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Validated All-in-One™ qPCR Primer for CCR3(NM_001837.3) Search again
Product ID:
HQP002207
(click here to view gene annotation page)
Powered by BiozSpecies:
Human
Symbol:
Alias:
C C CKR3, CC-CKR-3, CD193, CKR 3, CKR3, CMKBR3
Gene Description:
C-C motif chemokine receptor 3
Target Gene Accession:
NM_001837.3(click here to view gene page)
Estimated Delivery:
Approximately 1-3 weeks, but may vary. Please email sales@genecopoeia.com or call 301-762-0888 to confirm ETA.
Important Note:
By default, qPCR primer pairs are designed to measure the expression level of the splice variant (accession number) you selected for this gene WITHOUT consideration of other possible variants of this gene. If this gene has multiple variants, and you would like to measure the expression levels of one particular variant, multiple variants, or all variants, please contact us for a custom service project at inquiry@genecopoeia.com.
Validated result:
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| A | B |
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Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve. |
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Summary
The protein encoded by this gene is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1. This gene and seven other chemokine receptor genes form a chemokine receptor gene cluster on the chromosomal region 3p21. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq].
Gene References into function
- agonistic effect of truncated leukotactin-1 on receptor activity
- Alanine scanning mutagenesis of CCR3 reveals that the three intracellular loops are essential for functional receptor expression.
- bronchial expression up-regulated in exacerbations of chronic bronchitis
- demonstrated that CCR3 is absent from the testes
- downstream promoter element dependent, general transcription control mechanism is conserved between Drosophila and human genes
- CCR3 is expressed by dendritic cells (DCs) differentiated from blood monocytes, DCs that emigrate from skin (epidermal and dermal DCs), and DCs derived from CD34+ hemopoietic precursors in bone marrow, umbilical cord blood, and leukapheresis collection.
- results suggest that expression of chemokine receptor 3(CCR3) and its ligand eotaxin/CCL11 plays a role in the recruitment and retention of CD30(+) malignant T cells to the skin
- This protein mobilizes to the surface of human mast cells and potentiates immunoglobulin E-dependent generation of interleukin 13.
- Eosinophil CCR3 expression between atopic individuals does not correlate with atopy or serum IgE levels, nor is the status of macrophage-inflammatory protein-1alpha-highly responsive donors associated with increased chemattractant receptor expression.
- eosinophil responses mediated by chemokines acting at CCR3 may be regulated by two distinct mechanisms: the antagonistic effects of CXCR3 ligands and the sequestration of CCL11 by CXCR3-expressing cells
- Ligation of CCR3 by eotaxin/chemokine ligand (CCL) 11 induces apoptosis in IL-2- and IL-4-stimulated primary CD19+ B cell cultures (approximately 40% apoptotic cells) as well as B cell lines, but has no effect on chemotaxis or cell adhesion.
- Single nucleotide polymorphism is not associated with atopic dermatitis in Japanese.
- the two N-terminal motifs of eotaxin must cooperate with other regions to successfully bind and activate CCR3
- examination of the role of epidermal growth factor receptor in CCR3 signaling
- multiple residues on multiple extracellular domains of hCCR3 are important for coreceptor activity for HIV-1.
- the restoration of CRTH2(chemoattractant receptor-homologous molecule expressed on Th2 cells)/CCR3 expression may be an indicator for optimal recovery after septic shock
- The mouse transcription factor GATA-1 binds to transgenic human CCR3 exon 1. The 1.6-kb CCR3 promoter element, that includes exon 1, is a strong promoter in vivo.
- Up-regulation of CCR3 by airway smooth muscle cells in asthmatic subjects might be partly responsible for the increased smooth muscle mass and the airway hyperresponsiveness in these patients.
- CCR5 and CCR3 receptors are expressed on the head region of human spermatozoon
- Epithelial CCR3 participates in key functions for wound repair
- Chemokine (C-C-motif) receptor 3 (CCR3), playing an important role in endometrium related metabolic pathways, may influence the onset of menarche.
- This study finding provide a strong evidence that Eotaxin 1 Thr23Thr homozygote has a protective effect on asthma and significantly decreases plasma Eotaxin 1 concentrations in asthmatics in Taiwan.
- The genetic association between CCR3 polymorphisms and the number of circulating eosinophils was revealed;it was more pronounced when the CCR3 polymorphisms were paired with polymorphisms in IL5RA.
- The allergen-induced levels of beta(c) mRNA and CCR3 mRNA in sputum-derived cells were inhibited by TPI ASM8, with no significant effects on the cell surface protein expression of CCR3 and beta(c).
- infiltrated neutrophils from patients with chronic inflammatory lung diseases and rheumatoid arthritis highly express CCR1, CCR2, CCR3, CCR5, CXCR3, and CXCR4