GeneCopoeia’s GLuc-ON™ PKC transcriptional response element (TRE) lentiviral clone enables analysis of the effects on genes regulated by activator protein 1 (AP1). AP1 is a transcription factor complex comprised of the c-Fos and c-Jun family proteins, and is necessary for signaling through the Protein kinase C (PKC) pathway.
The GLuc-ON™ PKC TRE lentiviral clone contains tandem repeats of PKC upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The PKC response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PKC TRE lentiviral clone is provided as transfection-ready DNA. A negative (non-activating) control lentiviral clone is also available for purchase.
Validation Data
Figure 1. Activation of the GLuc-ON™ PKC TRE by phorbol 12-myristate 13-acetate (PMA). HEK293a cells were transduced with negative control lentiviral particles or the GLuc-ON™ PKC TRE lentiviral particles. Transduced cells were incubated for 48 hours, followed by 6 days of incubation in the presence of puromycin. Puromycin-resistant cells were then treated with PMA for 18 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as the fold activation of luminescence.