RNAzol®RT is the most effective reagent for isolation of total RNA and small RNA from samples of various origins, including human, animal, plant, bacterial and viral origin.
RNAzol®RT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. It isolates mRNA and microRNA in separate fractions. Alternatively, total RNA containing all classes of RNA in a single fraction can be isolated. In addition, the patented reagent allows for the sequential isolation of RNA and DNA.
Higher yield and quality
- Isolate pure and undegraded RNA that is ready for RT-PCR without DNase treatment
- Provides higher yield and quality of isolated RNA than previous reagents
Easy isolation of mRNA, microRNA or total RNA
- Isolate mRNA and microRNA in separate fractions
- Isolate total RNA in one fraction
Fast and convenient
- The isolation procedure can be completed in less than one hour
- No chloroform-induced phase separation is necessary
- Stable at room temperature for both storage and experimental procedure
- Chomczynski P, Reagents and methods for isolation of purified RNA, US Patent# 7,794,932, International Patents Pending.
- Chomczynski P, and Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol- chloroform extraction. Anal. Biochem. 162, 156 – 159.
- Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532 – 537.
- Wilfinger W, Mackey K and Chomczynski P (1997) Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 22, 474 – 481.
qPCR kits and primers
- All-in-One™ miRNAqRT-PCR Reagent Kits and Validated Primers
- All-in-One™ qPCR Mix and Validated Primers
- All-in-One™ First-Strand cDNA Synthesis Kit
- miProfile™ Cancer miRNAqPCR Arrays
- miProfile™ Disease and Focus-Group miRNAqPCR Arrays
- miProfile™ miRNAqPCR Arrays
RNAzol®RT is the registered trademark of Molecular Research Center, Inc. US Patent 7794932, Chinese Patent 200580017853.5. International Patent application is pending.
- Wang, S., et al. (2016). Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. Acta Pharmacologica Sinica doi: 10.1038/aps.2016.118 [RNAzol®RT reagent]