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Validated All-in-One™ qPCR Primer for CD276(NM_001024736.1) Search again
Product ID:
HQP019700
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
4Ig-B7-H3, B7-H3, B7H3, B7RP-2
Gene Description:
CD276 molecule
Target Gene Accession:
NM_001024736.1(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM].
Gene References into function
- A novel structural variant of B7-H3 (named B7-H3b) with four Ig-like domains, is the major isoform expressed in several tissues and results from gene duplication and differential splicing.
- B7-H3 enhances T lymphocyte proliferation and IL-10 secretion when expressed in E. coli in vitro
- data suggest that 4Ig-B7-H3 molecules expressed at the neuroblastoma tumor cell surface can exert a protective role from natural killer-mediated lysis by interacting with a still undefined inhibitory receptor expressed on natural killer cells
- Single nucleotide polymorphisms in the B7H3 gene are not associated with autoimmune myasthenia gravis.
- B7-H3 may be important for the interactions between fibroblast-like synoviocytes and T cells in rheumatoid arthritis and other diseases; the outcome of such interactions depends on the activation state of the T cell.
- analysis of an interaction between B7-H3 and TLT-2 that preferentially enhances CD8(+) T cell activation
- B7-H3 is highly expressed in urothelial cell carcinoma across tumor stages, whereas B7-H1 and PD-1 expression are associated with advanced disease
- Overexpression of B7H3 is associated with tumor cell migration and invasion.
- Both tumor cell and tumor vasculature B7-H3 expression convey important information to predict clear cell renal cell carcinoma outcomes
- Progenitor cells from the anterior pituitary gland containing 4Ig-B7-H3 may play a critical role in the immunoendocrine network.