All-in-One™ First-Strand cDNA Synthesis Kit — robust cDNA from most any total or polyA RNA source
A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from most any source of RNA
Efficient and easy
Reliable
Cost efficient
The All-in-One™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.
The kit uses Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
All-in-One First-Strand Synthesis Kit in action
An example showing assessment of reverse transcription efficiency using the All-in-One First Strand Synthesis Kit is shown below. The amplification results of different genes or gene regions using the oligo(dT) synthesized cDNA prepared from the All-in-One First-Strand cDNA Kit.
Figure 1.Synthesis Kit by oligo(dT) primer. The synthesized cDNA from human placenta was then used to amplify different gene regions by quantitative PCR using the All-in-One qPCR Mix (GeneCopoeia Catalog No. AOPR-0200). The positive amplification result of MACF1 indicates that up to 13 kb RNA sequence was reversed transcribed.
