For Stem Cells and Primary Culture Cells and Other Mammalian Cells
Multiple sets of OmicsLink™ lentiviral vector based ORF cDNA clones for more than 20,000 human and 15,000 mouse genes were generated from high quality cDNA libraries, individually quality checked, available now, and ready for transfection and generation of viral particles.
Advantages of OmicsLink™ Lentiviral ORF Expression Clones
High Efficiency of Gene Delivery to Virtually all Cell Types and Whole Model Organisms
The Lentiviral system is very effective at delivering genetic material to whole model organisms and almost all mammalian cells, including non-dividing cells, cells that are not active or growing, and difficult to transfect cells such as neurons, primary cells and stem cells. The efficiency of lentiviral transduction is close to 100%, which makes the lentiviral vector ideal as an expression vector system.
High Expression Levels of Delivered Genes
20,000 human genes have been cloned into three types (N-,C-terminal tagged or non-tagged) of Lentiviral expression vectors. They contain the sequence features and elements allowing efficient packaging, transduction and stable integration into genomic DNA of target cells, and enabling high levels of expression and detection of any one of our 16,000 human genes in those cells.
Self-inactivation and No Unwanted Viral Replication
OmicsLink™ ORF lentiviral expression clones are self-inactivating. Transduced cells cannot produce additional viral particles since these cells do not contain the genes needed to produce the viral capsid. Furthermore, upon integration into target cell's genome, the 5' LTR promoter is inactivated, which prevents replication of the viral sequence.
OmicsLink™ ORF Expression Clones are available in the following vector types:
ORF Expression Clones are also available in 80 or so other expression vector systems with different promoters and various N- or C-terminal tags: His, GST, MBP, eGFP, eYFP, AviTag™, HA, Flag, HaloTag®, SUMO, and more.