of renal biopsy, and monocyte/macrophages and tubular epithelial cells act as antigen presenting cells
- CD80 and CD86 have opposite roles in the functioning of human regulatory T cells via CD28 and CTLA-4, an observation that has significant implications for manipulation of immune responses and tolerance in vivo.
- Engagement of B7-1/B7-2 molecules on human indoleamine 2,3-dioxygenase (IDO)-positive dendritic cells delivers a signal that is obligately required for the triggering of IDO activity during antigen presentation.
- utilized as cellular attachment receptor by Adenovirus serotype 3 and thus may achieve both goals of cellular entry and evasion of the immune system
- insertion of CD80 and CD86 antigens into HIV-1 virions increases virus infectivity by facilitating the attachment and entry process due to interactions with their two natural ligands, CD28 and CTLA-4
- B7-1 gene transduction might be effective against peritoneal metastases of gastric cancer.
- CD80 has a role in activating cytotoxic T lymphocytes in hepatocellular carcinoma cells
- CD80 driven by NF-kappaB is regulated by the enzymatic actions of caspases, which allows monocytes to participate in massive T-cell activation
- data demonstrated a correlation between HIV infection and impairment of CD80 by circulating monocytes
- There is a potential role of B7 co-stimulatory molecules in facilitating hepatocellular carcinoma escape from immune surveillance without the involvement of IL-10.
- CD80 binding polyproline helical peptide has a role inhibiting T cell activation
- Upregulation in the colonic mucosa of patients with ulcerative colitis.
- There was no relationship between the B7 gene polymorphisms studied and disease susceptibility or BAL fluid cell profiles in Japanese sarcoidosis patients.
- B7.1/B7.2 binding ultimately determines the formation of dimer-dependent CTLA-4 lattices that may be necessary for triggering B7-dependent T cell inactivation.
- LPS-induced B7.1 transcription in human monocytic cells may be regulated by JNK-mediated activation of the IRF-7 transcription factor.
- Th2 cytokine predominant in tumor microenvironment might be related to the expression of B(7)H(1),B(7)H(2) co-signal molecules in tumor cells and TIL
- CD80 transfection can lower malignant phenotype of hepatocarcinoma cells and has a down-regulatory effect to activated T cells in vitro.
- CD80-CD24 molecule spontaneously incorporated onto cell membrane through its glycolipid anchor suggesting that this hybrid costimulatory molecule can be used in protein transfer to develop effective cancer vaccines
- While purified CD123+ plasmacytoid dendritic cells from adults up-regulated co-stimulatory molecules CD80 and CD86 with IL-3 alone those from neonates required the addition of CpG-DNA to reach adult levels.
- CD80 expression during early oral squamous cell carcinoma formation may contribute to the escape of tumors from immune elimination
- Transduction of HMCLs with B7-1 retroviruses induced a high expression of B7-1 molecule and a strong T-cell activation ability.
- These results define elevated populations of memory T-lymphocytes expressing B7 molecules in rheumatoid arthritis (RA) synovial fluid that either stimulate T cells through ICOS, or down-regulate RA synovial tissue T-lymphocytes through B7H1 and B7H2.
- Expression of CD80 and CD86 costimulatory molecules appears to be a marker of better clinical outcome and survival in patients with nasopharyngeal carcinoma.
- CD40, CD80 and CD86 are upregulated in cultured monocyte-derived dendritic cells of patients with coronary artery disease
- sCD80 in human serum adds a new member to the family of soluble receptors, implying a network of soluble costimulatory factors with functional relevance
- T cells that had become non-responsive to anti-CD3 could be reactivated to proliferate when costimulated with 4-1BBL, either alone or combined with CD80/CD86.
- CD80 expression was up-regulated on the circulating monocytes in septic subjects on Day 1
- Results describe a two-pronged mechanism by which Nef removes CD80 and CD86 from the cell surface.
- CD80 and 4-1BBL induce auto- and transcostimulation in tumor cells
- this review focuses on inhibitory B7 family memberys in human ovarian carcinoma
- During infection when the expression of B7.1 is downregulated, other co-stimulatory molecules may take over its crucial role to confer protective immune response against M. tuberculosis
- Interaction of human PD-L1 and B7-1.
- In the T cell-dendritic cell synapse, transgenic murine CD80 clusters are colocalized with CD28 and protein kinase C theta, a characteristic of the central supramolecular activation cluster.
- CD80 antigen may prevent monocyte activation and may be required for successful allograft transplantation.
- urinary soluble CD80 is elevated in idiopathic minimal-change disease, which could be relevant to both diagnosis and pathogenesis