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Validated All-in-One™ qPCR Primer for TBXA2R(NM_001060.5) Search again
Product ID:
HQP017936
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
BDPLT13, TXA2-R
Gene Description:
thromboxane A2 receptor
Target Gene Accession:
NM_001060.5(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Prostaglandin endoperoxides and thromboxane A2 activate the same receptor isoforms in human platelets.
- Mapping of a ligand-binding site for the human thromboxane A2 receptor protein.
- These results suggest TXA2 receptor polymorphism strongly interacts with IL-4R alpha polymorphism as a major determinant of high serum immunoglobulin E levels in atopic dermatitis.
- The role of TXA2R 5' UTR in differential isoform expression was studied. Exons E1 & E1b associated with TXA2R transcript(s), but their expression was mutually exclusive. Major transcription initiation sites were between -115 & -92 in E1 & at -99 in E1b.
- Transcriptional activity of the 5'flanking region
- analysis of third extracellular loop of human thromboxane A2 receptor
- Thromboxane A2 (TXA2)-mediated platelet secretion and aggregation are important in thrombosis and the stable TXA2 analogue, U46619, induces two waves of platelet secretion, each of which precedes a distinct wave of platelet aggregation
- TBXA2R alpha undergoes both NO- and prostacyclin-mediated desensitization that occur through independent mechanisms involving direct PKG phosphorylation of Ser331, in response to NO, and PKA phosphorylation of Ser329, in response to prostacyclin.
- These experiments indicate that the expression of the human thromboxane A2 receptor is differently regulated in platelet precursor cells by the protein kinase A and C pathway.
- results indicate that oxidative stress induces maturation and stabilization of the thromboxane A(2)Receptor beta protein probably by intracellular translocation
- TXA2 receptors mediated concurrent platelet aggregation and shape change responses, that are differentially modulated by different signaling pathways
- A novel role is shown for isoform-specific regulation of angiogenesis by TBXA2R, providing the 1st functional significance for the existence of 2 TP isoforms in humans, & clarifying the mechanism by which TP signaling regulates FGFR1 kinetics & signaling
- Nm23-H2 had a cytoplasmic and nuclear localization but was induced to translocate to the plasma membrane upon stimulation of thromboxane A2 receptor beta to show extensive co-localization with the receptor.
- Constitutive endocytosis of thromboxane A2 receptor forms a pool of receptors in the perinuclear recycling endosome from which they recycle to the cell surface, a process involved in preserving receptor sensitivity to agonist stimulation.
- Results describe a three-dimensional structural model for the thromboxane A(2) receptor.
- TPbeta, but not TPalpha, expression is required for the inhibition of VEGF-induced migration and angiogenesis. TP stimulation appears to limit angiogenesis, at least in part, by inhibiting the pro-angiogenic cytokine VEGF.
- important physiological activators of platelets and exert their effects by acting on cell surface receptors
- prostacyclin and thromboxane receptor dimerization facilitates thromboxane receptor-mediated cAMP generation
- Thromboxane A2 receptor receptor activation causes DNA synthesis and cell proliferation of human bronchial smooth muscle cell
- the actin cytoskeleton plays an essential role in TPbeta endocytosis
- an interaction between Rab11 and TPbeta directs TPbeta recycling
- data provide direct evidence for the role of PPARgamma in the regulation of human TBXA2R gene expression within the vasculature and point to further critical differences in the modes of transcriptional regulation of TBXA2R alpha and TBXA2R beta in humans
- analysis of the molecular mechanism of how the human TXA2 receptor interacts with G alpha 13 to activate intracellular signaling
- analysis of key amino acids (in particular Asp(193)) involved in TPR ligand coordination
- analysis of functional polymorphisms of the thromboxane A2 receptor
- Food intake increases this receptor's platelet activation in type 2 diabetes but not in normal controls.
- Studies confirm that TPbeta isoform but not TPalpha is palmitoylated at Cys347, and to a lesser extent at Cys373/377 a modification that induces signalling and internalization.
- Specific single nucleotide polymorphisms and haplotypes may have utility as genetic markers for the risk of cerebral infarction.
- Results suggest that TP beta binds to alpha 7 and PA28 gamma, and the cell-surface expression of TP beta is lower than that of TP alpha.
- PRDX4 interacts with and regulates TBXA2R.
- Expression of prostaglandin E(2) receptors (EP(2), EP(3), EP(4)), prostaglandin D(2) receptor (DP(2)), prostanoid thromboxane A(2) receptor (TP) and to a lesser extent EP(1) were observed in several hair follicle compartments.
- TPr stimulation triggers reactive oxygen species-mediated LKB1-dependent AMPK activation, which in return inhibits cellular protein synthesis in VSMCs.
- These results reveal the possibly important molecular mechanisms in TP signaling and provide structural information to characterize other prostanoid receptor signalings.
- RACK1 directly binds to the C-terminus and the first intracellular loop of the beta isoform of the thromboxane A(2) receptor
- analysis of regulation of platelet responses to P2Y and thromboxane receptor activation
- TBXA2R are expressed in prostate cancer and activation of TBXA2R regulates prostate cancer cell motility and cytoskeleton reorganization through activation of RhoA.
- New roles for TPalpha and TPbeta and, through studies in aortic smooth muscle cells, reveal an additional mode of regulation of vascular smooth muscle contractile responses by TXA(2).
- studies provide a basis for the high-yield expression and purification of the G protein-coupled receptor for the structural and functional characterization using biophysics approaches
- Data suggest that expression of prostanoid receptors (prostaglandin E2 EP3-I, prostacyclin, and thromboxane A2 receptors) in vascular inflammation could influence cell responses dependent on the constitutive activation of ghrelin receptors.
- endocannabinoid 2-arachidonoylglycerol induced platelet activation with a dose-dependent mechanism that required engagement of platelet TxA(2) receptors
- Regulation of the human thromboxane A2 receptor gene by Sp1, Egr1, NF-E2, GATA-1, and Ets-1 in megakaryocytes.
- Demonstrate that isoprostanes inhibit angiogenesis via activation of the TBXA2R.
- activation of prostanoid thromboxane A(2) receptors in a Th2-dominant microenvironment might exacerbate the eosinophilic inflammation of the airways by synthesis and release of CCL11 from bronchial smooth muscle cells
- LDL concentration and degree of oxidation synergistically stimulate NO and IL-6 production, but only NO release is largely mediated by the TP receptor. LDL facilitates ET-1 release independent of concentration and degree of oxidation