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RNAzol® RT RNA Isolation Reagent

Introduction

RNAzol®RT is the most effective reagent for isolation of total RNA and small RNA from samples of various origins, including human, animal, plant, bacterial and viral origin.

RNAzol®RT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA.  It isolates mRNA and microRNA in separate fractions. Alternatively, total RNA containing all classes of RNA in a single fraction can be isolated. In addition, the patented reagent allows for the sequential isolation of RNA and DNA.

Advantages

Higher yield and quality

  • Isolate pure and undegraded RNA that is ready for RT-PCR without DNase treatment
  • Provides higher yield and quality of isolated RNA than previous reagents

Easy isolation of mRNA, microRNA or total RNA

  • Isolate mRNA and microRNA in separate fractions
  • Isolate total RNA in one fraction

Fast and convenient

  • The isolation procedure can be completed in less than one hour
  • No chloroform-induced phase separation is necessary
  • Stable at room temperature for both storage and experimental procedure

 

Product

Buy Catalog# Product Size Price
QP020 RNAzol® RT RNA Isolation Reagent 50ml inquire

 

 

References

  1. Chomczynski P, Reagents and methods for isolation of purified RNA, US Patent# 7,794,932, International Patents Pending.
  2. Chomczynski P, and Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol- chloroform extraction. Anal. Biochem. 162, 156 – 159.
  3. Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532 – 537.
  4. Wilfinger W, Mackey K and Chomczynski P (1997) Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 22, 474 – 481.

 

Related Products

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qPCR Arrays

RNAzol®RT is the registered trademark of Molecular Research Center, Inc. US Patent 7794932, Chinese Patent 200580017853.5. International Patent application is pending.  

Publications

2017

2016