Lentivirus solutions

GeneCopoeia offers multiple lentiviral tools for over-expression or knockdown of your gene or microRNA of interest. With the option to buy the tools for producing lentivirus or buy pre-made lentivirus, GeneCopoeia collection of lentiviral tools seek to address your lentiviral research needs.

Lentiviral clones, kits and reagents

Express

• ORF cDNA: Express your protein with ready-to-express ORF clones in more than 40 lentiviral vectors with various fusion tags
• shRNA: Knockdown your target genes with shRNA clones available in lentiviral vectors with eGFP or mCherry fusion tags
• Precursor miRNA: Over-express miRNA with precursor miRNA clones available in lentiviral vectors with eGFP reporter gene
• miRNA inhibitors: Knockdown your miRNA of interest using lentiviral vector-based miRNA inhibitor clones

Package

• LentiPac packaging kits: Both FIV and HIV expression packaging kits
• Lentiviral packaging cell line: 293Ta packaging cells for easy and efficient transfection

Concentrate

• Lentivirus concentration solution: Recover 90% of lentivirus and increase titer by 10x-100x using this solution

Figure 1. Lentivirus generation

Ready made lentivirus

Pre-made Lentivirus

• Available for iPSC genes, luciferases, fluorescent genes, positive and negative control, many more
• Both Standard and purified options available

Special collection lentivirus service

• A low price and fast delivery service
• Offered for genome-wide human ORF cDNA and all known human, mouse and rat miRNA and miRNA inhibitors

Custom lentivirus service

• Available for any construct including ORF cDNA, shRNA, precursor miRNA and miRNA inhibitors
• Choice of 100s of expression vectors and fusion tags

Figure 2. H1299 cells (ATCC) in 24-well plates were transduced with the indicated amounts of Standard mCherry lentiviral particles, catalog number LP-MCHR-LV105 in the presence of 5 µg/ml of polybrene. The expression of eGFP was checked with a fluorescence microscope 72d hours post-transduction.

Publication highlights

Kraskiewicz, H. et al. (2011) Partial XBP1 knockdown does not affect viability of oligodendrocyte precursor cells exposed to new models of hypoxia and ischemia in vitro. Journal of Neuroscience Research 5 (89): 661-673 [rat XBP1 shRNA lentivirus

Nikkhah, M. et al. (2011) MCF10A and MDA-MB-231 human breast basal epithelial cell co-culture in silicon micro-arrays. Biomaterials 30 (32):7625-7632 [mCherry lentivirus]

Zheng, L. et al. (2011) Tyrosine 23 Phosphorylation-Dependent Cell-Surface Localization of Annexin A2 Is Required for Invasion and Metastases of Pancreatic Cancer. PLoS ONE 4 (6) [mouse ANXA2 shRNA Lentivirus]