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Validated All-in-One™ qPCR Primer for PTGER2(NM_000956.3) Search again
Product ID:
HQP015544
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
EP2
Gene Description:
prostaglandin E receptor 2
Target Gene Accession:
NM_000956.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- PGE(2) receptors and synthesis in human gastric mucosa: perturbation in cancer
- effects of PGE2 on monocyte-derived dendritic cells were mediated through increased cyclic adenosine monophosphate by 2 of the known PGE2 receptors, EP2 and EP4
- Activation of prostaglandin E2-receptor EP2 and EP4 pathways induces growth inhibition in human gastric carcinoma cell lines.
- Data show that down-regulation of prostaglandin E(2) receptor subtype EP(2) receptors represents a potential mechanism for neoplastic progression to an invasive phenotype.
- shear activates cyclooxygenase-2, via a c-Jun N-terminal kinase2/c-Jun-dependent pathway, which in turn elicits downstream prostaglandin EP2 and EP3a1 receptor mRNA synthesis
- Agonists of EP(1) and EP(2) significantly increased aromatase activity levels, which were decreased by the corresponding antagonists. Generally reflective of changes in aromatase protein expression and the pattern of mRNA expression.
- increased histone H3 acetylation involving the EP2 receptor, protein kinase A, CREB, and CREB binding protein is responsible for PGE(2)-induced StAR gene activation in endometriotic stromal cells.
- COX-2 induction by bradykinin in human pulmonary artery smooth muscle cells is mediated by the cyclic AMP response element through a novel autocrine loop involving endogenous prostaglandin E2, EP2 receptor, and EP4 receptor
- Human corpus cavernosum and cultured smooth muscle cells express EP1, EP2 and EP3 receptors.
- role of araomatic amino acids in i2 loop in Gs coupling
- elevated EP2 receptor expression may facilitate the PGE(2)-induced release of proangiogenic factors in reproductive tumor cells via intracellular cAMP-mediated transactivation of the EGFR and ERK1/2 pathways
- Endothelin-1-induced prostaglandin E2-EP2 and EP4 signaling regulates vascular endothelial growth factor production and ovarian carcinoma cell invasion
- PGE2 increases VEGF transcriptionally and involves the Sp-1 binding site via a cAMP-dependent mechanism involving EP2 and EP4 receptors
- a novel proinflammatory and proamyloidogenic function for the PGE2 EP2 receptor is demonstrated in a model of familial Alzheimer's disease.
- significantly reduced expression of PGE(2) receptors on nasal mucosa inflammatory leukocytes in the aspirin-sensitive compared with nonaspirin-sensitive rhinosinusitis patients
- Together, the results suggest that the overexpression of the human EP2 receptor plays a significant role in the protumorigenic action of PGE2 in mouse skin.
- prostaglandin-endoperoxide synthase 2 polymorphisms, prostaglandin-E receptor 2 polymorphisms, and C-reactive protein concentrations may have a role in atherothrombosis
- These data indicate that PGE(2) inhibits fibroblast activation in primary lung fibroblasts via binding of EP2 receptor and production of cyclic AMP; inhibition of collagen I proceeds via activation of PKA.
- These results suggest that the COX-2-PGE(2) pathway may be involved in IL-8 production in gastric epithelial cells.
- EP2 mRNA was significantly higher in normal colon tissue compared with tumor tissue.
- Selective stimulation of the EP2 receptor subtype, leading to epidermal growth factor receptor (EGFR) transactivation via protein kinase A
- participates in placentation through EVT invasion by up-regulating PGE2 production and PGE2 receptor expression in first trimester extravillous trophoblasts
- NB cells may lose responsiveness to PTGER2-mediated growth inhibition/apoptosis through epigenetic silencing of PTGER2 and/or disruption of downstream cAMP-dependent pathway during the neuroblastomagenesis.
- treatment of LS174T cells with indomethacin causes a down regulation of EP2 prostanoid receptors
- Results show co-expression of cyclooxygenase-2 and prostaglandin E2 receptors EP1, 2 and 4 in non-small cell lung cancer cells concomitant with the synthesis of PGE2.
- Role of EP2 receptor in mediating the PGE2 effect on stimulating cyst formation may have direct pharmacological implications for the treatment of polycystic kidney disease.
- Expression of prostaglandin E(2) receptors (EP(2), EP(3), EP(4)), prostaglandin D(2) receptor (DP(2)), prostanoid thromboxane A(2) receptor (TP) and to a lesser extent EP(1) were observed in several hair follicle compartments.
- PGE(2) via EP(2) and EP(4) activates the cAMP-->PKA-->CREB pathway leading to enhanced CYP19 transcription and increased aromatase activity through BRCA1 and p300
- herpes simplex virus type 1 infected mature monocyte-derived dendritic cells demonstrated a dramatic down-regulation of the expression of the EP2 and EP4 receptors
- PGE2 receptors were expressed in both NCI-H292 and human nasal epithelial cells.
- results showed that EP2 is expressed in trigeminal neurons (58% of total neurons) and is co-expressed in TRPV(1)-positive neurons (64% of TRPV(1)-positive neurons.
- CCR7 and the EP2/EP4 receptor signaling pathway are down-stream targets for COX-2 to enhance the migration of breast cancer cells toward LECs and to promote lymphatic invasion
- Prostaglandin E(2), via the prostanoid receptor EP2 and subsequent cAMP elevation, downregulates mRNA and protein levels of protease-activated receptor-1 in human lung fibroblasts.
- PTGER2 methylation was present with greater frequency in nonsmall cell lung cancer with EGFR mutation.
- A new approach for blocking E-prostanoid receptor-mediated cell signaling using a soluble chimeric EP2 fragment, is described.
- findings show the G(s)-coupled EP(2) receptor closes K(Ca)3.1 in lung mast cells and attenuates both chemokine- and PGE(2)-dependent lung mast cell migration
- PGE(2) induces angiogenesis in prostate cancer through EP2 and EP4.
- Common polymorphisms of cyclooxygenase-2 and prostaglandin E2 receptor and increased risk for acute coronary syndrome in coronary artery disease.