GeneCopoeia’s GLuc-ON™ NFκB transcriptional response element (TRE) clone enables analysis of the activity of NFκB-regulated signal transduction pathways. NFκB is a transcription factor with may gene targets, such as B-cell Activating Factor, T-cell secreted factor, and chemokines. NFκB plays critical roles in immune system function, and its misregulation can lead to inlammatory diseases and cancer.
The GLuc-ON™ NFκB TRE clone contains tandem repeats of NFκB upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The NFκB response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ NFκB TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ NFκB TRE by TNF-γ . 293T cells were transfected with a negative control clone or the GLuc-ON™ NFκB TRE clone. Transfected cells were incubated for 24 hours, followed by 18 hours of treatment with TNF-γ. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.