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Validated All-in-One™ qPCR Primer for PDGFB(NM_002608.3) Search again
Product ID:
HQP012856
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis
Gene Description:
platelet derived growth factor subunit B
Target Gene Accession:
NM_002608.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq].
Gene References into function
- The sequential activation and repression of the human PDGF-B gene during chronic hypoxia reveals antagonistic roles for the depletion of oxygen and glucose
- Chlamydia pneumoniae infection of endothelial cells induces transcriptional activation of platelet-derived growth factor-B: a potential link to intimal thickening in a rabbit model of atherosclerosis (PDGFF-B)
- Fusion of COL1A1 exon 29 with PDGFB exon 2 in a der(22)t(17;22) in a pediatric giant cell fibroblastoma with a pigmented Bednar tumor component. Evidence for age-related chromosomal pattern in dermatofibrosarcoma protuberans and related tumors.
- A fusion of COLIA1 exon 24 in frame with PDGFB exon 2 was found in a complex marker chromosome from dermatofibrosarcoma protuberans with sequences from chromosomes 7,8,17,21, & 22.
- determination of PDGFB expression levels in medulloblastoma tumor cell lines
- involvement of Furin gene regulation in the maturation of PDGF-AB in differentiating megakaryoblastic cells
- PDGF and similar cytokines may be important factors in airway remodeling by redistribution of smooth muscle cells during inflammation and that urokinase may be important in potentiating the response.
- The preferential formation of disulfide-bonded heterodimers from an equimolar mixture of unfolded A- and B-chains is thus a kinetically controlled process
- PDGFB enhances wound closure in the absence of wound contraction.
- a time-dependent release of TGFB and PDGF-AB occurred at neutral and alkaline pH, but not pH5, 15 min and 12h after platelet activation, suggesting relevance to wound healing
- propose that the TATA-containing P0 promoter and the 5'-UTR promoter work together to tightly control the expression of PDGF-B
- role in dermal fibroblast migration
- The COL1A1-PDGFB fusion transcripts were detected from the tumor specimens. Sequence analysis revealed that the ends of exons 42, 29 and 38 in the COL1A1 gene were fused with the start of exon 2 in the PDGFB gene in case 1, 2 and 3, respectively.
- cultured follicular keratinocytes synthesize both PDGF-A and PDGF-B, whereas, dermal papilla cells only express PDGF-A.
- PDGF-BB may play some role in the development and progression of liver fibrosis
- PDGFB autocrine inhibition is associated with malignant gliomas
- biological consequences of the mutant PDGFB allele contributed to the unique disease phenotype of the translocation patient
- Overexpression of caveolin-1 in mesangial cells suppresses MAP kinase activation and cell proliferation induced by bFGF and PDGF, two major cytokines in mesangioproliferative nephritis(MN). Caveolin-1 expression vector is potential therapy for MN.
- Data demonstrate that type I collagen synergistically enhances platelet-derived growth factor (PDGF)-induced smooth muscle cell proliferation through Src-dependent crosstalk between the alpha2beta1 integrin and the PDGF receptor beta.
- Data show that constitutive expression of platelet-derived growth factor-BB leads to malignant transformation in nude mice.
- WAVE3-mediated migration in MDA-MB-231 cells via lamellipodia formation is activated downstream of PI3K and induced by PDGF
- NAD(P)H oxidase is expressed in hepatic stellate cells and produces reactive oxygen species via activation of NAD(P)H oxidase in response to the homodimeric complex of PDGF-B, PDGF-BB.
- EWS-Fli1 may play a role in the regulation of PDGF-induced tumor proliferation-signaling enzymes via PLD2 expression in Ewing sarcoma cells
- In the aneurysmal wall of IAAA, weak expressions of PDGF A and B chains were observed in endothelial cells of vessel walls around the inflammatory cells, but the intensity of expression was much weaker than that on the vessel walls in AAAA.
- findings emphasize the importance of the convertase-directed processing of proPDGF-B at the RGRR81/ sequence for PDGF-B maturation and secretion
- in primary fibroblasts stabilization of Ras protein by ROS and ERK1/2 amplifies the response of the cells to growth factors and in systemic sclerosis represents a critical factor in the onset and progression of the disease
- PDGF-BB/PDGF-R interaction is vital in nicotine's mitogenic actions on human aortic smooth muscle cells
- Expression of PDGF-B and PDGF-Rbeta suggests that autocrine signaling of PDGF may be important in malignant transformation of B-CLL.
- PDGF-B gene polymorphism appears to be associated with severe recurrent HCV infection after liver transplantation.
- Overexpression of PDGF-B was associated with peripheral blood, sentinel lymph node and bone marrow micro-metastasis in breast cancer
- PDGF-BB and TGF-beta1 binding to intact alpha2M is specific, involving a defined region of the alpha2M subunit
- In this review, the interaction of PDGF-B with heparan sulfate proteoglycans is implicated in the process of diffusion, and thus the availability, of PDGF-B that is needed for pericyte recruitment around newly formed capillaries.
- report suggests that in humans, platelet-derived growth factor(PDGF) and downstream activation of protein kinase C could represent an important control for natriuretic peptide precursor C expression in vascular smooth muscle
- Fluorescence in situ hybridization (FISH) analysis demonstrated the COL1A1-PDGFB fusion on both der(22) chromosomes. [Col1A1-PDGFB fusion protein]
- PDGF significantly upregulates neuropilin-1 mRNA expression and protein production in aortic smooth muscle cells
- GRX plays an important role in PDGF-BB-dependent cell proliferation by regulating the redox state of LMW-PTP
- The mRNA levels of PDGFB were significantly produced by the megakaryocytes may be associated with the etiology of bone marrow fibrosis in AMM.
- Copy gain of PDGFB occurs in a subset of tumors showing no evidence of mutated BRAF or rearranged ret, suggesting that copy gain of PDGFB may underlie the increased expression of platelet-derived growth factor described recently in the literature.
- N-cadherin and beta-catenin play role in cell migration via PDGF-Rbeta-mediated signaling through the scaffolding molecule NHERF2
- Induction of PDGF-B is associated with glioma
- An unexpected decrease in fibroblast matrix stiffness occurred in response to rhPDGF-BB.
- In absence of serum, it required a combination of Ang-2 and PDGF-BB to increase invasion. The heightened invasion was dependent on serine proteases and MMPs, combination of Ang-2 and PDGF-BB increased urokinase plasminogen-activator receptor expression.
- The presence of PDGFB and PDGFRB mRNAs was demonstrated in 26 and 21 of 26 cases dermatofibrosarcoma protuberans, respectively.
- In summary, the data demonstrate complex gene-regulation mechanisms between endothelial cells and osteoblasts that are likely to play a role in bone morphogenesis.
- KIT and PDGFRbeta gatekeeper mutations are involved in tumor progression and are resistant to imatinib, but are inhibited by sorafenib
- Er:YAG laser used alone or in combination with rhPDGF-BB application may offer a promising periodontal therapy for conditioning root surfaces, although the combined application seemed to be slightly more effective.
- These findings indicate that the development of restenosis depends on both complement activation regulated by the MBL2 gene and pathologic processes leading to enhanced production of VEGF and PDGF during the very early postoperative period.
- endothelial and lung cancer cells seem to interact via various paracrine pathways, e.g. by the reciprocal induction of VEGF and PDGF-BB
- The trichloroacetic acid-treated epidermis acts as a major source of growth factors, including PDGF-B, before undergoing full necrosis.
- Increased expression of PDGF/Ralphabeta may play an important role in the mechanism of growth of...paediatric fibromatous lesions
- we found COL1A1-PDGFB rearrangements appear more prevalent in Dermatofibrosarcoma protuberans (DFSP) than previously reported.
- thrombin may play an important role in the proliferation of A172 cells by inducing PDGF-AB secretion and that thrombin's action is mediated by its proteolytic activity
- ERK5 modulates platelet-derived growth factor-dependent biologic activities in human hepatic stellate cells
- Genomic gains of COL1A1-PDGFB were found predominantly in the DFSP component of GCF/DFSP hybrids but in none of the pure GCF
- In PDGFR-expressing immortalized ovarian cancer cells, PDGF strongly induced VEGF secretion, suggesting a correlation between the PDGF & VEGF networks in ovarian cancer.
- Limiting Cx43 expression in vascular smooth muscle cells prevents platelet-derived growth factor-BB-induced phenotypic modulation.
- PDGF-BB reduced gp120-associated neurotoxicity, rescued neurite outgrowth, & reduced gp120-mediated caspase-3 activation. It increased the Bcl-xL to Bax ratio. PDGF-mediated protection against gp120 involved the phosphoinositide 3-kinase/Akt pathway.
- Significantly higher level of PDGF-B is associated with papillary thyroid cancer
- platelet-derived growth factor-BB plasma levels with kidney allograft rejection
- The proteins for PDGF-B were detected in oocytes, and in granulosa cells (GC) of 50% of the follicles from women/girls.
- Document a role for FGF-2 and PDGF-BB in the temporal regulation of coronary artery stem formation and growth of the coronary arterial tree.
- Chronic insulin treatment amplifies PDGF-BB induced motility in differentiated aortic smooth muscle cells by suppressing the expression and function of PTP1B.
- PDGF-BB expression may be a new prognostic indicator for patients with colorectal cancer
- Results reveal that platelet-derived growth factor-B-activated fibroblasts play a key role in the recruitment/migration and differentiation of mesenchymal stem cells and implicate a bFGF- and CXCL5-dependent mechanism in mediating these effects.
- May induce coronary atherosclerotic disease formation by stimulating coronary artery smooth muscle cell migration and proliferation.
- Akt1/PKBalpha controls cell migration by selectively translocating to the leading edge and activating Rac.
- High tumor cell PDGF-B is associated with non-small cell lung cancer.
- Neuraminidase caused the desialylation of both PDGF and IGF-1 receptors and diminished the intracellular signals induced by the mitogenic ligands PDGF-BB and IGF-2.
- migration of osteoblasts is controlled by the balance between PDGF, S1P and the differentiation state of the cells
- PDGF-mediated protection also involved the inhibition of gp120-induced release of mitochondrial cytochrome C. Our findings thus underscore the roles of both PI3K/Akt and Bcl family pathways in PDGF-mediated neuroprotection.