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GeneCopoeia – ORF cDNA, shRNA, miRNA
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Lentiviral vector-based ORF cDNA, shRNA and microRNA clones

GeneCopoeia is the original source of ORF cDNA clones and also provides the most comprehensive collection of lentiviral vectors with the largest choice of fusion tags including IRES-eGFP, Avitag®, HaloTag®.

With stringent quality control followed at every step during production, these constructs are fully sequenced and ready-for-expression.

Combined with the ORF cDNA constructs, GeneCopoeia also provide constructs for shRNA, precursor miRNA, and miRNA inhibitors in lentiviral vector backbone.

Optimized packaging plasmids, cell line and lentivirus concentration solution are also offered as a part of the comprehensive suite of lentiviral tools.

 

Advantages of lentiviral ORF expression clones

High efficiency of gene delivery to virtually all cell types and whole model organisms

A lentiviral vector system is very effective at delivering genetic material to whole model organisms and almost all mammalian cells, including non-dividing, inactive or growing, and difficult-to-transfect cells including neuron, primary and stem cells. The efficiency of lentiviral transduction is close to 100%, making it ideal as an expression vector system.

High expression levels of delivered genes

Lentiviral expression vectors with N-or-C terminal fusion tag or un-tagged consist of sequence features and elements allowing efficient packaging, transduction and stable integration into genomic DNA of target cells. Thus, enabling high levels of expression and detection of ORF insert in the target cells.

Self-inactivation and no unwanted viral replication

OmicsLink ORF cDNA lentiviral expression clones are self-inactivating. Transduced cells cannot produce additional viral particles since these cells do not contain the genes needed to produce the viral capsid. Furthermore, upon integration into target cell's genome, the 5' LTR promoter is inactivated, which prevents replication of the viral sequences.

  Flowchart for producing lentiviral vectors to lentivirus

ORF cDNA lentiviral vectors

Vector Promoter Host Cell Selecting Marker Tag Protease Site
pReceiver-Lv01 CMV Stem/primary/others* No N/A N/A
pReceiver-Lv105 CMV Stem/primary/others* Puromycin N/A N/A
pReceiver-Lv151 CMV Stem/primary/others* Neomycin N/A N/A
pReceiver-Lv152 CMV Stem/primary/others* Hygromycin N/A N/A
pReceiver-Lv81 CMV Stem/primary/others* N/A IRES2-eGFP N/A
pReceiver-Lv76 PGK Stem/primary/others* N/A IRES2-eGFP N/A
pReceiver-Lv78 CMV Stem/primary/others* N/A IRES2-eCFP N/A
pReceiver-Lv79 CMV Stem/primary/others* N/A IRES2-eYFP N/A
pReceiver-Lv80 CMV Stem/primary/others* N/A IRES2-mcherry N/A
pReceiver-Lv36 CMV Stem/primary/others* No IRES-luciferase N/A
pReceiver-Lv40 CMV Stem/primary/others* Neomycin IRES-Neomycin N/A
pReceiver-Lv21 CMV Stem/primary/others* Neomycin N/A N/A
pReceiver-Lv02 CMV Stem/primary/others* No C-3xHA N/A
pReceiver-Lv52 CMV Stem/primary/others* N/A C-3xHA+IRES2-eGFP N/A
pReceiver-Lv06 CMV Stem/primary/others* Neomycin C-3xHA N/A
pReceiver-Lv23 CMV Stem/primary/others* Neomycin N-Flag N/A
pReceiver-Lv03 CMV Stem/primary/others* No C-Flag N/A
pReceiver-Lv53 CMV Stem/primary/others* N/A C-Flag+IRES2-eGFP N/A
pReceiver-Lv07 CMV Stem/primary/others* Neomycin C-Flag N/A
pReceiver-Lv19 CMV Stem/primary/others* Neomycin N-eGFP N/A
pReceiver-Lv04 CMV Stem/primary/others* No C-eGFP N/A
pReceiver-Lv08 CMV Stem/primary/others* Neomycin C-eGFP N/A
pReceiver-Lv20 CMV Stem/primary/others* Neomycin N-eYFP N/A
pReceiver-Lv05 CMV Stem/primary/others* No C-eYFP N/A
pReceiver-Lv09 CMV Stem/primary/others* Neomycin C-eYFP N/A
pReceiver-Lv34 CMV Stem/primary/others* Neomycin N-eCFP N/A
pReceiver-Lv61 CMV Stem/primary/others* No C-eCFP N/A
pReceiver-Lv62 CMV Stem/primary/others* Neomycin C-eCFP N/A
pReceiver-Lv68 CMV Stem/primary/others* N/A C-Avi+IRES-Biotin ligase N/A
pReceiver-Lv35 CMV Stem/primary/others* No N-Avi+IRES-Biotin ligase N/A
pReceiver-Lv26 CMV Stem/primary/others* Neomycin N-Avi N/A
pReceiver-Lv10 CMV Stem/primary/others* Neomycin C-Avi N/A
pReceiver-Lv25 CMV Stem/primary/others* Neomycin N-Myc N/A
pReceiver-Lv17 CMV Stem/primary/others* Neomycin C-Myc N/A
pReceiver-Lv18 CMV Stem/primary/others* Neomycin C-Myc-His N/A
pReceiver-Lv70 CMV Stem/primary/others* N/A C-Myc+IRES2-eGFP N/A
pReceiver-Lv47 CMV Stem/primary/others* Neomycin C-Myc+IRES-Neomycin N/A
pReceiver-Lv77 PGK Stem/primary/others* N/A C-Myc+IRES2-eGFP N/A
pReceiver-Lv75 CMV Stem/primary/others* N/A C-Myc+IRES2-mcherry N/A
pReceiver-Lv48 CMV Stem/primary/others* N/A C-Myc+IRES2-eYFP N/A
pReceiver-Lv45 CMV Stem/primary/others* N/A C-Myc+IRES2-eCFP N/A
pReceiver-Lv46 CMV Stem/primary/others* N/A C-Myc+IRES-luciferase N/A
pReceiver-Lv64 CMV Stem/primary/others* Neomycin N-HaloTag Tev protease
pReceiver-Lv65 CMV Stem/primary/others* Neomycin C-HaloTag Tev protease
pReceiver-Lv71 CMV Stem/primary/others* Puromycin N-mCherry N/A
pReceiver-Lv72 CMV Stem/primary/others* Neomycin C-mCherry N/A
pReceiver-Lv73 CMV Stem/primary/others* N/A C-His+IRES-eGFP N/A
pReceiver-Lv66 CMV Stem/primary/others* Hygromycin N/A N/A
pReceiver-Lv41 EF1a Stem/primary/others* Neomycin N/A N/A
pReceiver-Lv67 CMV Stem/primary/others* Puromycin N/A N/A

* Lentiviral vectors can also be used for virtually any mammalian cell type

shRNA lentiviral expression vectors

search clone
Vector Promoter Selection Marker Reporter Gene Viral type
psiLv-H1 H1 Puromycin eGFP Lenti
psiLv-U6 U6 Puromycin eGFP Lenti
psiLv-mH1 H1 Puromycin mCherry Lenti
psiLv-mU6 U6 Puromycin mCherry Lenti

microRNA and microRNA inhibitor lentiviral vectors

search clone
Vector Reporter gene Selection marker Promoter Viral type Type of miRNA
pEZX-AM03 mCherry Hygromycin H1 HIV miRNA Inhibitors
pEZX-AM04 mCherry Hygromycin U6 HIV miRNA Inhibitors
pEZX-MR01 eGFP Neomycin FIV FIV miRNA Precursor


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