A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from most any source of RNA
- Efficient and easy
- Cost efficient
The All-in-One™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.
The kit uses Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
All-in-One First-Strand Synthesis Kit in action
An example showing assessment of reverse transcription efficiency using the All-in-One First Strand Synthesis Kit is shown below. The amplification results of different genes or gene regions using the oligo(dT) synthesized cDNA prepared from the All-in-One First-Strand cDNA Kit.
Figure 1. Synthesis Kit by oligo(dT) primer. The synthesized cDNA from human placenta was then used to amplify different gene regions by quantitative PCR using the All-in-One qPCR Mix (GeneCopoeia Catalog No. AOPR-0200). The positive amplification result of MACF1 indicates that up to 13 kb RNA sequence was reversed transcribed.
|All-in-One™ qPCR Mix||SYBR Green-based real-time
quantitative PCR Mix
|High-fidelity,hot-start DNA polymerase ,ROX reference dye,optimized reaction buffer and dNTPs|
|All-in-One™qPCR Primers||Human, mouse and rat primers||Validated, gene-specific primers ensure specificity and sensitivity|
|RNAzol®RT RNA Isolation Reagent||Isolation of total RNA and small RNA||Easy isolation of mRNA, miRNA and total RNA. Ready for RT-PCR without DNase treatment|
|miProfile™ miRNA qPCR Array||User specified, ready-to-use
primer arrays in 96-well-plate
|Reliable tools ideal for analyzing the expression of a focused panel of miRNAs such as pathways, diseases or customized gene panels|
|ExProfile™ Gene qPCR Arrays||User specified, ready-to-use primer arrays in 96-well-plate||For expression profiling of pre-defined or customized sets of genes in various tissues or cells|
|All-in-One™ miRNA qRT-PCR Detection Kits||SYBR® Green-based||Accurately quantify miRNA expression|
|All-in-One™ miRNA qPCR Primers||Human, mouse and rat primers||Validated for robust, reproducible and reliable quantitation of miRNA activity|
|OmicsLink™ Expression-Ready ORF cDNA Clones||20,000 human
|Perform gain-of-function studies with expression-ready clones|
|Optimized for specific cell types||Transfect efficiently and with low toxicity|