Validated All-in-One™ qPCR Primer for KIR3DL3(NM_153443.4) Search again

Buy	Catalog#	Description	Note	Price (US$)
	HQP001810	All-in-One™ qPCR Primer for NM_153443.4 (200 reactions)	KIR3DL3	inquire
	HQP006940	All-in-One™ qPCR Primer for NM_002046.6 (200 reactions)	Reference Gene GAPDH	inquire
	HQP006940	All-in-One™ qPCR Primer for NM_002046.7 (200 reactions)	Reference Gene GAPDH	inquire
	HQP015171	All-in-One™ qPCR Primer for NM_004048.3 (200 reactions)	Reference Gene B2M	inquire
	HQP015171	All-in-One™ qPCR Primer for NM_004048.4 (200 reactions)	Reference Gene B2M	inquire
	HQP016381	All-in-One™ qPCR Primer for NM_001101.5 (200 reactions)	Reference Gene ACTB	inquire

Validated result:

A	B
Figure: All-in-One™ qPCR Primer Validated result to KIR3DL3. Each All-in-One™ qPCR Primer is experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted gene. A cDNA Pool, containing reverse transcript products of 8 different human tissue total RNA(Liver, Testis,Muscle,Thyroid,Brain,Spleen,Stomach,Small Intestine)),was used as the qPCR validation template. qPCR was performed using 0.2 µM primer with 2XAll-in-One™ qPCR Mix (Catalog#: QP001,QP002,QP004,QP005). Reactions were incubated for 10min. at 95°C, followed by 40 cycles of 95°C for 10 sec.; 60°C, 20 sec. and 72°C, 15sec. using Bio-Rad iQ5™ Instrument. At the end of the last cycle, temperature was increased from 72 to 95°C to produce a melting curve. Panel A: Validated Result for Amplification Curve; Panel B: Validated Result for Melting Curve.

Summary

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several 'framework' genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. This gene is one of the 'framework' loci that is present on all haplotypes. [provided by RefSeq].

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