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Validated All-in-One™ qPCR Primer for CNR2(NM_001841.2) Search again
Product ID:
HQP002412
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
CB-2, CB2, CX5
Gene Description:
cannabinoid receptor 2
Target Gene Accession:
NM_001841.2(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq].
Gene References into function
- CB1 and CB2 receptor mRNA expression in human peripheral blood mononuclear cells (PBMC) from various donor types.
- dendritic cells were also found to express measurable amounts of CB1 and CB2 receptors and of FAAH. Cell maturation did not consistently modify the expression of these proteins
- Absence of a conserved proline and presence of a conserved tyrosine in the CB2 cannabinoid receptor are crucial for its function.
- CB2 plays a role in inhibiting neovascularization and skin neoplasm development
- Effects of D3.49A, R3.50A, and A6.34E mutations on ligand binding and activation of the cannabinoid-2 (CB2) receptor.
- 2-arachidonoylglycerol induces the migration of several types of leukocytes such as macrophages/monocytes through a CB2 receptor-dependent mechanism thereby stimulating inflammatory reactions and immune responses
- analysis of human acute myeloid leukemia (AML) samples revealed the presence of CB2 mRNA transcripts in several cases.
- In hippocampus and entorhinal cortex of Alzheimer's disease patients both fatty acid amide hydrolase and cannabinoid CB2 receptors are abundantly and selectively expressed in neuritic plaque-associated astrocytes and microglia
- A critical role is indicated for CB2 in migration of B cells and the lymphoid germinal center response.
- delta(9)-THC induces an influx of extracellular calcium in resting T cells in a CB1- CB2- -dependent manner
- aberrantly expressed in a high percentage of human acute myeloid leukemias
- CB2 receptors are present in a specific microglial cell type of the human cerebellum, namely, perivascular microglial cells.
- proposition that 2-AG (2-arachidonoylglycerol) is the true natural ligand for both the CB1 and CB2 receptors
- The immune system-associated cannabinoid CB2 receptors were localized only to placental macrophages
- Senile plaques in AD patients express CB2 receptors which show increased nitration.
- CB2 receptors highly up-regulated in cirrhotic liver, predominantly in hepatic fibrogenic cells. Antifibrogenic role of CB2 receptors during chronic liver injury.
- Collectively, these results demonstrate reduced endogenous fatty acid amide immunomodulatory responses in individuals with the CB2 188-189 GG/GG genotype and suggest that this CB2 gene variation may be a risk factor for autoimmunity.
- CB1 and CB2 immunoreactivity was observed in cutaneous nerve fiber bundles, mast cells, macrophages, epidermal keratinocytes, and the epithelial cells of hair follicles, sebocytes and eccrine sweat glands.
- Data support a role for p38 MAPK in cannabinoid receptor 2-induced apoptosis of human leukaemia cells.
- A role is demonstrated for the peripherally expressed CB2 receptor in the etiology of osteoporosis, whereas no convincing association is found for cannabinoid receptor type 1 (Cnr1).
- Results describe the modification of 12-phenylacetyl-ricinoleoyl-vanillamide and its activity at TRPV1 and CB2 receptors.
- CB(2) receptors are involved in cannabinoid-mediated inhibition of the chemokine CXCL12-induced and CXCR4-mediated chemotaxis of Jurkat T cells and their transendothelial migration.
- CB2 might play a role in regulating excessive inflammatory response by controlling RhoA activation, thereby suppressing neutrophil migration
- analysis of cannabinoid type 2 receptor-dependent and -independent immunomodulatory effects of alkylamides from Echinacea
- These data strongly suggest that AM1241 produces antinociception in vivo by activating CB2 cannabinoid receptors.
- Results presented here show that CB2 receptor activation signals apoptosis via a ceramide-dependent stimulation of the mitochondrial intrinsic pathway.
- lipid rafts control CB1R, but not CB2R, and endocannabinoid transport in immune and neuronal cells.
- apoptosis induced by cannabinoid receptor CB1 and CB2 agonists leads to activation of ERK1/2 leading to G1 cell cycle arrest in prostate cancer cells
- High expression of CB2 receptor was observed in 33 (52%) hepatocellular carcinoma.
- There is an association between the Q63R polymorphism of the CB2 gene and alcoholism in a Japanese population.
- We tested if cannabinoid type 2 receptor (CB2) in the central nervous system plays a role in alcohol abuse/dependence in animal model and then examined an association between the CB2 gene polymorphism and alcoholism in human.
- Dual affinity tags were used for the expression and purification of functional CNR2.
- Activation of CB2 cannabinoid receptors by JWH133 protects against I/R damage by decreasing inflammatory cell infiltration, tissue and serum TNF-alpha, MIP-1alpha and MIP-2 levels, tissue lipid peroxidation, and expression of ICAM-1 in vivo
- polymorphisms of CNR2 may confer susceptibility to postmenopausal osteoporosis in women, and that of GJA4 to osteoporosis in men
- abundant levels of CB2 protein were present on T-NHL and in many B-NHL. NHL specimens in general stained positively with both C-terminal specific anti-CB2 and N-terminal specific CB2 antibody
- CB2 receptor agonists attenuated TNF signaling in inflammation models.
- CB(1)R and CB(2)R are differentially linked to lipid rafts, specialized microdomains of the plasma membrane--REVIEW
- insulin may play a key role in the obesity-linked dysregulation of the adipose endocannabinoid system at the gene level
- CB(2) receptors are induced in beta-amyloid plaque-associated microglia and astroglia, respectively, in Down's syndrome
- CB2 is densely present in somatostatin-secreting pancreatic delta cells.
- Circular dichroism spectroscopy indicated high alpha-helical content (49 %) of CB2
- high incidence of (Q63R) but not (H316Y) polymorphism in the CB2 gene was found in Japanese depressed subjects
- support the potential use of non-psychoactive CB(2) agonists in therapeutic strategies for multiple sclerosis and other neuroinflammatory disorders
- Selective CB2 up-regulation in women affected by endometrial inflammation is reported.
- cannabinoid receptors are required for ultraviolet-induced inflammation and skin cancer development
- temporal-spatial expression of FAAH, CB1, and CB2 is regulated during early pregnancy and anandamide detrimentally alters trophoblast proliferation
- Increases the susceptibility of macrophages to oxidized low density lipoprotein-induced apoptosis.
- Data suggest that common polymorphisms within the cannabinoid receptor 2 gene confer no susceptibility to myocardial infarction or to cardiovascular risk factors.
- fibroblast-like synoviocytes of knees of rheumatoid arthritis and osteoarthritis patients express cannabinoid receptor (CB)1 and CB2
- Data demonstrate the functional expression of CB2 receptors in the brain that may provide novel targets for the effects of cannabinoids in depression and substance abuse disorders beyond neuroimmunocannabinoid activity.
- demonstrate that side chains of the residues at these reactive loci (V6.51, L6.52, L6.54, M6.55, L6.59, and T6.62) are on the water-accessible surface of the binding-site crevice
- study demonstrates that expressed cannabinoid CB(2) receptors couple equally well to Galpha(qo5) and Galpha(qi5) proteins