Introduction
BlazeTaq™ SYBR
® Green qPCR Mix 2.0 is designed for highly sensitive and accurate quantification of gene expression and real-time PCR reactions. It contains a hot-start antibody-modified Taq DNA polymerase and optimized buffer system that avoids non-specific amplification of target DNA at lower temperatures, and enhances reaction mix performance. The reaction kit offers ready-to-use 5X Master Mix- just add the DNA template, primers and deionized water to start your fast and specific quantitative analysis.
The BlazeTaq™ SYBR
® Green qPCR Mix 2.0 can be used in combination with the
SureScript™ First-Strand cDNA Synthesis Kit as part of the two-step real-time RT PCR system.
Advantages
- Highly sensitive. Detects as little as 5 copies of DNA template.
- High specificity with minimal level of primer-dimer and non-specific product formation.
- High amplification efficiency over wide GC-content range.
- Faster heat activation than chemically-modified Taq: 30s vs 15 min.
- Less heat-damage to DNA samples than with chemically-modified Taq.
- More stable performance compared with chemically-modified Taq.
Product details
Product details
BlazeTaq™ SYBR
® Green qPCR mix 2.0 uses an antibody-modified Taq DNA polymerase to avoid polymerase activity prior to thermal cycling. Upon heating to 95 ºC for 30 seconds, the antibody dissociates and full activity of the Taq polymerase is restored. Also, the optimized buffer system allows high amplification efficiency and specificity, as well as enhanced sensitivity of real time PCR reactions over a wide range of templates.
Features
- 5X Master Mix for convenient use
- Hot-start polymerase and optimized buffer system to reduce non-specific reactions
- Available with or without ROX, allowing compatibility with all commonly used qPCR instruments
Performance
Wide Dynamic Range with High Sensitivity
DNA templates ranging from 5×10
7 (blue) to 5 copies (orange) were amplified. Linear regression of fluorescent signal on cycle numbers shows R
2 of 99.8% and amplification efficiency of 98.8%.
Performance comparison of BlazeTaq™ with competitor’s kit. Amplification of the B2M gene using serial diluted cDNA from HeLa cell line. Performance comparison of BlazeTaq™ (red) with BioRad SsoAdvanced™ Universal SYBR® Green Supermix (blue) shows stronger signals with similar amplification performance.
Performance comparison of BlazeTaq™ (red) with PowerUp™ SYBR Green Master Mix from Applied Biosystems (green), and GoTaq® qPCR Master Mix from Promega (blue) showed greater sensitivity, amplification efficiency and specificity.
Consistent detection of low copy number DNA templates
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Ct values shown for 50 and 5 copies of DNA templates, and no template control (NTC). For each template, the qPCR reactions were repeated 24 times.
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Amplification efficiencies range from 90% to 110% over a wide range of GC content.
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</p >DNA templates with different GC-content were serial-diluted and amplified.
