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EndoFectin™ HepG2 Transfection Reagent

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EndoFectin™ HepG2 Transfection Reagent

EndoFectin™ HepG2 is a new and potent reagent optimized for transfection of the difficult-to-transfect hepatocellular carcinoma cell line HepG2.  EndoFectin™ HepG2 provides superior transfection efficiency of HepG2 cells compared with Lipofectamine® 3000 (Figure 1) and Lipofectamine® 2000 (Figure 2).

Advantages

  • Specially formulated for the difficult-to-transfect hepatocellular carcinoma cell line HepG2
  • Greater transfection efficiency of HepG2 cells compared with Lipofectamine® 3000  (Figure 1).
  • Superior transfection efficiency of HepG2 cells compared to Lipofectamine® 2000 (Figure 2)
  • Transfection efficiency of HepG2 cells is comparable to electroporation, allowing you to achieve ultra-high levels of transfection more conveniently (Figure 3).
  • Does not require removal of serum or culture medium or removal of medium after transfection.
 
Buy Catalog # Product Price (US$) Protocol MSDS
EF005 EndoFectin™ HepG2 transfection reagent (1 mL) $369 Download Download
EF006 EndoFectin™ HepG2 transfection reagent (3 mL) $733
 
 

Experiment 1: Comparison of EndoFectin™ HepG2 and Lipofectamine® 3000

Figure 1. Transfection of HepG2 cells using either EndoFectin™ Max, Lipofectamine® 2000 (LP2000) or Lipofectamine® 3000 (LP3000). Cells were transfected with a firefly luciferase-expressing plasmid. Transfection efficiencies were assessed by luciferase activity (RLU). Note: 0.15 uL/well of Lipofectamine® 3000 was used rather than 0.2 uL/well. Lipofectamine® is a trademark of Invitrogen.
 

Experiment 2: Comparison of EndoFectin™ HepG2 and Lipofectamine® 2000
Figure 2. Comparison of transfection efficiencies of HepG2 cells between EndoFectin™ HepG2 and Lipofectamine® 2000 (LP2000). Top: Cells were transfected with a plasmid expressing luciferase, and quantitated in a luminometer. Bottom: HepG2 cells were transfected with a plasmid expressing GFP, and visualized using either visible light or fluorescence.  

Experiment 3: Comparison of EndoFectin™ HepG2 and electroporation

EndoFectin™ HepG2 can also provide nearly as high transfection efficiency as electroporation, meaning that you can achieve ultra-high levels of transfection with much more convenience (Figure 3).
 

Figure 3. Comparison of transfection efficiencies of HepG2 cells between EndoFectin™ HepG2 and electroporation. HepG2 cells were electroporated with a plasmid expressing GFP, and visualized using either visible light or fluorescence.
 
Note: Lipofectamine® is a registered trademark of Invitrogen
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