Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (for enhanced stability)

Firefly and Renilla Lucifease reporter vectors Only $99 When purchased with luciferase kits

- Advanced stability

 

Introduction

The Luc-Pair™ Duo-Luciferase Assay Kit 2.0 has been improved upon from the Luc-Pair™ Duo-Luciferase Assay Kit. With enhanced stability, it provides a more flexible system in the same convenient 96-well plate format as before for measuring firefly and Renilla luciferases sequentially. Due to its enhanced stability, the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 is the ideal solution for measuring the inhibitory effect of a miRNA on a target sequence. 

 

Key features

  • Enhanced stability
  • Greater flexibility
  • High sensitivity in a large dynamic range
  • Great for any mammalian cell line
  • Low background luminescence in the system
  • Better reproducibility due to a longer lasting signal
  • Simple to use and budget friendly
  • Optimized for high-throughput screening

 

GeneCopoeia also offers the Luc-Pair™ Duo-Luciferase HS Assay Kit for applications requiring high sensitvity luciferase readings.

 

Applications and advantages

  • High stability — Luciferase activity remains greater than 80% for 30 minutes after addition of the enzymatic substrates.
  • Ideal for studying the interaction of precursor miRNA clones with miRNA 3' UTR target sequence clones
  • Easy to use — less hands-on time and quick results
  • Suitable for micro-plate format — save time and money

 

Figure 1. Stability of firefly luciferase and Renilla luciferase signals using GeneCopoeia (GCI) Luc-Pair Duo-Luciferase Assay Kit 2.0. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. A.) The FLuc and RLuc activity was measured as described in the procedure. B.)The Competitor’s dual-luciferase assay kit was used in comparison.

 

Figure 2. Linear relationship between emitted light and amount of luciferase expression vector transfected in HEK293. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. The FLuc (A) and RLuc (B) activities were measured in lysates from different numbers of cells after incubation for 3 min as described in the procedure.

 

To Order

Buy Catalog# Product Description Price
LF003 Luc-Pair Duo-Luciferase Assay Kits 2.0 (1000rxns) Detects Firefly luciferase (FLuc) and Renilla luciferase (Rluc) $979
LF002 Luc-Pair Duo-Luciferase Assay Kits 2.0 (300rxns) Detects Firefly luciferase (FLuc) and Renilla luciferase (Rluc) $357
LF001 Luc-Pair Duo-Luciferase Assay Kits 2.0 (100rxns) Detects Firefly luciferase (FLuc) and Renilla luciferase (Rluc) $149
LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $40

 

  User manual MSDS
 

 

Empty Firefly luciferase cloning vectors (only $99 when purchased together with luciferase kits)

Buy Catalog# Product Description promoter Reporter gene Tracking gene Selection marker Size Price
ZX001 pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector N/A* Firefly luciferase (hLuc) Renilla luciferase (Rluc) Puromycin (10µg) $495
ZX002 pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector SV40 Firefly luciferase (hLuc) Renilla luciferase (Rluc) N/A* (10µg) $495
ZX003 pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector miniCMV Firefly luciferase (hLuc) Renilla luciferase (Rluc) Puromycin (10µg) $495

 

 

Publications

2016