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All-in-One™ miRNA qRT-PCR Reagent Kits and Validated Primers

Introduction

The All-in-One™ miRNA qRT-PCR reagent kits include the All-in-One miRNA qRT-PCR Detection Kits and the All-in-One miRNA First-Strand cDNA Synthesis Kits. When used with All-in-One Validated miRNA Primers or miProfile™ miRNA qPCR Arrays, the result is high-performance expression-profiling of all miRNAs.

A novel optimized blend of poly A polymerase and reverse transcriptase with an PAP/RT buffer that was specifically developed for the All-in-One Reverse Transcriptase Mix delivers maximum activity of both enzymes for robust first-strand cDNA synthesis from mature miRNAs. The All-in-One First-Strand cDNA Synthesis Kit is optimized for use with miProfile qPCR Arrays.

The All-in-One RT-PCR Detection Kit includes both RT and PCR reagents. It combines PCR technology and SYBR® Green to make fast and accurate quantification of mature miRNAs from as little as 10 pg of small RNA or 20 pg of total RNA samples. The kit is designed for use with All-in-One Validated miRNA Primers.

 

All-in-One miRNA assays provides sensitivity and specificity you can afford

  • Detect miRNAs from as little as 10 pg of small RNA or 20 pg of total RNA
  • Detect only mature miRNA
  • Compatible with GeneCopoeia miRNA qPCR arrays or validated miRNA-specific primers

 

All-in-One miRNA assays are fast and easy

 

Figure 1. Overview of steps involved in the All-in-One miRNA qRT-PCR Detection Kit. During 3' polyadenylation, M-MLV RTase and a unique Oligo dT Adaptor primer, reverse transcribes the poly A miRNAs. The kit qRT-PCR Mix, containing SYBR® Green specifically detects the reverse transcribed miRNAs.

 

Combine these tools with GeneCopoeia miRNA plasmid constructs and assays for comprehensive functional analysis studies.

 

*miRNA-specific primers sold separately.

Trademarks: SYBR® (Molecular Probes)

RT-PCR kit

Buy Catalog# Product Description Price
QP015 All-in-One™ miRNA qRT-PCR Detection Kit*
(20 RT and 200 qPCR reactions)
Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) $395
QP016 All-in-One™ miRNA qRT-PCR Detection Kit*
(60 RT and 600 qPCR reactions)
Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers (for use with miRNA qPCR primers) $985
QP017 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array
(20 RT reactions)
Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $225
QP018 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array
(60 RT reactions)
Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $515
  Variable All-in-One miRNA qPCR Primer
(200 reactions)
Validated miRNA human, mouse and rat primers

 

 

 + Download miRNA qPCR primer validation report sample (PDF)

 

Primer

All-in-One miRNA qPCR validated primers get the job done    

The All-in-One qPCR miRNA-specific primers are designed by a proprietary algorithm and validated for more than 700 human miRNAs.

When used in combination with the All-in-One SYBR® Green miRNA qRT-PCR kit, the All-in-One miRNA primers deliver reliable and reproducible high performance in quantitative PCR assays.

Primer validation

Figure 2A Figure 2B

Figure 2. All-in-One miRNA qPCR pimers are validated to generate a single amplification of the correct size for the targeted miRNA and to yield a single dissociation curve peak. A cDNA pool, containing reverse transcribed products from 10 human tissue total RNA samples was used as the validation template. qPCR was performed using 0.2 uM primer with 2X All-in-One qPCR Mix. (2A) validated result for amplification curve (2B) validated result for melting curve.

 + Download miRNA qPCR primer validation report sample (PDF)

qPCR array

miProfile™ miRNA qPCR arrays

For high-throughput profiling of miRNA expression

The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells.

Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer). The miRNA-specific primers have been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA.

Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.

 

            For profiling of miRNA whole genome expression

 

            For profiling of different types of cancer-related miRNA expression

 

            For profiling of disease or biological process-related miRNA expression

 

           For profiling of custom-selected miRNA expression

 

Publication

Publications

 

2016

 

2015

 

2014

 

2013

 

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