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Validated All-in-One™ qPCR Primer for HTR1B(NM_000863.2) Search again
Product ID:
HQP009116
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1DB, HTR1D2, HTR1DB, S12
Gene Description:
5-hydroxytryptamine receptor 1B
Target Gene Accession:
NM_000863.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) exerts a wide variety of physiologic functions through a multiplicity of receptors and may be involved in human neuropsychiatric disorders such as anxiety, depression, or migraine. These receptors consist of 4 main groups, 5-HT-1, 5-HT-2, 5-HT-3, and 5-HT4, subdivided into several distinct subtypes on the basis of their pharmacologic characteristics, coupling to intracellular second messengers, and distribution within the nervous system (Zifa and Fillion, 1992 [PubMed 1359584]). The serotonergic receptors belong to the multigene family of receptors coupled to guanine nucleotide-binding proteins.[supplied by OMIM].
Gene References into function
- Genetic variability of the 5HT1B receptor is involved in the development of some type of alcohol dependence.
- a potential susceptibility locus at the 5-HT(1B) receptor gene in attention deficit hyperactivity disorder
- substance abuse disorder and major depression appear to be associated with the 5-HT1B receptor G861C locus in the patient population, but other psychopathologies such as bipolar disorder, schizophrenia, alcoholism, and suicide attempts were not
- may be a good candidate for genetic studies of ADHD.
- G861C, G261T, and C129T are not playing any direct role in the development of susceptibility to alcoholism
- Population-based association study tested the hypothesis that the serotonin 1B receptor (HTR1B) A-161T polymorphisms was associated with TPQ personality trait scores in a sample population of 209 young healthy Chinese.
- HTR1B is the target of substantial transcriptional genetic regulation by common haplotypes, which are in linkage disequalibrium with the HTR1B single-nucleotide polymorphism (SNP) most commonly used in association studies.
- Based on the examination of 164 alcoholic subjects, an association is found between a lower frequency of the 5-HT1B 861cytosine allele, antisocial personality traits and conduct disorder in alcohol-dependent subjects.
- Alterations in 5-HT(1A,) 5-HT(1B), and 5-HT(2A) mRNA levels in the brains of subjects with both mood disorders and schizophrenia support the hypothesis of dysregulation of the serotonergic system in these psychiatric disorders.
- data provide no support for the hypothesis that polymorphisms at 5-HT1Dalpha (TaqI) and 5-HT1Dbeta (T261G and G861C) genes contributes to susceptibility to schizophrenia in the Portuguese population
- Not significantly associated with aggression. (5HT1Dbeta receptor)
- Evidence is provided for mismatch of 5-HT1B receptor mRNA expression and receptor distribution in human brain, giving support to species differences in the cortical mRNA expression and localization of this receptor subtype.
- this is the first association study to suggest how a particular gene might influence OCD pathology in an eating disorder population.
- Postsynaptic 5-HT(1B) receptors directly influence the executive, consummatory phases of agonistic behavior, whereas presynaptic serotonergic feedback systems are particularly useful in the introductory phases of the agonistic behavioral complex.
- results of the present study indicate that the dynamic modulation of 5-HT1B receptor function by p11 may be involved in molecular adaptations occurring in neuronal networks that are dysfunctional in depression-like states
- The contribution of 5-HT1B receptors to the mediation of the effects of 5-HT is increased in 124Cys/Phe individuals.
- lack of association of obsessive behaviors/perfectionism and the HTR1B gene in a sample of 203 families with an ADHD proband
- antagonism of the 5-HT(1B) receptor inhibits the proliferation of both human and murine primary helper T cells and of human helper T cell lines
- investigate seven genetic variants in three genes (serotonin transporter (5-HTT), serotonin receptor 1B (5-HTR1B) and serotonin receptor 2A (5-HTR2A)), which have previously been shown to be associated with ADHD
- Genetic variation of the serotonin 1B receptor gene is associated with impulsive aggressive behavior and suicide
- there was no significant association between the HTR1B G861C polymorphism and suicidal behavior (Meta-Analysis)
- a G-G haplotype (rs1213368-rs6296) and the respective G-alleles were found to be related to a strong loudness dependence of auditory evoked potentials response of the left tangential dipole, indicating low serotonergic activity
- Examination of genetic variants at the HTR1B locus indicated no association between the selected polymorphisms and childhood-onset mood disorder.
- In the human internal thoracic artery, 5-hydroxytryptamine (5-HT)-induced vasoconstriction is mediated by activation of both 5-HT2A and 5-HT1B receptors.
- findings showed evidence of undertransmission of the HTR1B haplotypes containing alleles -161G and -261A at HTR1B gene to autism spectrum disorders (ASD) (P=0.003), but no involvement of HTR2C to the predisposition to ASD