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miExpress™ Precursor miRNA Expression Clones

Introduction

MicroRNAs (miRNAs) are small, non-coding single stranded RNA molecules found in eukaryotic organisms. They are highly conserved and usually 21-23 nucleotides in length. miRNAs are involved in almost all cellular functions and regulate gene expression by binding to the 3' untranslated regions (3' UTRs) of targeted mRNAs. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

GeneCopoeia offers miExpress precursor miRNA expression clones in lentiviral and non-viral vectors with eGFP reporter gene. Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types.

 

Advantages         

 Full coverage

  • All known human, mouse and rat miRNAs in the current miRBase covered.

Flexible delivery systems

  • Lentiviral vectors allow efficient transduction and stable integration into the host genome of non-dividing and difficult to transfect cells target cells
  • Non-viral vectors allow either transient or stable transfection

Optimized expression cassettes

  • Expression cassettes have been fully sequenced and optimized for high miRNA expression of precursor miRNA and maturation of miRNA inside cells

Selection markers and reporters

  • An eGFP reporter is designed to monitor transfection or transduction efficiency
  • Neomycin or Puromycin selection marker enables stable cell line selection

Figure 2: Role of precursor miRNA and miRNA 3' UTR target clones in miRNA regulation studies

 

Vectors

Precursor miRNA vector features

 
 
Vector
Tracking gene
Selection marker
Promoter
Type
pEZX-MR01 eGFP Neomycin H1 FIV
pEZX-MR03 eGFP Puromycin CMV HIV
pEZX-MR04 eGFP Puromycin CMV Mammalian
 
 

 

Precursor miRNA lentivirus

GeneCopoeia offers precursor miRNA lentivirus as a custom service for only $995 and delivered within 2-5 weeks.

 

Not finding what you need?  Feel free to contact us.

 

Clone Sets

miRNA precursor clone sets

Besides individual clones, GeneCopoeia also offers pre-made or custom-made miRNA precursor clone sets. Uniformity and high-quality clone sets make them ideal for large-scale high content and high-throughput screening studies in functional genomics, proteomics and systems biology.

 

Vector choices

Vector Vector Type Promoter Selection Marker Tag
pEZX-MR03 Mammalian expression (lentiviral) CMV Puromycin eGFP
pEZX-MR04 Mammalian expression CMV Puromycin eGFP

 

Clone sets by gene family in the vector of your choice

Please contact us at 1-866-360-9531 or inquiry@genecopoeia.com if you have any questions.

Vector and species:

More vector types are available

 

Human whole genome miRNA precursor clone set (Lentiviral)

Cat. No. Species Product miRNA Price
MPC-HG-MR03 Human Human whole genome miRNA  precursor clone set (lentiviral) 1690 Inquire
Custom order Various Custom-selected Various Inquire

 

Human cancer miRNA precursor clone sets (Lentiviral)

Cat.No. Species Product Cancer miRNA Price
MPC-HCY-MR03 Human  Human Bladder Cancer miRNA Precursor Clone Set (lentiviral) Bladder Cancer 56 Inquire
MPC-HCN-MR03 Human  Human Brain Cancer miRNA Precursor Clone Set (lentiviral) Brain Cancer 174 Inquire
MPC-HCB-MR03 Human  Human Breast Cancer miRNA Precursor Clone Set (lentiviral) Breast Cancer 144 Inquire
MPC-HCV-MR03 Human  Human Cervical Cancer miRNA Precursor Clone Set (lentiviral) Cervical Cancer 21 Inquire
MPC-HCI-MR03 Human  Human Cholangiocarcinoma miRNA Precursor Clone Set (lentiviral) Cholangiocarcinoma 26 Inquire
MPC-HCC-MR03 Human  Human Colorectal Cancer miRNA Precursor Clone Set (lentiviral) Colorectal Cancer 141 Inquire
MPC-HCW-MR03 Human  Human Endometrial Cancer miRNA Precursor Clone Set (lentiviral) Endometrial Cancer 72 Inquire
MPC-HCG-MR03 Human  Human Gastric Cancer miRNA Precursor Clone Set (lentiviral) Gastric Cancer 60 Inquire
MPC-HCT-MR03 Human  Human Head and Neck Cancer miRNA Precursor Clone Set (lentiviral) Head and Neck Cancer 141 Inquire
MPC-HCH-MR03 Human  Human Hepatocellular Carcinoma miRNA Precursor Clone Set (lentiviral) Hepatocellular Carcinoma 130 Inquire
MPC-HCK-MR03 Human  Human Kidney Cancer miRNA Precursor Clone Set (lentiviral) Kidney Cancer 61 Inquire
MPC-HCX-MR03 Human  Human Leukemia miRNA Precursor Clone Set (lentiviral) Leukemia 126 Inquire
MPC-HCL-MR03 Human  Human Lung Cancer miRNA Precursor Clone Set (lentiviral) Lung Cancer 134 Inquire
MPC-HCZ-MR03 Human  Human Lymphoma miRNA Precursor Clone Set (lentiviral) Lymphoma 80 Inquire
MPC-HCM-MR03 Human  Human Melanoma miRNA Precursor Clone Set (lentiviral) Melanoma 103 Inquire
MPC-HCE-MR03 Human  Human Mesothelioma miRNA Precursor Clone Set (lentiviral) Mesothelioma 20 Inquire
MPC-HCR-MR03 Human  Human Myeloma miRNA Precursor Clone Set (lentiviral) Myeloma 38 Inquire
MPC-HCO-MR03 Human  Human Ovarian Cancer miRNA Precursor Clone Set (lentiviral) Ovarian Cancer 138 Inquire
MPC-HCP-MR03 Human  Human Pancreatic Cancer miRNA Precursor Clone Set (lentiviral) Pancreatic Cancer 154 Inquire
MPC-HCA-MR03 Human  Human Pituitary Adenoma miRNA Precursor Clone Set (lentiviral) Pituitary Adenoma 27 Inquire
MPC-HCQ-MR03 Human  Human Prostate Cancer miRNA Precursor Clone Set (lentiviral) Prostate Cancer 120 Inquire
MPC-HCS-MR03 Human  Human Sarcoma miRNA Precursor Clone Set (lentiviral) Sarcoma 22 Inquire
MPC-HCJ-MR03 Human  Human Thyroid Carcinoma miRNA Precursor Clone Set (lentiviral) Thyroid Carcinoma 40 Inquire
MPC-HCU-MR03 Human  Human Uterine Leiomyoma miRNA Precursor Clone Set (lentiviral) Uterine Leiomyoma 30 Inquire
Custom order Various Custom-selected Various Various Inquire
Cat. No. Species Product miRNA Price
MPC-MG-MR03 Mouse Mouse genome miRNA precursor clone set (lentiviral) 926 Inquire
Custom order Various Custom-selected Various Inquire
Cat. No. Species Product miRNA Price
MPC-RG-MR03 Rat Rat genome miRNA precursor clone set (lentiviral) 169 Inquire
Custom order Various Custom-selected Various Inquire

Human whole genome miRNA precursor clone set (Lentiviral)

Cat. No. Species Product miRNA Price
MPC-HG-MR04 Human Human whole genome miRNA  precursor clone set (non-viral) 702 Inquire
Custom order Various Custom-selected Various Inquire

 

Human cancer miRNA precursor clone sets (Non-viral)

Cat.No. Species Product Cancer miRNA Price
MPC-HCY-MR04 Human  Human Bladder Cancer Precursor miRNA Clone Set (non-viral) Bladder Cancer 56 Inquire
MPC-HCN-MR04 Human  Human Brain Cancer Precursor miRNA Clone Set (non-viral) Brain Cancer 173 Inquire
MPC-HCB-MR04 Human  Human Breast Cancer Precursor miRNA Clone Set (non-viral) Breast Cancer 143 Inquire
MPC-HCV-MR04 Human  Human Cervical Cancer Precursor miRNA Clone Set (non-viral) Cervical Cancer 20 Inquire
MPC-HCI-MR04 Human  Human Cholangiocarcinoma Precursor miRNA Clone Set (non-viral) Cholangiocarcinoma 26 Inquire
MPC-HCC-MR04 Human  Human Colorectal Cancer Precursor miRNA Clone Set (non-viral) Colorectal Cancer 140 Inquire
MPC-HCW-MR04 Human  Human Endometrial Cancer Precursor miRNA Clone Set (non-viral) Endometrial Cancer 72 Inquire
MPC-HCG-MR04 Human  Human Gastric Cancer Precursor miRNA Clone Set (non-viral) Gastric Cancer 59 Inquire
MPC-HCT-MR04 Human  Human Head and Neck Cancer Precursor miRNA Clone Set (non-viral) Head and Neck Cancer 140 Inquire
MPC-HCH-MR04 Human  Human Hepatocellular Carcinoma Precursor miRNA Clone Set (non-viral) Hepatocellular Carcinoma 129 Inquire
MPC-HCK-MR04 Human  Human Kidney Cancer Precursor miRNA Clone Set (non-viral) Kidney Cancer 61 Inquire
MPC-HCX-MR04 Human  Human Leukemia Precursor miRNA Clone Set (non-viral) Leukemia 125 Inquire
MPC-HCL-MR04 Human  Human Lung Cancer Precursor miRNA Clone Set (non-viral) Lung Cancer 133 Inquire
MPC-HCZ-MR04 Human  Human Lymphoma Precursor miRNA Clone Set (non-viral) Lymphoma 80 Inquire
MPC-HCM-MR04 Human  Human Melanoma Precursor miRNA Clone Set (non-viral) Melanoma 102 Inquire
MPC-HCE-MR04 Human  Human Mesothelioma Precursor miRNA Clone Set (non-viral) Mesothelioma 20 Inquire
MPC-HCR-MR04 Human  Human Myeloma Precursor miRNA Clone Set (non-viral) Myeloma 37 Inquire
MPC-HCO-MR04 Human  Human Ovarian Cancer Precursor miRNA Clone Set (non-viral) Ovarian Cancer 136 Inquire
MPC-HCP-MR04 Human  Human Pancreatic Cancer Precursor miRNA Clone Set (non-viral) Pancreatic Cancer 154 Inquire
MPC-HCA-MR04 Human  Human Pituitary Adenoma Precursor miRNA Clone Set (non-viral) Pituitary Adenoma 27 Inquire
MPC-HCQ-MR04 Human  Human Prostate Cancer Precursor miRNA Clone Set (non-viral) Prostate Cancer 120 Inquire
MPC-HCS-MR04 Human  Human Sarcoma Precursor miRNA Clone Set (non-viral) Sarcoma 22 Inquire
MPC-HCJ-MR04 Human  Human Thyroid Carcinoma Precursor miRNA Clone Set (non-viral) Thyroid Carcinoma 40 Inquire
MPC-HCU-MR04 Human  Human Uterine Leiomyoma Precursor miRNA Clone Set (non-viral) Uterine Leiomyoma 30 Inquire
Custom order Various Custom-selected Various Various Inquire
Cat. No. Species Product miRNA Price
MPC-MG-MR04 Mouse Mouse genome miRNA precursor clone set (non-viral) 562 Inquire
Custom order Various Custom-selected Various Inquire
Cat. No. Species Product miRNA Price
MPC-RG-MR04 Rat Rat genome miRNA precursor clone set (non-viral) 314 Inquire
Custom order Various Custom-selected Various Inquire

 

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Publications

 

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