Advantages of lentiviral ORF expression clones
High efficiency of gene delivery to virtually all cell types and whole model organisms
A lentiviral vector system is very effective at delivering genetic material to whole model organisms and almost all mammalian cells, including non-dividing, inactive or growing, and difficult-to-transfect cells including neuron, primary and stem cells. The efficiency of lentiviral transduction is close to 100%, making it ideal as an expression vector system.
High expression levels of delivered genes
Lentiviral expression vectors with N-or-C terminal fusion tag or un-tagged consist of sequence features and elements allowing efficient packaging, transduction and stable integration into genomic DNA of target cells. Thus, enabling high levels of expression and detection of ORF insert in the target cells.
Self-inactivation and no unwanted viral replication
OmicsLink ORF cDNA lentiviral expression clones are self-inactivating. Transduced cells cannot produce additional viral particles since these cells do not contain the genes needed to produce the viral capsid. Furthermore, upon integration into target cell's genome, the 5' LTR promoter is inactivated, which prevents replication of the viral sequences. |
