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Validated All-in-One™ qPCR Primer for VTCN1(NM_024626.2) Search again
Product ID:
HQP019159
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
B7-H4, B7H4, B7S1, B7X, B7h.5, PRO1291, VCTN1
Gene Description:
V-set domain containing T cell activation inhibitor 1
Target Gene Accession:
NM_024626.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
B7H4 belongs to the B7 family (see CD80; MIM 112203) of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands (e.g., CD28; MIM 186760) on T lymphocytes.[supplied by OMIM].
Gene References into function
- May participate in negative regulation of cell-mediated immunity in peripheral tissues
- Negative regulator of T cell activation. (B7S1)
- Tight regulation of B7-H4 expression at the translational level is observed in normal peripheral tissues and a potential role indicated in the evasion of tumor immunity.
- B7-H4 mRNA and protein are overexpressed in human serous ovarian cancers and breast cancers.
- B7-H4+ tumor macrophages constitute a novel suppressor cell population in ovarian cancer; B7-H4 expression represents a critical checkpoint in determining host responses to dysfunctional cytokines in ovarian cancer.
- B7-H4 in human antigen-presenting cells negatively regulates T cell responses.
- patients with renal cell carcinoma (RCC) tumors expressing both B7-H4 and B7-H1 are at an even greater risk of death from RCC
- T-cell and epithelial cell crosstalk-associated B7-H4 induces T-cell activation and is a potential activator that promotes tubular lesion
- B7-H4 expression was low in normal ovaries and in benign tumors while half of early stage and two-thirds of late stage cancers over-expressed B7-H4.
- B7-H4 is overexpressed in hyperplastic and malignant endometrial epithelium and is correlated with the T cell number associated with the tumor. B7-H4 overexpression may reflect a aggressive biologic potential and play a role in tumor immune surveillance.
- B7-H4 was expressed more often in pancreatic ductal carcinoma than was p53.
- renal cell carcinoma patients are more likely to have detectable sB7x compared with controls and higher sB7x levels correlate with advanced tumor stage