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Validated All-in-One™ qPCR Primer for SIGLEC7(NM_014385.2) Search again
Product ID:
HQP007456
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
AIRM-1, AIRM1, CD328, CDw328, D-siglec, QA79, SIGLEC-7, SIGLEC19P, SIGLECP2, p75, p75/AIRM1
Gene Description:
sialic acid binding Ig like lectin 7
Target Gene Accession:
NM_014385.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Gene References into function
- Regulation of myeloid cell proliferation and survival by p75/AIRM1 and CD33 surface receptors.
- high resolution crystal structures of this protein; insights into ligand specificity of this family of proteins
- Sialidase unmasking of siglec-7 on NK cells permits it to interact with GD3 on target cells and inhibit killing. This has inplications for tumor escape from NK cells.
- x-ray crystallographic analysis of the saccharide-binding domain of p75/AIRM1
- Siglec-7 and Siglec-9 are capable of negative regulation of TCR signaling and ligand binding is required for optimal activity
- Siglec-7 can inhibit Fc epsilon receptor I-mediated serotonin release from rat basophilic leukemia cells and recruit the tyrosine phosphatases SHP-1 and SHP-2.
- upon pervanadate (PV) treatment, Siglec-7 recruited the protein tyrosine phosphatases Src homology-2 (SH2) domain-containing protein-tyrosine phosphatase-1 and SHP-2 less efficiently than did other inhibitory receptors
- structural analysis of siglec-7 in complex with sialosides
- Siglecs-7 did not interact with sulfate derivatives of LacNAc and sulfated oligosaccharides containing sialic acid.
- The expression levels of Siglec-7 were detectable in bone marrow plasma from AML patients and serum from normal donors.
- The species-specific differences in the expression expression of Siglecs in SIV infection was studied.