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miProfile™ miRNA qPCR Arrays

Introduction

For high-throughput profiling of miRNA expression

 

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended  and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

Key advantages

Validated miRNA primers

  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates

Genome-wide coverage, pre-arranged groups, or customized groups

  • Largest genome-wide miRNA coverage
  • Cancer-related groups
  • Customized miRNA arrays for focused study

 

Figure 1. miRNA PCR array experimental work flow (left) and miRNA RT-PCR mechanism (right).

 

Performance data

 

Linear Range and Sensitivity (Total RNA)

   
 

Figure 2. Broad linear range and high sensitivity

     

Inter-Array Reproducibility

   
 

Figure 3. High inter-array reproducibility 

     

Single Nucleotide Mismatches

   
 

Figure 4. Specificity of miRNA detection

   

Catalog Arrays

miProfile™ Catalog miRNA qPCR Arrays

miProfile™ Catalog miRNA qPCR Arrays profile the expression of pre-selected miRNAs, such as the whole genome, cancer-related or disease-related miRNAs. The cancer-related or dissease-related miRNAs are carefully chosen for their close correlation with a specific cancer or disease based on a thorough literature search of peer-reviewed publications.

GeneCopoeia offer the following catalog miRNA qPCR arrays: 

miRNome qPCR arrays

Cancer miRNA qPCR arrays

Disease and focus-group miRNA qPCR arrays

 

 

Custom Arrays

miProfile™ Custom miRNA qPCR Arrays

For expression profiling of custom-specified miRNAs

miProfile™ Custom miRNA qPCR Arrays profile the expression of customer specified miRNAs. Each primer is designed using a proprietary algorithm and has been experimentally validated. Depending on the number of miRNAs and replicates to be analyzed, GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility:

  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.
  • Negative controls(NC): can be used as negative PCR control.

For the best performance, All-in-One™ miRNA First-Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

 

Key advantages

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Validated primers

  • Each primer is designed using a proprietary algorithm and has been experimentally validated

Flexible array design

  • GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate.

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer has been experimental validated for specific amplification
  • Broad linearity – Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicates

miRNA qPCR array custom services

Cat. No. Product name Species Number of miRNAs Number of plates Document
PAM-CS96 miProfile™ miRNA qPCR arrays custom services N/A Variable Variable Variable
 
 

 

Analysis Tool

GeneCopoeia offers convenient analysis tool for catalog miRNA qPCR arrays, as well as custom services. According to the product name and catalog number, download the matched analysis tools from following table.

Analysis Tool for Catalog Arrays

Please download an example for catalog array to see how to fill in the experimental data 

Cat. No. Product name Analysis Tool
QM001 miProfile™ human miRNome miRNA qPCR arrays
QM002 miProfile™ mouse miRNome miRNA qPCR arrays
QM003 miProfile™ human single-nucleotide mismatch miRNA qPCR arrays
QM004 miProfile™ human cancer miRNA qPCR arrays
QM005 miProfile™ human breast cancer miRNA qPCR arrays
QM006 miProfile™ human colorectal cancer miRNA qPCR arrays
QM007 miProfile™ human endometrial cancer miRNA qPCR arrays
QM008 miProfile™ human gastric cancer miRNA qPCR arrays
QM009 miProfile™ human hepatocellular carcinoma miRNA qPCR arrays
QM010 miProfile™ human lung cancer miRNA qPCR arrays
QM011 miProfile™ human melanoma miRNA qPCR arrays
QM012 miProfile™ human brain cancer miRNA qPCR arrays
QM013 miProfile™ human ovarian cancer miRNA qPCR arrays
QM014 miProfile™ human pancreatic cancer miRNA qPCR arrays
QM015 miProfile™ human prostate cancer miRNA qPCR arrays
QM016 miProfile™ human head and neck cancer miRNA qPCR arrays
QM017 miProfile™ human leukemia miRNA qPCR arrays
QM018 miProfile™ human bladder cancer miRNA qPCR arrays
QM019 miProfile™ human lymphoma miRNA qPCR arrays
QM020 miProfile™ mouse breast cancer miRNA qPCR arrays
QM021 miProfile™ mouse colorectal cancer miRNA qPCR arrays
QM022 miProfile™ mouse hepatocellular carcinoma miRNA qPCR arrays
QM023 miProfile™ mouse lung cancer miRNA qPCR arrays
QM024 miProfile™ mouse melanoma qPCR arrays
QM025 miProfile™ mouse brain cancer miRNA qPCR arrays
QM026 miProfile™ mouse ovarian cancer miRNA qPCR arrays
QM027 miProfile™ mouse pancreatic cancer miRNA qPCR arrays
QM028 miProfile™ mouse prostate cancer miRNA qPCR arrays
QM029 miProfile™ mouse head and neak cancer miRNA qPCR arrays
QM030 miProfile™ mouse leukemia miRNA qPCR arrays
QM031 miProfile™ human inflammatory miRNA qPCR array
QM032 miProfile™ human heart disease miRNA qPCR array
QM033 miProfile™ human immunopathology miRNA qPCR array
QM034 miProfile™ human IPS (Stem cell) miRNA qPCR arrays
QM035 miProfile™ human muscle disease miRNA qPCR array
QM036 miProfile™ human toxicology related miRNA qPCR array
QM037 miProfile™ human serum and plasma miRNA qPCR arrays
QM038 miProfile™ mouse inflammatory miRNA qPCR arrays
QM039 miProfile™ mouse heart disease miRNA qPCR arrays
QM040 miProfile™ mouse immunopathology miRNA qPCR arrays
QM041 miProfile™ mouse serum and plasma miRNA qPCR arrays
QM042 miProfile™ human miRNome miRNA qPCR arrays
QM043 miProfile™ mouse miRNome miRNA qPCR arrays

 

Analysis Tool for Custom Arrays

Operation guidance

 

Please upload your EXCEL file:







Download Datasheet template: Control Data.xls    Sample Data.xls

Publications

2016