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Lentiviral ORF,promoters,shRNA,precusor microRNA,microRNA inhibitor clones,sgRNA and Cas9 nuclease clone.

Introduction

GeneCopoeia offers the largest collection of lentiviral vector-based expression clones for ORF cDNAspromotersshRNAsprecursor microRNAs, and microRNA inhibitors.

In addition to the lentiviral clones, GeneCopoeia also provides optimized packaging plasmids, cell lines, lentivirus concentration solution, as well as high quality lentiviral packaging services as a part of a comprehensive suite of lentiviral solutions.

 
   

 

Advantages of lentiviral ORF cDNA, promoters, shRNA, and microRNA clones

High efficiency of gene delivery to virtually all cell types and whole model organisms

A lentiviral system is very effective at delivering genetic material to whole model organisms and almost all mammalian cells, including non-dividing, inactive or growing, and difficult-to-transfect cells including neuron, primary and stem cells. The efficiency of lentiviral transduction is close to 100%, making it ideal as an expression vector system.

High expression levels of delivered genes

Lentiviral expression vectors with N-or-C terminal fusion tag or un-tagged consist of sequence features and elements allowing efficient packaging, transduction and stable integration into genomic DNA of target cells, thereby enabling high levels of expression and detection of an ORF insert in the target cells.

Self-inactivation and no unwanted viral replication

OmicsLink™ ORF lentiviral expression clones are self-inactivating. Transduced cells cannot produce additional viral particles since these cells do not contain the genes needed to produce the viral capsid. Furthermore, upon integration into the target cell's genome, the 5' LTR promoter is inactivated, preventing replication of the viral sequences.

 

 

 

 

 

 

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ORF

Browse all ORF lentiviral expression vectors and search for your clone of interest. Click to learn more about OmicsLink™ expression-ready ORF clones

 

ORF lentiviral expression vectors (HIV)

 

 

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Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Lv105 CMV Stem/primary/others* Puromycin N/A N/A
pReceiver-Lv151 CMV Stem/primary/others* Neomycin N/A N/A
pReceiver-Lv152 CMV Stem/primary/others* Hygromycin N/A N/A
pReceiver-Lv153 CMV Stem/primary/others* N/A N/A N/A
pReceiver-Lv156 EF1a Stem/primary/others* Puromycin N/A N/A
pReceiver-Lv186 CMV Stem/primary/others* N/A C-3xHA N/A
pReceiver-Lv157 CMV Stem/primary/others* Neomycin C-3xHA N/A
pReceiver-Lv120 CMV Stem/primary/others* Puromycin C-3xHA N/A
pReceiver-Lv108 CMV Stem/primary/others* Puromycin N-Avi N/A
pReceiver-Lv124 CMV Stem/primary/others* Puromycin C-Avi N/A
pReceiver-Lv242 CMV Stem/primary/others* Puromycin C-Avi-FLAG N/A
pReceiver-Lv154 CMV Stem/primary/others* Neomycin C-eCFP N/A
pReceiver-Lv127 CMV Stem/primary/others* Puromycin C-eCFP N/A
pReceiver-Lv109 CMV Stem/primary/others* Puromycin N-eCFP N/A
pReceiver-Lv103 CMV Stem/primary/others* Puromycin N-eGFP N/A
pReceiver-Lv122 CMV Stem/primary/others* Puromycin C-eGFP N/A
pReceiver-Lv182 CMV Stem/primary/others* Neomycin N-eGFP N/A
pReceiver-Lv183 CMV Stem/primary/others* Neomycin C-eGFP N/A
pReceiver-Lv189 CMV Stem/primary/others* N/A N-eGFP N/A
pReceiver-Lv123 CMV Stem/primary/others* Puromycin C-eYFP N/A
pReceiver-Lv104 CMV Stem/primary/others* Puromycin N-eYFP N/A
pReceiver-Lv187 CMV Stem/primary/others* N/A C-FLAG N/A
pReceiver-Lv101 CMV Stem/primary/others* Neomycin N-Flag N/A
pReceiver-Lv121 CMV Stem/primary/others* Puromycin C-FLAG N/A
pReceiver-Lv102 CMV Stem/primary/others* Puromycin N-Flag N/A
pReceiver-Lv158 CMV Stem/primary/others* Neomycin C-FLAG N/A
pReceiver-Lv203 CMV Stem/primary/others* Puromycin C-Flag-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv216 CMV Stem/primary/others* Puromycin C-Flag-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv129 CMV Stem/primary/others* Puromycin C-Halotag Tev
pReceiver-Lv110 CMV Stem/primary/others* Puromycin N-Halotag Tev
pReceiver-Lv211 CMV Stem/primary/others* Hygromycin C-Halotag-IRES-hygromycin N/A
pReceiver-Lv128 CMV Stem/primary/others* Puromycin C-His N/A
pReceiver-Lv215 CMV Stem/primary/others* N/A IRES2-eGFP N/A
pReceiver-Lv205 CMV Stem/primary/others* Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv220 CMV Stem/primary/others* Hygromycin IRES2-Hygromycin N/A
pReceiver-Lv214 CMV Stem/primary/others* N/A IRES2-mCherry N/A
pReceiver-Lv213 CMV Stem/primary/others* Puromycin IRES2-mCherry-IRES-puromycin N/A
pReceiver-Lv111 CMV Stem/primary/others* Puromycin N-mCherry N/A
pReceiver-Lv130 CMV Stem/primary/others* Puromycin C-mCherry N/A
pReceiver-Lv155 CMV Stem/primary/others* Neomycin C-mCherry N/A
pReceiver-Lv107 CMV Stem/primary/others* Puromycin N-Myc N/A
pReceiver-Lv125 CMV Stem/primary/others* Puromycin C-Myc N/A
pReceiver-Lv126 CMV Stem/primary/others* Puromycin C-Myc-His N/A
pReceiver-Lv202 CMV Stem/primary/others* Puromycin C-Myc-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv204 CMV Stem/primary/others* Puromycin C-Myc-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv207 CMV Stem/primary/others* Hygromycin SV40-eGFP-IRES-hygromycin N/A
pReceiver-Lv201 CMV Stem/primary/others* Puromycin SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv217 CMV Stem/primary/others* Puromycin SV40-hLUC-IRES-puromycin N/A
pReceiver-Lv206 CMV Stem/primary/others* Puromycin SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv165 EF1a Stem/primary/others* N/A IRES2-eGFP N/A
pReceiver-Lv166 EF1a Stem/primary/others* N/A IRES2-mcherry N/A
pReceiver-Lv224 EF1a Stem/primary/others* Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv225 EF1a Stem/primary/others* Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv228 PGK Stem/primary/others* N/A IRES2-eGFP N/A
pReceiver-Lv229 PGK Stem/primary/others* N/A IRES2-mcherry N/A
pReceiver-Lv230 PGK Stem/primary/others* Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv231 PGK Stem/primary/others* Puromycin IRES2-eGFP-IRES-puromycin N/A
pLX304 CMV Stem/primary/others* Blasticidin C-V5 N/A

 

Download
 Plasmid Prep Protocol for Lentiviral clones

shRNA

Browse all shRNA lentiviral expression vectors and search for your clone of interest. Click to learn more about OmicsLink™ shRNA expression clones.

   

 

shRNA lentiviral expression vectors

 

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Vector Promoter Selection Marker Reporter Gene Viral type
psi-LVRH1GH H1 Hygromycin eGFP HIV
psi-LVRH1GP H1 Puromycin eGFP HIV
psi-LVRH1H H1 Hygromycin N/A HIV
psi-LVRH1MH H1 Hygromycin mCherry HIV
psi-LVRH1MP H1 Puromycin mCherry HIV
psi-LVRH1P H1 Puromycin N/A HIV
psi-LVRU6GH U6 Hygromycin eGFP HIV
psi-LVRU6GP U6 Puromycin eGFP HIV
psi-LVRU6H U6 Hygromycin N/A HIV
psi-LVRU6MH U6 Hygromycin mCherry HIV
psi-LVRU6MP U6 Puromycin mCherry HIV
psi-LVRU6P U6 Puromycin N/A HIV
psi-LVRInU6TGP inducible U6 Puromycin eGFP HIV

 

Download
 Plasmid Prep Protocol for Lentiviral clones

miRNA

Search for your clone of interest. Click to learn more about miExpress™ precursor miRNA expression clones and miArrest™ miRNA inhibitor clones.

   

 

microRNA precursor lentiviral vectors

 

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Vector Reporter gene Selection marker Promoter Viral type Type of miRNA
pEZX-MR02 mCherry Puromycin CMV HIV miRNA Precursor
pEZX-MR03 eGFP Puromycin CMV HIV miRNA Precursor

 

   

 

microRNA inhibitor lentiviral vectors

 

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Vector Reporter gene Selection marker Promoter Viral type Type of miRNA
pEZX-AM03 mCherry Hygromycin H1 HIV miRNA Inhibitors
pEZX-AM04 mCherry Hygromycin U6 HIV miRNA Inhibitors
pEZX-AM04p mCherry Puromycin U6 HIV miRNA Inhibitors

 

Download
 Plasmid Prep Protocol for Lentiviral clones

Promoter

Search for your clone of interest. Click to learn more about GLuc-ON promoter reporter clones.

 

Promoter reporter lentiviral vectors

 

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Vector Reporter gene Tracking gene Selection marker Viral type
pEZX-LvPG04 Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin HIV
pEZX-LvPG02 Gaussia luciferase (GLuc) N/A* Puromycin HIV
pEZX-LvPF02 eGFP N/A* Puromycin HIV
pEZX-LvPM02 mCherry N/A* Puromycin HIV
pEZX-LvPM03 tdTomato N/A* Puromycin HIV

 

Download
 Plasmid Prep Protocol for Lentiviral clones

CRISPR-Cas9

Search for your clone of interest. Click to learn more about Genome-CRISP™ CRISPR Products and Services.

 

sgRNA lentiviral vectors

 

Cas9 nuclease lentiviral expression clones

Vector Promoter Reporter gene Selection marker Viral type
CP-LvC9NU-01 CMV N/A Neomycin HIV
CP-LvC9NU-02 CMV eGFP Neomycin HIV

 

Download
 Plasmid Prep Protocol for Lentiviral clones

Publications

2014

 

2013

 

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