Lentifect™ Purified Lentivirus

For in vitro (cell culture) applications

Custom & Pre-made Particles

GeneCopoeia's Lentifect™ purified lentiviral particles for in vitro use are available either custom-made or pre-made. They are engineered for in vitro transduction of nearly all mammalian cells, including difficult-to-transfect cells. To ensure the highest possible titer and quality, Lentifect™ lentivirus is produced from standardized protocols, with stringent quality control processes, which include sequence verification of the insert and qRT-PCR based titer estimation. View transduction results of H1299 cells with Lentifect eGFP or mCherry lentivirus. 

For in vivo applications, GeneCopoeia also provides Lentifect™ Ultra-purified Lentivirus, which is subjected to further purification necessary for use in animal models.

Custom Lentifect™ Purified Lentivirus

Lentifect™ custom lentivirus production services are available for nearly any GeneCopoeia clone type, including:

  • ORF
  • CRISPR sgRNA
  • shRNA
  • miRNA precursors
  • miRNA inhibitors
  • Promoters
Search lentivirus

To order custom Lentifect™ purified lentiviral particles visit our search page.

Advantages

  • Convenience. Ready for transduction upon delivery.
  • High titer. ≥ 10^8 TU/ml, providing efficient transduction of virtually all mammalian cell types.
  • Versatility. Lentifect™ lentiviral particles can express nearly any GeneCopoeia clone type, including ORF (untagged or tagged), shRNA, sgRNA, miRNA and promoters.
  • Selection. Available in vectors with a wide variety of promoters, selection markers, and reporters.

 

Pre-made Lentifect™ Purified Lentivirus

Lentifect™ pre-made lentiviral titers typically range from 10^8 TU/mL and up and are available for immediate delivery for the following:

  • Positive and negative control lentivirus: eGFP, eYFP, eCFP, mCherry, PLUM, Firefly luciferase, Renilla luciferase, Gaussia luciferase
  • Other commonly used lentivirus: c-Myc, Klf4, Oct4, Sox2, Nanog, Lin28, CRE recombinase, FLP recombinase, SV40 large T antigen, HIV tat

Order positive and negative control lentiviral particles:

   
eGFP
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-mEGFP-LV105-025-C Monomeric eGFP Lentifect™ Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-mEGFP-LV105-100-C Monomeric eGFP Lentifect™ Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro
LPP-EGFP-LV105-025-C eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-EGFP-LV105-100-C eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro
LPP-EGFP-LV151-025-C eGFP Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Neo
LPP-EGFP-LV151-100-C eGFP Lentifect Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Neo
LPP-EGFP-LV152-025-C eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV152
LPP-EGFP-LV152-100-C eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV152
LPP-EGFP-LV156-025-C eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl EF1α Puro
LPP-EGFP-LV156-100-C eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl EF1α Puro

eYFP
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-EYFP-LV105-025-C eYFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-EYFP-LV105-100-C eYFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

eCFP
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-ECFP-LV105-025-C eCFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-ECFP-LV105-100-C eCFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

mCherry
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-MCHR-LV105-025-C mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-MCHR-LV105-100-C mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

PLUM
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-PLUM-LV105-025-C PLUM Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-PLUM-LV105-100-C PLUM Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

Firefly luciferase
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-HLUC-LV105-025-C Firefly Luciferase Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-HLUC-LV105-100-C Firefly Luciferase Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

Renilla luciferase
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-RLUC-LV105-025-C Renilla Luciferase Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-RLUC-LV105-100-C Renilla Luciferase Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

Gaussia luciferase
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-mGLUC-LV105-025-C Gaussia Luciferase Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-mGLUC-LV105-100-C Gaussia Luciferase Lentifect Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro

Firefly luciferase + eGFP/mCherry
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-HLUC-LV201-025-C Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV eGFP/Puro
LPP-HLUC-LV201-100-C Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV eGFP/Puro
LPP-HLUC-LV206-025-C Firefly Luciferase + mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV mCherry/Puro
LPP-HLUC-LV206-100-C Firefly Luciferase + mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV mCherry/Puro

Renilla luciferase + eGFP/mCherry
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-RLUC-LV201-025-C Renilla Luciferase + eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV eGFP/Puro
LPP-RLUC-LV201-100-C Renilla Luciferase + eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV eGFP/Puro
LPP-RLUC-LV206-025-C Renilla Luciferase + mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV mCherry/Puro
LPP-RLUC-LV206-100-C Renilla Luciferase + mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV mCherry/Puro

Firefly luciferase + IRES-eGFP
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-HLUC-LV215-025-C Firefly Luciferase + IRES-eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV215
LPP-HLUC-LV215-100-C Firefly Luciferase + IRES-eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV215

Renilla luciferase + IRES-eGFP
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-RLUC-LV215-025-C Renilla Luciferase + IRES-eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV215
LPP-RLUC-LV215-100-C Renilla Luciferase + IRES-eGFP Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV215

Firefly luciferase + IRES-mCherry
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-HLUC-LV214-025-C Firefly luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV214
LPP-HLUC-LV214-100-C Firefly luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV214

Renilla luciferase + IRES-mCherry
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-RLUC-LV214-025-C Renilla luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV214
LPP-RLUC-LV214-100-C Renilla luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV214

Promoter controls (GAPDH, SEAP)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-GAPDH-LVPF02-025-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPF02
LPP-GAPDH-LVPF02-100-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LVPF02
LPP-GAPDH-LVPM02-025-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPM02
LPP-GAPDH-LVPM02-100-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LVPM02
LPP-GAPDH-LVPM03-025-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPM03
LPP-GAPDH-LVPM03-100-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LVPM03
LPP-GAPDH-LVPG02-100-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LVPG02
LPP-GAPDH-LVPG02-025-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPG02
LPP-GAPDH-LVPG04-025-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPG04
LPP-GAPDH-LVPG04-100-C GAPDH Promoter Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LVPG04

Negative control
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-NEG-LV105-025-C Negative Control Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-NEG-LV105-100-C Negative Control Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro
LPP-NEG-LV151-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Neo
LPP-NEG-LV151-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Neo
LPP-NEG-LV152-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV152
LPP-NEG-LV152-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV152
LPP-NEG-LV156-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-NEG-LV156-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV Puro
LPP-NEG-LV201-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV eGFP/Puro
LPP-NEG-LV201-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV eGFP/Puro
LPP-NEG-LV206-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV mCherry/Puro
LPP-NEG-LV206-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV mCherry/Puro
LPP-NEG-LV214-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV214
LPP-NEG-LV214-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV214
LPP-NEG-LV215-025-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LV215
LPP-NEG-LV215-100-C Negative Control Lentifect Purified Lentiviral Particles
(25 µl x 4 vials)
Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LV215

miRNA scrambled controls
Buy Catalog# Product Description Vector
LPP-CmiR0001-MR02-025-C miRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl pEZX-MR02
LPP-CmiR0001-MR02-100-C miRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl pEZX-MR02
LPP-CmiR0001-MR03-025-C miRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl pEZX-MR03
LPP-CmiR0001-MR03-100-C miRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl pEZX-MR03

miRNA inhibitor scrambled controls
Buy Catalog# Product Description Vector
LPP-CmiR-AN0001-AM03-025-C miRNA Inhibitor Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl pEZX-AM03
LPP-CmiR-AN0001-AM03-100-C miRNA Inhibitor Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl pEZX-AM03
LPP-CmiR-AN0001-AM04-025-C miRNA Inhibitor Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl pEZX-AM04
LPP-CmiR-AN0001-AM04-100-C miRNA Inhibitor Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl pEZX-AM04
LPP-CmiR-AN0001-AM04p-025-C miRNA Inhibitor Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl pEZX-AM04p
LPP-CmiR-AN0001-AM04p-100-C miRNA Inhibitor Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl pEZX-AM04p

shRNA scrambled controls
Buy Catalog# Product Description Vector
LPP-CSHCTR001-LVRH1GH-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRH1GH
LPP-CSHCTR001-LVRH1GH-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRH1GH
LPP-CSHCTR001-LVRH1GP-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRH1GP
LPP-CSHCTR001-LVRH1GP-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRH1GP
LPP-CSHCTR001-LVRH1H-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRH1H
LPP-CSHCTR001-LVRH1H-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRH1H
LPP-CSHCTR001-LVRH1MH-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRH1MH
LPP-CSHCTR001-LVRH1MH-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRH1MH
LPP-CSHCTR001-LVRH1MP-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRH1MP
LPP-CSHCTR001-LVRH1MP-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRH1MP
LPP-CSHCTR001-LVRH1P-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRH1P
LPP-CSHCTR001-LVRH1P-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRH1P
LPP-CSHCTR001-LVRU6GH-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRU6GH
LPP-CSHCTR001-LVRU6GH-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRU6GH
LPP-CSHCTR001-LVRU6GP-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRU6GP
LPP-CSHCTR001-LVRU6GP-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRU6GP
LPP-CSHCTR001-LVRU6H-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRU6H
LPP-CSHCTR001-LVRU6H-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRU6H
LPP-CSHCTR001-LVRU6MH-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRU6MH
LPP-CSHCTR001-LVRU6MH-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRU6MH
LPP-CSHCTR001-LVRU6MP-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRU6MP
LPP-CSHCTR001-LVRU6MP-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRU6MP
LPP-CSHCTR001-LVRU6P-025-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,25µl psi-LVRU6P
LPP-CSHCTR001-LVRU6P-100-C shRNA Scrambled Control Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml control lentiviral particles ready for transduction,100µl psi-LVRU6P

sgRNA scrambled controls
Buy Catalog# Product Description Vector
LPPCCPCTR01L03-025 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 25 µl (U6/T7/mCherry/Puro)
LPPCCPCTR01L03-100 Purified Lentifect™ Lentiviral Particles of scrambled sgRNA control CCPCTR01-LvSG03 >1×108 TU/ml sgRNA control lentiviral particles ready for transduction, 100 µl (U6/T7/mCherry/Puro)

Promoter scrambled controls
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-NEG-LVPF02-025-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPF02
LPP-NEG-LVPF02-100-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 100 µl CMV LVPF02
LPP-NEG-LVPM02-025-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPM02
LPP-NEG-LVPM02-100-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPM02
LPP-NEG-LVPM03-025-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPM03
LPP-NEG-LVPM03-100-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPM03
LPP-NEG-LVPG02-025-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPG02
LPP-NEG-LVPG02-100-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPG02
LPP-NEG-LVPG04-025-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPG04
LPP-NEG-LVPG04-100-C Promoter Scrambled Control Lentiviral Particles Purified > 1x108 TU/ml control lentiviral particles ready for transduction, 25 µl CMV LVPG04

Order other commonly-used lentiviral particles:

c-Myc (Human)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-CMYC-LV105-025-C c-Myc Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified >1x108 TU/ml c-Myc lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-CMYC-LV105-100-C c-Myc Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified >1x108 TU/ml c-Myc lentiviral particles ready for transduction, 100 µl CMV Puro

Klf4 (Human)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-KLF4-LV105-025-C Klf4 Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified >1x107 TU/ml Klf4 lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-KLF4-LV105-100-C Klf4 Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified >1x107 TU/ml Klf4 lentiviral particles ready for transduction, 100 µl CMV Puro

Oct4 (Human)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-OCT4-LV105-025-C Oct4 Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified >1x107 TU/ml Oct4 lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-OCT4-LV105-100-C Oct4 Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified >1x107 TU/ml Oct4 lentiviral particles ready for transduction, 100 µl CMV Puro

Sox2 (Human)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-SOX2-LV105-025-C Sox2 Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified >1x107 TU/ml Sox2 lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-SOX2-LV105-100-C Sox2 Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified >1x107 TU/ml Sox2 lentiviral particles ready for transduction, 100 µl CMV Puro

Nanog (Human)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-Z8289-LV105-025-C Nanog Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified >1x108 TU/ml Nanog lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-Z8289-LV105-100-C Nanog Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified >1x108 TU/ml Nanog lentiviral particles ready for transduction, 100 µl CMV Puro

LIN28A (Human)
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-LIN28A-LV105-025-C Lin28 Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified >1x108 TU/ml Lin28 lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-LIN28A-LV105-100-C Lin28 Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified >1x108 TU/ml Lin28 lentiviral particles ready for transduction, 100 µl CMV Puro

CRE recombinase
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-CRE-LV201-025-C CRE recombinase Purified Lentifect™ Lentiviral Particles
(25 µl x 1 vial)
Purified >1x108 TU/ml CRE recombinase lentiviral particles ready for transduction, 25 µl CMV eGFP
Puromycin
LPP-CRE-LV201-100-C CRE recombinase Purified Lentifect™ Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml CRE recombinase lentiviral particles ready for transduction, 100 µl CMV eGFP
Puromycin

FLP recombinase
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-FLP-LV201-025-C FLP recombinase Purified Lentifect™ Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml FLP recombinase lentiviral particles ready for transduction, 25 µl CMV eGFP
Puromycin
LPP-FLP-LV201-100-C FLP recombinase Purified Lentifect™ Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml FLP recombinase lentiviral particles ready for transduction, 100 µl CMV eGFP
Puromycin

SV40 large T antigen
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-SV40T-LV105-025-C SV40 large T antigen Purified Lentifect™ Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml SV40 large T antigen lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-SV40T-LV105-100-C SV40 large T antigen Purified Lentifect™ Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml SV40 large T antigen lentiviral particles ready for transduction, 100 µl CMV Puro

HIV tat
Buy Catalog# Product Type Description Promoter Reporter/ Marker
LPP-TAT-LV105-025-C HIV TAT Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
Purified > 1x108 TU/ml HIV tat lentiviral particles ready for transduction, 25 µl CMV Puro
LPP-TAT-LV105-100-C HIV TAT Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
Purified > 1x108 TU/ml HIV tat lentiviral particles ready for transduction, 100 µl CMV Puro

Cell structure lentivirus
Buy Catalog# Product Description Vector
LP501 Actin-RFP fusion Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
> 1x108 TU/ml Actin-RFP fusion lentiviral particles ready for transduction, 25 µl pEZ-Lv105
LP502 Actin-RFP fusion Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
> 1x108 TU/ml Actin-RFP fusion lentiviral particles ready for transduction, 100 µl pEZ-Lv105
LP506 Tubulin-RFP fusion Lentifect™ Purified Lentiviral Particles
(25 µl x 1 vial)
> 1x107 TU/ml Tubulin-RFP fusion lentiviral particles ready for transduction, 25 µl pEZ-Lv105
LP507 Tubulin-RFP fusion Lentifect™ Purified Lentiviral Particles
(25 µl x 4 vial)
> 1x107 TU/ml Tubulin-RFP fusion lentiviral particles ready for transduction, 100 µl pEZ-Lv105

Buy Catalog Product Name Description Price
LPP-CP-LvC9NU-01-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) >1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (CMV/Neomycin) inquire
LPP-CP-LvC9NU-02-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (CMV/eGFP/Neomycin) inquire
LPP-CP-LvC9NU-08-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/Puromycin) inquire
LPP-CP-LvC9NU-09-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/eGFP/Neomycin) inquire
LPP-CP-LvC9NU-10-100-C Cas9 Nuclease Purified Lentifect™ Lentiviral Particles (100 µl x 1 vial) > 1x107 TU/ml Cas9 nuclease lentiviral particles ready for transduction,100 µl (EF1α/eGFP/Hygromycin) inquire

 

Download datasheets for positive and negative control lentiviral particles

Background and Data

Lentiviral vectors are potent vehicles for delivery of genes into a wide range of cell types including difficult-to-transfect cells. Lentiviral particles transduce cells and integrate into the host genome in dividing and post-mitotic cells resulting in long-term expression of the transgene in vitro. Producing, concentrating and titrating lentiviral particles, however, is time consuming and requires some experience to achieve high titers and consistent results. GeneCopoeia's Lentifect™ purified lentiviral particles save you time and effort, and provide high-level expression of genetic elements of interest (Figure 1).

Transduction of H1299 cells with GeneCopoeia's Lentifect™ lentiviral particles expressing eGFP.

Figure 1. H1299 cells in 24-well plates were transduced with the indicated amounts of eGFP purified particles, (catalog number LPP-EGFP-LV105) in the presence of 5 µg/ml of polybrene. The expression of eGFP was visualized with a fluorescence microscope 72 hours post-transduction.

FAQs

Frequently Asked Questions: Lentivirus

Answer: The best way is to search for what you need on our lentiviral search page. You simply need to indicate the clone type (ORF, promoter, etc.), the gene information (gene symbol, aliases, description, nucleotide accession, Entrez gene ID, catalog or product ID), the vector type, and the delivery volume. You can also request a custom quote by contacting us.
Answer: 50 μl, 100 μl, 200 μl, and 400 μl, at titers of 10^7-10^9 TU/mL.
Answer: Lentiviruses comprise a subtype of retroviruses. Lentiviruses can stably integrate into the host genome in dividing, non-dividing and post-mitotic mammalian cells, while retroviruses are less active in this scenario. Adenoviruses can also transduce non-dividing cells, but can’t stably integrate into the host cell’s genome. Adenoviruses also take much more time to design and prepare. In addition, lentiviruses are much less immunogenic than the retroviruses and adenoviruses, making lentivirus more suitable for use in most types of cells and animal models.
Answer: One of the key factors of a successful transduction is the cell type. For example, transduction efficiency is much higher in actively dividing cells than in non-dividing cells. In addition, transduction of cells works better at lower MOI (multiplicity of infection) than at higher MOI. MOI is the ratio of the number of lentivirus particles to the number of cells. For some cell types, the higher the MOI , the larger the volume and higher the titer of lentivirus is required in order for the experiment to succeed. You can adjust the cell number and add the appropriate amount of lentivirus according to what has been reported in the scientific literature. If there is no adequate information in the scientific literature, we recommend performing a preliminary experiment using gradient dilutions of lentivirus, such as 0.1 μl, 0.3 μl, 0.5 μl, 0.7 μl, 0.9 μl for GeneCopoeia purified particles. Another important consideration for getting good transduction efficiency is the cell status. Transduction efficiency varies greatly between healthy cells and unhealthy cells. Therefore, it is essential to keep the cells as healthy as possible. For some cells with high MOI, you could also include additives such as polybrene to enhance the transduction efficiency. However, the overall health of the cells itself is always the most essential element.
Answer: Before lentivirus production starts, you need to first prepare the plasmid DNA using a well-established purification method. Make sure the plasmid you prepare is of the highest possible quality. You can measure its purity by the absorption ratio of 260 nm to 280 nm. You should also check the integrity of the plasmid by agarose gel electrophoresis. GeneCopoeia can provide your clones in transfection-ready format. In rare cases, toxic genes and large fragment inserts lead to low titers. Next, ensure your lentiviral packaging cell line is well maintained and passaged regularly, and make sure the culture is free from contamination of bacteria, fungi, and/or mycoplasma. Further, use an optimized lentivirus packaging system and reagent. We recommend the GeneCopoeia Lenti-Pac™ HIV-Based Lentiviral Packaging system, which is optimized for production of high viral titer, together with the GeneCopoeia Lenti-Pac™ HIV Expression Packaging Kit. We also recommend using GeneCopoeia 293Ta lentiviral packaging cells (Cat No. CLv-PK-01). These cells are guaranteed to provide higher transfection efficiency and lower cell toxicity.

Answer: There are two main GOI-related factors that can affect the lentiviral titer. These are:

Toxicity. Some genes expressed from lentiviruses are toxic to cells and so will sharply reduce the titer.

Insert length. Larger inserts also tend to reduce viral titer.

The issue of insert length must be considered along with the backbone vector size, because the viral titer is influenced by the total length of the plasmid. Our recommendations for insert size for lentivirus packaging are shown below. Note that these are only general guidelines:

For Lv200 series vectors (such as Lv201 and Lv206), the insert should be less than 4 Kb.

For most Lv100 series vectors, except for those with eGFP fusions, the insert should be less than 5kb.

For other stripped-down vectors (without reporter or selection gene), the insert plus HIV related parts in the packing vector should be less than 10 Kb.

Answer: In general, a lentivirus packaging system contains a gene transfer vector plasmid and a packaging plasmid. The second and third generation lentivirus packaging systems are both designed to separate the essential genes of the transfer vector, envelope and packaging components onto different plasmids, thereby reducing the risk of recombination. When some lentiviral structural proteins must be expressed along with the gene of interest in the second generation, the vector of the third generation is revamped to make it self-inactivating and tat-independent. The GeneCopoeia™ HIV-Based Lentiviral Expression System is a modified version of the third generation self-inactivating (SIN) lentiviral vector system, which incorporates enhanced biosafety features and is optimized for production of high viral titers.
Answer: The titer of the GeneCopoeia lentivirus products in each lot are determined by qRT-PCR, which shows the physical number of viral genomic RNA molecules. The numerical relationship between titer (physical copy number) and transduction unit (TU or IFU) can be basically summarized using the following formula: TU= Titer (physical copy number)/100. The titer may vary among different cell types. In general, we recommended conducting a preliminary experiment before your formal study to ensure the viability of the lentivirus stock and to test the amount needed to transduce the cell type of interest. For more information regarding titer estimation by transduction, you may download a copy of the user manual.
Answer: It is safe to use GeneCopoeia lentivirus. The GeneCopoeia HIV-Based Lentiviral Expression System meets Biosafety Level 2 (BSL-2) requirements based on the criteria published by the Centers for Disease Control and Prevention (CDC). This system is a modified version of the third generation of the self-inactivating (SIN) lentiviral vector system, which incorporates enhanced biosafety features. The lentiviral transfer vector is responsible for transduction and stable integration into the genome of the host cell, but lacks the elements essential for transcription and packaging lentiviral particles by itself. Thus, it is self-inactivated, meaning that no unwanted viral replication and production will happen after the first transfection. Nevertheless, the guidelines for working with BSL-2 safety category materials must be adhered to. For more information regarding BSL-2, please visit the CDC website.
Answer: GeneCopoeia offers a vast number of clones in both HIV-based and FIV-based lentiviral systems, including more than 40,000 human and mouse ORF expression clones, small hairpin RNAi (shRNA) against genome-wide target genes from human, mouse, rat and other animals, miRNA inhibitor clones for all known human, mouse and rat miRNAs, promoter reporter clones for more than 20,000 human and 18,000 mouse promoters, and CRISPR sgRNA clones for human and mouse. This system provides high expression levels and high efficiency of gene delivery to virtually all mammalian cell types. The lentiviral expression construct was validated by full-length sequencing, restriction enzyme digestion, and PCR-size validation using gene-specific and vector-specific primers. Together with the GeneCopoeia EndoFectin Lenti Reagent (Cat No. EFL1001-01), TiterBoost™ reagent, 293Ta lentiviral packaging cells (Cat No. CLv-PK-01) and MycoGuard™mycoplasma detection kit, GeneCopoeia lentiviral products provide high viral titer and are confirmed free of bacteria, fungi and common mycoplasma contamination.
Answer: Lentivirus has some level of toxicity to cells. It may cause damage to your cell of interest with either superfluous amounts of lentivirus, or if the infection were allowed to go on for too long a period of time. In these cases, you can adjust the multiplicity of infection (MOI) to a lower range. We recommend replacing the old culture medium with fresh complete medium 4-8 hours post transduction (no later than 12 hours post transduction).
Answer: Lentiviruses can stably integrate into the host cell’s genome and obtain a consistent level of expression. With a selectable marker in the lentiviral gene transfer vector plasmid, it is easy to generate a stable cell line using drug selection. You can use qRT-PCR, western blot or other detection methods to estimate the expression level of your gene.

*Promotions are valid in US and Canada only. Discount offer is valid for 200ul and 400ul lentivirus only. Discount offer is not valid for custom services. Promotion ends August 31st, 2017. If customer cannot accept gift card, a $25 discount can be applied to the order instead. $25 gift card offer is not valid for gene synthesis products. Limit one gift card per order. Gift card is valid in US and Canada only. Not valid for previous purchases. Health care professionals and employees or those working on behalf of federal, state, or local government entities may not participate in this promotion. By accepting the gift, customers represent that they are not prohibited by employment, contract, law, regulation, or agency/institutional policy from receiving the gift from GeneCopoeia. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

Guaranteed titer > 10^8 TU/ml is valid for all lentivirus orders placed on or after 9/1/2016. Not valid for previous purchases.