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Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (for enhanced stability)

Advanced stability

 

Introduction

The Luc-Pair™ Duo-Luciferase Assay Kit 2.0 has been improved upon from the Luc-Pair™ Duo-Luciferase Assay Kit. With enhanced stability, it provides a more flexible system in the same convenient 96-well plate format as before for measuring firefly and Renilla luciferases sequentially. Due to its enhanced stability, the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 is the ideal solution for measuring the inhibitory effect of a miRNA on a target sequence. 

 

Key features

  • Enhanced stability
  • Greater flexibility
  • High sensitivity in a large dynamic range
  • Great for any mammalian cell line
  • Low background luminescence in the system
  • Better reproducibility due to a longer lasting signal
  • Simple to use and budget friendly
  • Optimized for high-throughput screening

 

GeneCopoeia also offers the Luc-Pair™ Duo-Luciferase HS Assay Kit for applications requiring high sensitvity luciferase readings.

 

Applications and advantages

  • High stability — Luciferase activity remains greater than 80% for 30 minutes after addition of the enzymatic substrates.
  • Ideal for studying the interaction of precursor miRNA clones with miRNA 3' UTR target sequence clones
  • Easy to use — less hands-on time and quick results
  • Suitable for micro-plate format — save time and money

 

Figure 1. Stability of firefly luciferase and Renilla luciferase signals using GeneCopoeia (GCI) Luc-Pair Duo-Luciferase Assay Kit 2.0. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. A.) The FLuc and RLuc activity was measured as described in the procedure. B.)The Competitor’s dual-luciferase assay kit was used in comparison.

 

Figure 2. Linear relationship between emitted light and amount of luciferase expression vector transfected in HEK293. HEK 293 cells were transfected with GeneCopoeia pEZX-MT06 miRNA reporter vector for 48 hours. The FLuc (A) and RLuc (B) activities were measured in lysates from different numbers of cells after incubation for 3 min as described in the procedure.

 

To Order

Buy Catalog# Product Description Price
LF003 Luc-Pair Duo-Luciferase Assay Kits 2.0 (1000rxns) Detects Firefly luciferase (FLuc) and Renilla luciferase (Rluc) $979
LF002 Luc-Pair Duo-Luciferase Assay Kits 2.0 (300rxns) Detects Firefly luciferase (FLuc) and Renilla luciferase (Rluc) $357
LF001 Luc-Pair Duo-Luciferase Assay Kits 2.0 (100rxns) Detects Firefly luciferase (FLuc) and Renilla luciferase (Rluc) $149
LF003-01 Luc-Lysis buffer(10×),10ml Compatible with Luc-Pair™ Duo-Luciferase Assay Kit 2.0 and Luc-Pair™ Firefly Luciferase HS Assay Kit $40

 

  User manual MSDS
 

 

Empty Firefly luciferase cloning vectors (Discounted price – $199)

Buy Catalog# Product Description promoter Reporter gene Tracking gene Selection marker Size Price
ZX001 pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector N/A* Firefly luciferase (hLuc) Renilla luciferase (Rluc) Puromycin (10µg) $495
ZX002 pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector SV40 Firefly luciferase (hLuc) Renilla luciferase (Rluc) N/A* (10µg) $495
ZX003 pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector miniCMV Firefly luciferase (hLuc) Renilla luciferase (Rluc) Puromycin (10µg) $495

 

 

FAQs

Frequently Asked Questions: Luciferase Assays

Answer: “RLU” stands for “Relative Light Unit”. Luminometers do not measure light output, in terms of numbers of photons, directly.
Answer: All GeneCopoeia luciferase kits are compatible with all known luminometers.
Answer: All GeneCopoeia luciferase reagents are stable for at least 6 months if stored at -20°C.
Answer: To prevent loss of activity, it is best to avoid repeated freezing and thawing. We recommend pipetting small aliquots into new, clean, sterile tubes and placing those aliquots at -20°C. Once you have thawed one of the aliquots, discard it instead of putting it back in the freezer.
Answer: The Gaussia luciferase substrate, coelenterazine, is also the substrate for Renilla luciferase, so it is compatible with Renilla. However, coelenterazine cannot be used with Firefly (Photinus pyralis) luciferase, which instead uses luciferin as its substrate.
Answer: Unfortunately, the individual components of GeneCopoeia's luciferase kits cannot be purchased separately at this time.
Answer: Typically, between 12 and 24 hours.
Answer: The Luc-Pair™ Duo-Luciferase Assay Kit 2.0 (For Enhanced Stability), Luc-Pair™ Duo-Luciferase Assay Kit HT (For High Throughput), Luc-Pair™ Firefly Luciferase HT Assay Kit, and Luc-Pair™ Renilla Luciferase HT Assay Kit (For High Throuhput), which provide greater signal stability, are most useful when assaying a relatively large number of samples. Their enhanced signal stability means that there will be little sample-to-sample variation due to signal decay. Conversely, the Luc-Pair™ Duo-Luciferase HS Assay Kit (For High Sensitivity), Luc-Pair™ Firefly Luciferase HS Assay Kit (For High Sensitivity), and Luc-Pair™ Renilla Luciferase HS Assay Kit (For High Sensitivity) are used when maximal signal intensity is required, but the trade-off is that the signal decays more rapidly under typical laboratory conditions. These kits are not appropriate for measuring the activities of large numbers of samples, unless sample injectors are used.

 

Publications

2017

2016